Toward Label-Free Optical Fractionation of Blood—Optical Force Measurements of Blood Cells

2011 ◽  
Vol 83 (14) ◽  
pp. 5666-5672 ◽  
Author(s):  
Colin G. Hebert ◽  
Alex Terray ◽  
Sean J. Hart
Keyword(s):  
Blood Cells ◽  
Optical Force ◽  
Label Free ◽  
Force Measurements

Label-Free Detection of Bacillus anthracis Spore Uptake in Macrophage Cells Using Analytical Optical Force Measurements

2017 ◽  
Vol 89 (19) ◽  
pp. 10296-10302 ◽  
Author(s):  
Colin G. Hebert ◽  
Sean Hart ◽  
Tomasz A. Leski ◽  
Alex Terray ◽  
Qin Lu
Keyword(s):  
Bacillus Anthracis ◽  
Optical Force ◽  
Label Free ◽  
Force Measurements ◽  
Macrophage Cells ◽  
Label Free Detection

Label-Free Monitoring of Cell Signalling Processes Through AFM-Based Force Measurements

2011 ◽  
pp. 353-373
Author(s):  
Charles Cuerrier ◽  
Elie Simard ◽  
Charles-Antoine Lamontagne ◽  
Julie Boucher ◽  
Yannick Miron ◽  
...  
Keyword(s):  
Cell Signalling ◽  
Label Free ◽  
Force Measurements

Classification of blood cells and tumor cells using label-free ultrasound and photoacoustics

2015 ◽  
Vol 87 (8) ◽  
pp. 741-749 ◽  
Author(s):  
Eric M. Strohm ◽  
Michael C. Kolios
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
Label Free

scholarly journals Optical Force Measurements Illuminate Dynamics of Escherichia coli in Viscous Media

2020 ◽  
Vol 8 ◽  
Author(s):  
Declan J. Armstrong ◽  
Timo A. Nieminen ◽  
Itia Favre-Bulle ◽  
Alexander B. Stilgoe ◽  
Isaac C. D. Lenton ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Optical Force ◽  
Force Measurements ◽  
Viscous Media

scholarly journals Microfluidic Separation of Blood Cells Based on the Negative Dielectrophoresis Operated by Three Dimensional Microband Electrodes

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 833
Author(s):  
Tomoyuki Yasukawa ◽  
Junko Yamada ◽  
Hitoshi Shiku ◽  
Tomokazu Matsue ◽  
Masato Suzuki
Keyword(s):  
Electric Field ◽  
Blood Cells ◽  
White Blood Cells ◽  
Three Dimensional ◽  
Leukemia Cell ◽  
Leukemia Cell Line ◽  
Acute Monocytic Leukemia ◽  
Label Free ◽  
Monocytic Leukemia ◽  
Insulating Layer

A microfluidic device is presented for the continuous separation of red blood cells (RBCs) and white blood cells (WBCs) in a label-free manner based on negative dielectrophoresis (n-DEP). An alteration of the electric field, generated by pairs of slanted electrodes (separators) that is fabricated by covering parts of single slanted electrodes with an insulating layer is used to separate cells by their sizes. The repulsive force of n-DEP formed by slanted electrodes prepared on both the top and bottom substrates led to the deflection of the cell flow in lateral directions. The presence of gaps covered with an insulating layer for the electric field on the electrodes allows the passing of RBCs through gaps, while relatively large WBCs (cultured cultured human acute monocytic leukemia cell line (THP-1 cells)) flowed along the slanted separator without passing through the gaps and arrived at an edge in the channel. The passage efficiency for RBCs through the gaps and the arrival efficiency for THP-1 cells to the upper edge in the channel were estimated and found to be 91% and 93%, respectively.

scholarly journals Feeling the Force: Measurements of Platelet Contraction and Their Diagnostic Implications

2018 ◽  
Vol 45 (03) ◽  
pp. 285-296 ◽  
Author(s):  
Evelyn Williams ◽  
Oluwamayokun Oshinowo ◽  
Abhijit Ravindran ◽  
Wilbur Lam ◽  
David Myers
Keyword(s):  
Mechanical Properties ◽  
Heart Disease ◽  
Ischemic Heart Disease ◽  
Material Properties ◽  
Blood Cells ◽  
Ischemic Heart ◽  
Force Measurements ◽  
Blood Clots ◽  
Order Of Magnitude

AbstractIn addition to the classical biological and biochemical framework, blood clots can also be considered as active biomaterials composed of dynamically contracting platelets, nascent polymeric fibrin that functions as a matrix scaffold, and entrapped blood cells. As platelets sense, rearrange, and apply forces to the surrounding microenvironment, they dramatically change the material properties of the nascent clot, increasing its stiffness by an order of magnitude. Hence, the mechanical properties of blood clots are intricately tied to the forces applied by individual platelets. Research has also shown that the pathophysiological changes in clot mechanical properties are associated with bleeding and clotting disorders, cancer, stroke, ischemic heart disease, and more. By approaching the study of hemostasis and thrombosis from a biophysical and mechanical perspective, important insights have been made into how the mechanics of clotting and the forces applied by platelets are linked to various diseases. This review will familiarize the reader with a mechanics framework that is contextualized with relevant biology. The review also includes a discussion of relevant tools used to study platelet forces either directly or indirectly, and finally, concludes with a summary of potential links between clotting forces and disease.

scholarly journals Noninvasive imaging of flowing blood cells using label-free spectrally encoded flow cytometry

2012 ◽  
Vol 3 (6) ◽  
pp. 1455 ◽  
Author(s):  
Lior Golan ◽  
Daniella Yeheskely-Hayon ◽  
Limor Minai ◽  
Eldad J Dann ◽  
Dvir Yelin
Keyword(s):  
Flow Cytometry ◽  
Blood Cells ◽  
Noninvasive Imaging ◽  
Label Free ◽  
Flowing Blood ◽  
Spectrally Encoded

scholarly journals Optical force measurements utilizing Lanthanide Binding Tags

2009 ◽  
Vol 96 (3) ◽  
pp. 402a-403a
Author(s):  
Walter Sandtner ◽  
Bernhard Egwolf ◽  
Benoit Roux ◽  
Ana M. Correa ◽  
Francisco Bezanilla
Keyword(s):  
Optical Force ◽  
Force Measurements ◽  
Lanthanide Binding Tags
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