Real Time Observation of Chemical Reactions of Individual Metal Nanoparticles with High-Throughput Single Molecule Spectral Microscopy

2010 ◽  
Vol 82 (20) ◽  
pp. 8744-8749 ◽  
Author(s):  
Jing Cheng ◽  
Yang Liu ◽  
Xiaodong Cheng ◽  
Yan He ◽  
Edward S. Yeung
Keyword(s):  
Metal Nanoparticles ◽  
Real Time ◽  
Chemical Reactions ◽  
High Throughput ◽  
Single Molecule ◽  
Individual Metal ◽  
Time Observation ◽  
Real Time Observation

Real-time, single-molecule observation of biomolecular interactions inside nanophotonic zero mode waveguides

2021 ◽  
Author(s):  
Anastasiia Nemashkalo ◽  
Mary Elizabeth Phipps ◽  
Scott Patrick Hennelly ◽  
Peter Marvin Goodwin
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Ribosomal Rna ◽  
Protein Interactions ◽  
Zero Mode ◽  
Single Molecule Level ◽  
Sequencing By Synthesis ◽  
Time Observation ◽  
Real Time Observation

Abstract Living cells rely on numerous protein-protein, RNA-protein and DNA-protein interactions for processes such as gene expression, biomolecular assembly, protein and RNA degradation. Single-molecule microscopy and spectroscopy are ideal tools for real-time observation and quantification of nucleic acids-protein and protein-protein interactions. One of the major drawbacks of conventional single-molecule imaging methods is low throughput. Methods such as sequencing by synthesis utilizing nanofabrication and single-molecule spectroscopy have brought high throughput into the realm of single-molecule biology. The Pacific Biosceinces RS2 sequencer utilizes sequencing by synthesis within nanophotonic zero mode waveguides. A number of years ago this instrument was unlocked by Pacific Biosciences for custom use by researchers allowing them to monitor biological interactions at the single-molecule level with high throughput. In this capability letter we demonstrate the use of the RS2 sequencer for real time observation of DNA-to-RNA transcription and RNA-protein interactions. We use a relatively complex model – transcription of structured ribosomal RNA from E. coli and interactions of ribosomal RNA with ribosomal proteins. We also show evidence of observation of transcriptional pausing without the application of an external force (as is required for single-molecule pausing studies using optical traps). Overall, in the unlocked, custom mode, the RS2 sequencer can be used to address a wide variety of biological assembly and interaction questions at the single-molecule level with high throughput. This instrument is available for use at the Center for Integrated Nanotechnologies Gateway located at Los Alamos National Laboratory.

Real-space and real-time observation of a plasmon-induced chemical reaction of a single molecule

Science ◽  
2018 ◽  
Vol 360 (6388) ◽  
pp. 521-526 ◽  
Author(s):  
Emiko Kazuma ◽  
Jaehoon Jung ◽  
Hiromu Ueba ◽  
Michael Trenary ◽  
Yousoo Kim
Keyword(s):  
Real Time ◽  
Chemical Reaction ◽  
Single Molecule ◽  
Real Space ◽  
Time Observation ◽  
Real Time Observation

scholarly journals Real-Time Observation of DNA Recognition by the RNA-Guided Endonuclease Cas9 using Single-Molecule FRET

2015 ◽  
Vol 108 (2) ◽  
pp. 26a
Author(s):  
Digvijay Singh ◽  
Samuel H. Sternberg ◽  
Jingyi Fei ◽  
Jennifer A. Doudna ◽  
Taekjip Ha
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Dna Recognition ◽  
Single Molecule Fret ◽  
Time Observation ◽  
Real Time Observation

Real-time observation of single-molecule fluorescence in microdroplet streams

1997 ◽  
Vol 22 (16) ◽  
pp. 1265 ◽  
Author(s):  
M. D. Barnes ◽  
N. Lermer ◽  
C.-Y. Kung ◽  
W. B. Whitten ◽  
J. M. Ramsey ◽  
...  
Keyword(s):  
Real Time ◽  
Single Molecule ◽  
Single Molecule Fluorescence ◽  
Time Observation ◽  
Real Time Observation

scholarly journals Real-Time Observation of Multiple-Protein Complex Formation with Single-Molecule FRET

2013 ◽  
Vol 135 (28) ◽  
pp. 10254-10257 ◽  
Author(s):  
Woori Bae ◽  
Mal-Gi Choi ◽  
Changbong Hyeon ◽  
Yeon-Kyun Shin ◽  
Tae-Young Yoon
Keyword(s):  
Complex Formation ◽  
Real Time ◽  
Single Molecule ◽  
Protein Complex ◽  
Single Molecule Fret ◽  
Protein Complex Formation ◽  
Multiple Protein ◽  
Time Observation ◽  
Real Time Observation
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