Bioimaging of Metals and Biomolecules in Mouse Heart by Laser Ablation Inductively Coupled Plasma Mass Spectrometry and Secondary Ion Mass Spectrometry

2010 ◽  
Vol 82 (22) ◽  
pp. 9528-9533 ◽  
Author(s):  
J. Sabine Becker ◽  
Uwe Breuer ◽  
Hui-Fang Hsieh ◽  
Tobias Osterholt ◽  
Usarat Kumtabtim ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Secondary Ion Mass Spectrometry ◽  
Mouse Heart ◽  
Inductively Coupled ◽  
Ion Mass Spectrometry ◽  
Plasma Mass Spectrometry ◽  
Secondary Ion

scholarly journals Technical note: Single-shell δ<sup>11</sup>B analysis of <i>Cibicidoides wuellerstorfi</i> using femtosecond laser ablation MC-ICPMS and secondary ion mass spectrometry

2020 ◽  
Author(s):  
Markus Raitzsch ◽  
Claire Rollion-Bard ◽  
Ingo Horn ◽  
Grit Steinhoefel ◽  
Albert Benthien ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Femtosecond Laser ◽  
Inductively Coupled Plasma ◽  
Secondary Ion Mass Spectrometry ◽  
Femtosecond Laser Ablation ◽  
Technical Note ◽  
Bulk Solution ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Abstract. The boron isotopic composition (δ11B) of benthic foraminifera provides a valuable tool to reconstruct past deep-water pH. As the abundance of monospecific species might be limited in sediments, microanalytical techniques can help to overcome this problem, but such studies on benthic foraminiferal δ11B are sparse. In addition, microanalytics provide information on the distribution of δ11B at high spatial resolution to increase the knowledge of e.g. biomineralization processes. For this study, we investigated the intra- and inter-shell δ11B variability of the epibenthic species Cibicidoides wuellerstorfi, which is widely used in paleoceanography, by secondary ion mass spectrometry (SIMS) and femtosecond laser ablation multicollector inductively coupled plasma mass spectrometry (LA-MC-ICPMS). While the average δ11B values obtained from these different techniques agree remarkably well with bulk solution values to within ± 0.1 ‰, a relatively large intra-shell variability was observed. Based on multiple measurements within single shells, the SIMS and LA data suggest median variations of 4.8 ‰ and 1.3 ‰ (2σ), respectively, where the larger spread for SIMS is attributed to the smaller volume of calcite being analyzed in each run. When analytical uncertainties and volume-dependent differences in δ11B variations are taken into account for these methods, the intra-shell variability is presumably in the order of ~ 3 ‰ and ~ 0.4 ‰ (2σ) on a ~ 20 µm and 100 µm scale, respectively. In comparison, the δ11B variability between shells exhibits a total range of ~ 3 ‰ for both techniques, suggesting that several shells need to be analyzed for accurate mean δ11B values. Based on a simple resampling method, we conclude that ~ 7 shells of C. wuellerstorfi must be analyzed using LA-MC-ICPMS to obtain an accurate average value within ± 0.5 ‰ (2σ) to resolve pH variations of ~ 0.1. Based on our findings, we suggest to prefer the conventional bulk solution MC-ICPMS over the in-situ methods for e.g. paleo-pH studies. However, SIMS and LA provide powerful tools for high-resolution paleoreconstructions, or for investigating ontogenetic trends in δ11B, possibly due to vital effects during chamber formation.

scholarly journals Technical note: Single-shell <i>δ</i><sup>11</sup>B analysis of <i>Cibicidoides wuellerstorfi</i> using femtosecond laser ablation MC-ICPMS and secondary ion mass spectrometry

2020 ◽  
Vol 17 (21) ◽  
pp. 5365-5375
Author(s):  
Markus Raitzsch ◽  
Claire Rollion-Bard ◽  
Ingo Horn ◽  
Grit Steinhoefel ◽  
Albert Benthien ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Femtosecond Laser ◽  
Inductively Coupled Plasma ◽  
Secondary Ion Mass Spectrometry ◽  
Femtosecond Laser Ablation ◽  
Technical Note ◽  
Bulk Solution ◽  
Ion Mass Spectrometry ◽  
Secondary Ion

Abstract. The boron isotopic composition (δ11B) of benthic foraminifera provides a valuable tool to reconstruct past deep-water pH. As the abundance of monospecific species might be limited in sediments, microanalytical techniques can help to overcome this problem, but such studies on benthic foraminiferal δ11B are sparse. In addition, microanalytics provide information on the distribution of δ11B at high spatial resolution to increase the knowledge of biomineralization processes, for example. For this study, we investigated the intra- and inter-shell δ11B variability of the epibenthic species Cibicidoides wuellerstorfi, which is widely used in paleoceanography, by secondary ion mass spectrometry (SIMS) and femtosecond laser ablation multicollector inductively coupled plasma mass spectrometry (LA-MC-ICPMS). While the average δ11B values obtained from these different techniques agree remarkably well with bulk solution values to within ±0.1 ‰, a relatively large intra-shell variability was observed. Based on multiple measurements within single shells, the SIMS and LA data suggest median variations of 4.8 ‰ and 1.3 ‰ (2σ), respectively, while the larger spread for SIMS is attributed to the smaller volume of calcite being analyzed in each run. When analytical uncertainties and volume-dependent differences in δ11B variations are taken into account for these methods, the intra-shell variability is estimated to be on the order of ∼3 ‰ and ∼0.4 ‰ (2σ) on a ∼20 and 100 µm scale, respectively. In comparison, the δ11B variability between shells exhibits a total range of ∼3 ‰ for both techniques, suggesting that several shells need to be analyzed for accurate mean δ11B values. Based on a simple resampling method, we conclude that ∼12 shells of C. wuellerstorfi must be analyzed using LA-MC-ICPMS to obtain an accurate average value within ±0.5 ‰ (2σ) to resolve pH variations of ∼0.1. Based on our findings, we suggest preferring the conventional bulk solution MC-ICPMS over the in situ methods for paleo-pH studies, for example. However, SIMS and LA provide powerful tools for high-resolution paleoreconstructions, or for investigating ontogenetic trends in δ11B.

Sign in / Sign up

Export Citation Format

Share Document