scholarly journals Push–Pull Perfusion Sampling with Segmented Flow for High Temporal and Spatial Resolution in Vivo Chemical Monitoring

2011 ◽  
Vol 83 (13) ◽  
pp. 5207-5213 ◽  
Author(s):  
Thomas R. Slaney ◽  
Jing Nie ◽  
Neil D. Hershey ◽  
Prasanna K. Thwar ◽  
Jennifer Linderman ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Segmented Flow ◽  
Chemical Monitoring ◽  
Temporal And Spatial

In vivo biochemistry: quantifying ion and metabolite levels in individual cells or cultures of yeast

2011 ◽  
Vol 438 (1) ◽  
pp. 1-10 ◽  
Author(s):  
Clara Bermejo ◽  
Jennifer C. Ewald ◽  
Viviane Lanquar ◽  
Alexander M. Jones ◽  
Wolf B. Frommer
Keyword(s):  
Spatial Resolution ◽  
Time Resolution ◽  
Subcellular Distribution ◽  
Recent Progress ◽  
Yeast Cells ◽  
Fluorescent Indicators ◽  
Cellular Processes ◽  
The Past ◽  
Temporal And Spatial

Over the past decade, we have learned that cellular processes, including signalling and metabolism, are highly compartmentalized, and that relevant changes in metabolic state can occur at sub-second timescales. Moreover, we have learned that individual cells in populations, or as part of a tissue, exist in different states. If we want to understand metabolic processes and signalling better, it will be necessary to measure biochemical and biophysical responses of individual cells with high temporal and spatial resolution. Fluorescence imaging has revolutionized all aspects of biology since it has the potential to provide information on the cellular and subcellular distribution of ions and metabolites with sub-second time resolution. In the present review we summarize recent progress in quantifying ions and metabolites in populations of yeast cells as well as in individual yeast cells with the help of quantitative fluorescent indicators, namely FRET metabolite sensors. We discuss the opportunities and potential pitfalls and the controls that help preclude misinterpretation.

scholarly journals Toward Quantitative “In Vivo Biochemistry” with Fluorescence Fluctuation Spectroscopy

2010 ◽  
Vol 21 (24) ◽  
pp. 4306-4311 ◽  
Author(s):  
Brian D. Slaughter ◽  
Rong Li
Keyword(s):  
Protein Dynamics ◽  
Spatial Resolution ◽  
Cell Biology ◽  
Molecular Mechanisms ◽  
Quantitative Description ◽  
Fluorescence Fluctuation Spectroscopy ◽  
Molecular Concentration ◽  
Pros And Cons ◽  
Temporal And Spatial

Quantitative description of protein dynamics and interactions in vivo with temporal and spatial resolution is a key step in dissecting molecular mechanisms in cell biology. Fluorescence fluctuation spectroscopy (FFS) has recently emerged as a powerful in vivo tool for assessing molecular concentration and movement and formation of hetero- and homo-oligomeric complexes. This article discusses point FFS-based analysis methods that have proven useful to cell biologists, focusing on the kinds of information they provide, their pros and cons, and the basic instrumentation required. Along the way, we describe briefly a few recent examples where these analyses have helped address important biological questions.

scholarly journals In vivo interactome profiling by enzyme‐catalyzed proximity labeling

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yangfan Xu ◽  
Xianqun Fan ◽  
Yang Hu
Keyword(s):  
State Of The Art ◽  
Catalytic Efficiency ◽  
Biological Processes ◽  
Protein Protein Interaction ◽  
Current State ◽  
Protein Interactome ◽  
Potential Applications ◽  
Protein Protein Interaction Networks ◽  
Temporal And Spatial

AbstractEnzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases.

scholarly journals Semi-automated classification of colonial Microcystis by FlowCAM imaging flow cytometry in mesocosm experiment reveals high heterogeneity during seasonal bloom

