Chemical, Thermal, and Electric Field Induced Unfolding of Single Protein Molecules Studied Using Nanopores

2011 ◽  
Vol 83 (13) ◽  
pp. 5137-5144 ◽  
Author(s):  
Kevin J. Freedman ◽  
Maike Jürgens ◽  
Anmiv Prabhu ◽  
Chi Won Ahn ◽  
Per Jemth ◽  
...  
Keyword(s):  
Electric Field ◽  
Protein Molecules ◽  
Single Protein

scholarly journals Highly Sensitive Protein Quantification by Direct Kinetic Fingerprinting of Single Protein Molecules

2021 ◽  
Vol 120 (3) ◽  
pp. 185a
Author(s):  
Tanmay Chatterjee ◽  
Achim Knappik ◽  
Erin Sandford ◽  
Muneesh Tewari ◽  
Sung Won Choi ◽  
...  
Keyword(s):  
Protein Quantification ◽  
Highly Sensitive ◽  
Protein Molecules ◽  
Single Protein

Measurement of Single Protein Molecules Using Digital ELISA

2013 ◽  
pp. 223-242 ◽  
Author(s):  
David M. Rissin ◽  
David H. Wilson ◽  
David C. Duffy
Keyword(s):  
Protein Molecules ◽  
Single Protein ◽  
Digital Elisa

scholarly journals Water-mediated influence of a crowded environment on internal vibrations of a protein molecule

2016 ◽  
Vol 18 (6) ◽  
pp. 4881-4890 ◽  
Author(s):  
Anna Kuffel ◽  
Jan Zielkiewicz
Keyword(s):  
Protein Molecule ◽  
Protein Molecules ◽  
Single Protein ◽  
Crowded Environment ◽  
Internal Vibrations

The influence of crowding on the protein inner dynamics is examined by putting a single protein molecule close to one or two neighboring protein molecules.

Selective transport of single protein molecules inside gold nanotubes

2012 ◽  
Vol 1238 ◽  
pp. 11-14 ◽  
Author(s):  
Changbei Ma ◽  
Rui Han ◽  
Shengda Qi ◽  
Edward S. Yeung
Keyword(s):  
Selective Transport ◽  
Protein Molecules ◽  
Single Protein

scholarly journals Dynamical Observations of Brownian Motions on Single Protein Molecules Using Diffracted Electron Tracking (DET)

2010 ◽  
Vol 98 (3) ◽  
pp. 589a
Author(s):  
Yuji C. Sasaki ◽  
Hiroshi Sekiguchi ◽  
Akihiro Ohsaki ◽  
Yasuhisa Hirohata ◽  
Akira Ishikawa
Keyword(s):  
Brownian Motions ◽  
Protein Molecules ◽  
Single Protein

scholarly journals Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffolding framework ordered under the influence of an electric field

2010 ◽  
Vol 8 (1) ◽  
pp. 41-50 ◽  
Author(s):  
Barbara Stopa ◽  
Barbara Piekarska ◽  
Leszek Konieczny ◽  
Marcin Król ◽  
Janina Rybarska ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Electric Field ◽  
Congo Red ◽  
Polarized Light ◽  
Sodium Dithionite ◽  
Ordered Structure ◽  
X Ray Diffraction ◽  
X Ray ◽  
Protein Molecules ◽  
Ordered State

AbstractThis study describes a technique which makes it possible to introduce the amyloid-like order to protein aggregates by using the scaffolding framework built from supramolecular, fibrillar Congo red structures arranged in an electric field. The electric field was used not only to obtain a uniform orientation of the charged dye fibrils, but also to make the fibrils long, compact and rigid due to the delocalization of pi electrons, which favors ring stacking and, as a consequence, results in an increased tendency to self-assemble. The protein molecules (immunoglobulin L chain lambda, ferritin) attached to this easily adsorbing dye framework assume its ordered structure. The complex precipitating as plate-like fragments shows birefringence in polarized light. The parallel organization of fibrils can be observed with an electron microscope. The dye framework may be removed via reduction with sodium dithionite, leaving the aggregated protein molecules in the ordered state, as confirmed by X-ray diffraction studies.

Fe3O4-in-silica super crystal of defined interstices for single protein molecules entrapment under magnetic flux

2010 ◽  
Vol 46 (36) ◽  
pp. 6699 ◽  
Author(s):  
Lin Ye ◽  
Chih Hao Yu ◽  
PengJu Jiang ◽  
Lin Qiu ◽  
Olivia T.W. Ng ◽  
...  
Keyword(s):  
Magnetic Flux ◽  
Protein Molecules ◽  
Single Protein
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