Gold Nanoparticles with Asymmetric Polymerase Chain Reaction for Colorimetric Detection of DNA Sequence

2012 ◽  
Vol 84 (3) ◽  
pp. 1253-1258 ◽  
Author(s):  
Hua Deng ◽  
Yi Xu ◽  
Yanhua Liu ◽  
Zhijun Che ◽  
Huilin Guo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Dna Sequence ◽  
Colorimetric Detection ◽  
Chain Reaction ◽  
Asymmetric Polymerase Chain Reaction ◽  
Polymerase Chain

DNA sequence of the androgen receptor in prostatic tumor cell lines and tissue specimens assessed by means of the polymerase chain reaction

The Prostate ◽  
1993 ◽  
Vol 22 (1) ◽  
pp. 11-22 ◽  
Author(s):  
Zoran Culig ◽  
Helmut Klocker ◽  
Johannes Eberle ◽  
Felizia Kaspar ◽  
Alfred Hobisch ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Androgen Receptor ◽  
Tumor Cell ◽  
Dna Sequence ◽  
Cell Lines ◽  
Tumor Cell Lines ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Prostatic Tumor ◽  
Tissue Specimens

The effect of gold nanoparticles on the diagnostic polymerase chain reaction technique for equine herpes virus 1 (EHV-1)

RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54898-54903 ◽  
Author(s):  
Dalia M. El-Husseini ◽  
Nashwa M. Helmy ◽  
Reham H. Tammam
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Herpes Virus ◽  
Polymerase Chain Reaction Technique ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Herpès Virus

We experimented the effect of 15 nm unmodified citrate coated GNPs on the key PCR reactants to see if these would enhance the overall outcomes of the reaction. Thus, the optimized GNPs-assisted PCR could be used for more efficient diagnosis of EHV-1.

Gold nanoparticles alter Taq DNA polymerase activity during polymerase chain reaction

RSC Advances ◽  
2013 ◽  
Vol 3 (43) ◽  
pp. 20793 ◽  
Author(s):  
Samir Mandal ◽  
Maidul Hossain ◽  
T. Muruganandan ◽  
Gopinatha Suresh Kumar ◽  
Keya Chaudhuri
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Dna Polymerase ◽  
Polymerase Activity ◽  
Chain Reaction ◽  
Taq Dna Polymerase ◽  
Polymerase Chain

Sensitive DNA detection by polymerase chain reaction with gold nanoparticles

2018 ◽  
Vol 1038 ◽  
pp. 105-111 ◽  
Author(s):  
Li Zou ◽  
Ruidi Shen ◽  
Liansheng Ling ◽  
Gongke Li
Keyword(s):  
Polymerase Chain Reaction ◽  
Gold Nanoparticles ◽  
Dna Detection ◽  
Chain Reaction ◽  
Polymerase Chain

Polymerase Chain Reaction Assay for Detection of Escherichia coli O157:H7 and Escherichia coli O157:H−

2002 ◽  
Vol 65 (1) ◽  
pp. 5-11 ◽  
Author(s):  
TAKAHISA MIYAMOTO ◽  
NATSUKO ICHIOKA ◽  
CHIE SASAKI ◽  
HIROSHI KOBAYASHI ◽  
KEN-ICHI HONJOH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Polymerase Chain Reaction ◽  
Dna Sequence ◽  
Escherichia Coli O157 ◽  
Chain Reaction ◽  
Pcr Assay ◽  
E Coli ◽  
Pcr Product ◽  
Genomic Dnas ◽  
Polymerase Chain

The DNA band patterns generated by polymerase chain reaction (PCR) using the du2 primer and template DNAs from various strains of Escherichia coli and non–E. coli bacteria were compared. Among three to five prominent bands produced, the three bands at about 1.8, 2.7, and 5.0 kb were detected in all of the E. coli O157 strains tested. Some nonpathogenic E. coli and all pathogenic E. coli except E. coli O157 showed bands at 1.8 and 5.0 kb. It seems that the band at 2.7 kb is specific to E. coli O157. Sequence analysis of the 2.7-kb PCR product revealed the presence of a DNA sequence specific to E. coli O157:H− and E. coli O157:H7. Since the DNA sequence from base 15 to base 1008 of the PCR product seems to be specific to E. coli O157, a PCR assay was carried out with various bacterial genomic DNAs and O157-FHC1 and O157-FHC2 primers that amplified the region between base 23 and base 994 of the 2.7-kb PCR product. A single band at 970 bp was clearly detected in all of the strains of E. coli O157:H− and E. coli O157:H7 tested. However, no band was amplified from template DNAs from other bacteria, including both nonpathogenic and pathogenic E. coli except E. coli O157. All raw meats inoculated with E. coli O157:H7 at 3 × 100 to 3.5 × 102 CFU/25 g were positive both for our PCR assay after cultivation in mEC-N broth at 42°C for 18 h and for the conventional cultural method.

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