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yersultan Mirasbekov ◽  
Adina Zhumakhanova ◽  
Almira Zhantuyakova ◽  
Kuanysh Sarkytbayev ◽  
Dmitry V. Malashenkov ◽  
...  
Keyword(s):  
Machine Learning ◽  
Flow Cytometry ◽  
Spatial Resolution ◽  
Mesocosm Experiment ◽  
Imaging Flow Cytometry ◽  
Leibler Divergence ◽  
Temporal And Spatial ◽  
High Level ◽  
Training Sets

AbstractA machine learning approach was employed to detect and quantify Microcystis colonial morphospecies using FlowCAM-based imaging flow cytometry. The system was trained and tested using samples from a long-term mesocosm experiment (LMWE, Central Jutland, Denmark). The statistical validation of the classification approaches was performed using Hellinger distances, Bray–Curtis dissimilarity, and Kullback–Leibler divergence. The semi-automatic classification based on well-balanced training sets from Microcystis seasonal bloom provided a high level of intergeneric accuracy (96–100%) but relatively low intrageneric accuracy (67–78%). Our results provide a proof-of-concept of how machine learning approaches can be applied to analyze the colonial microalgae. This approach allowed to evaluate Microcystis seasonal bloom in individual mesocosms with high level of temporal and spatial resolution. The observation that some Microcystis morphotypes completely disappeared and re-appeared along the mesocosm experiment timeline supports the hypothesis of the main transition pathways of colonial Microcystis morphoforms. We demonstrated that significant changes in the training sets with colonial images required for accurate classification of Microcystis spp. from time points differed by only two weeks due to Microcystis high phenotypic heterogeneity during the bloom. We conclude that automatic methods not only allow a performance level of human taxonomist, and thus be a valuable time-saving tool in the routine-like identification of colonial phytoplankton taxa, but also can be applied to increase temporal and spatial resolution of the study.

Potential of Arabic documentary sources for reconstructing past climate in the western Mediterranean region from AD 680 to 1815

The Holocene ◽  
2021 ◽  
pp. 095968362110332
Author(s):  
Yassin Meklach ◽  
Chantal Camenisch ◽  
Abderrahmane Merzouki ◽  
Ricardo Garcia Herrera
Keyword(s):  
Spatial Resolution ◽  
Mediterranean Region ◽  
Climate Extremes ◽  
Western Mediterranean ◽  
Historical Documents ◽  
Atmospheric Conditions ◽  
Documentary Sources ◽  
Drought Conditions ◽  
Temporal And Spatial ◽  
Climate Proxies

Archival records and historical documents offer direct observation of weather and atmospheric conditions and have the highest temporal and spatial resolution, and precise dating, of the available climate proxies. They also provide information about variables such as temperature, precipitation and climate extremes, as well as floods, droughts and storms. The present work studied Arab-Islamic documentary sources covering the western Mediterranean region (documents written by Arab-Islamic historians that narrate social, political and religious history) available for the period AD 680–1815. They mostly provide information on hydrometeorological events. In Iberia the most intense droughts were reported during AD 747–753, AD 814–822, AD 846–847, AD 867–874 and AD 914–915 and in the Maghreb AD 867–873, AD 898–915, AD 1104–1147, AD 1280–1340 and AD 1720–1815 had prevalent drought conditions. Intense rain episodes are also reported.

Interphalangeal Joint Cartilage: High-Spatial-Resolution in Vivo MR T2 Mapping—A Feasibility Study

Radiology ◽  
2004 ◽  
Vol 233 (1) ◽  
pp. 292-296 ◽  
Author(s):  
Jelena Lazovic-Stojkovic ◽  
Timothy J. Mosher ◽  
Harvey E. Smith ◽  
Qing X. Yang ◽  
Bernard J. Dardzinski ◽  
...  
Keyword(s):  
Feasibility Study ◽  
Spatial Resolution ◽  
High Spatial Resolution ◽  
T2 Mapping ◽  
Interphalangeal Joint ◽  
Joint Cartilage
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