Designing Electrochemical Interfaces with Functionalized Magnetic Nanoparticles and Wrapped Carbon Nanotubes as Platforms for the Construction of High-Performance Bienzyme Biosensors

2011 ◽  
Vol 83 (20) ◽  
pp. 7807-7814 ◽  
Author(s):  
Marcos Eguílaz ◽  
Reynaldo Villalonga ◽  
Paloma Yáñez-Sedeño ◽  
José M. Pingarrón
Keyword(s):  
Carbon Nanotubes ◽  
Magnetic Nanoparticles ◽  
High Performance ◽  
Functionalized Magnetic Nanoparticles ◽  
Electrochemical Interfaces

High-Performance Functionalized Magnetic Nanoparticles with Tailored Sizes and Shapes for Localized Hyperthermia Applications

2021 ◽  
Author(s):  
Izabell Crăciunescu ◽  
Petru Palade ◽  
Nicuşor Iacob ◽  
George Marian Ispas ◽  
Anda Elena Stanciu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
High Performance ◽  
Functionalized Magnetic Nanoparticles

Rapid screening and analysis of alcohol dehydrogenase binders from Glycyrrhiza uralensis root extract using functionalized magnetic nanoparticles coupled with HPLC−MS/MS

2013 ◽  
Vol 91 (11) ◽  
pp. 1147-1154 ◽  
Author(s):  
Miao Chen ◽  
Liangliang Liu ◽  
Xiaoqing Chen
Keyword(s):  
Magnetic Nanoparticles ◽  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Relative Activity ◽  
Binding Activity ◽  
Glycyrrhiza Uralensis ◽  
Rapid Screening ◽  
Root Extract ◽  
Covalent Bonds ◽  
Functionalized Magnetic Nanoparticles

Alcohol dehydrogenase (ADH) is a key enzyme that converts ethanol into acetaldehyde. Its binders could be used to efficiently treat human toxic alcohol poisoning and suppress the acetaldehyde accumulation in alcohol hypersensitive alcoholics. In the present study, a new assay based on ADH-functionalized magnetic nanoparticles coupled with high-performance liquid chromatography − tandem mass spectrometry (HPLC−MS/MS) was developed for the rapid screening and identification of ADH binders. ADH was immobilized on silica-coated Fe3O4 magnetic nanoparticles via covalent bonds and the synthesized nanoparticles were characterized by TEM, XRD, FT-IR, and VSM. The amount of bound ADH onto magnetic nanoparticles was 80.6 μg/mg. The optimum temperature and pH for the enzymatic activity of immobilized ADH were 25 °C and 7.0, respectively. The relative activity of immobilized ADH remained 68.14% after 10 times of recycle, which exhibited good durability. Nine compounds with ADH-binding activity were screened and identified in Glycyrrhiza uralensis root extracts, among which seven compounds were first screened and identified. Experimental results proved that the proposed method could rapidly screen ADH binders from complex mixtures.

Functionalized magnetic nanoparticles for biomedical applications

2015 ◽  
Vol 21 (42) ◽  
pp. 6038-6054 ◽  
Author(s):  
Dragoș Gudovan ◽  
Paul Balaure ◽  
Dan Mihăiescu ◽  
Adrian Fudulu ◽  
Bogdan Purcăreanu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Biomedical Applications ◽  
Functionalized Magnetic Nanoparticles
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