Selective Enrichment and Analysis of Acidic Peptides and Proteins Using Polymeric Reverse Micelles and MALDI-MS

2010 ◽  
Vol 82 (20) ◽  
pp. 8686-8691 ◽  
Author(s):  
Nadnudda Rodthongkum ◽  
Yangbin Chen ◽  
S. Thayumanavan ◽  
Richard W. Vachet
Keyword(s):  
Reverse Micelles ◽  
Maldi Ms ◽  
Selective Enrichment ◽  
Acidic Peptides

scholarly journals Selective enrichment and sensitive detection of peptide and proteinbiomarkers in human serum using polymeric reverse micelles and MALDI-MS

The Analyst ◽  
2012 ◽  
Vol 137 (4) ◽  
pp. 1024-1030 ◽  
Author(s):  
Nadnudda Rodthongkum ◽  
Rajasekhar Ramireddy ◽  
S. Thayumanavan ◽  
W. Vachet Richard
Keyword(s):  
Human Serum ◽  
Reverse Micelles ◽  
Maldi Ms ◽  
Sensitive Detection ◽  
Selective Enrichment

On-Plate Selective Enrichment and Self-Desalting of Peptides/Proteins for Direct MALDI MS Analysis

2012 ◽  
Vol 84 (5) ◽  
pp. 2118-2123 ◽  
Author(s):  
Zhoufang Zeng ◽  
Yandong Wang ◽  
Shoulei Shi ◽  
Lifeng Wang ◽  
Xinhua Guo ◽  
...  
Keyword(s):  
Maldi Ms ◽  
Selective Enrichment ◽  
Ms Analysis

On-plate glycoproteins/glycopeptides selective enrichment and purification based on surface pattern for direct MALDI MS analysis

The Analyst ◽  
2013 ◽  
Vol 138 (10) ◽  
pp. 3032 ◽  
Author(s):  
Zhoufang Zeng ◽  
Yandong Wang ◽  
Xinhua Guo ◽  
Ling Wang ◽  
Nan Lu
Keyword(s):  
Maldi Ms ◽  
Surface Pattern ◽  
Selective Enrichment ◽  
Ms Analysis

scholarly journals Improved mass spectrometric detection of acidic peptides by variations in the functional group pKa values of reverse micelle extraction agents

The Analyst ◽  
2018 ◽  
Vol 143 (6) ◽  
pp. 1434-1443 ◽  
Author(s):  
Bo Zhao ◽  
Mahalia A. C. Serrano ◽  
Meizhe Wang ◽  
Tianying Liu ◽  
Mallory R. Gordon ◽  
...  
Keyword(s):  
Functional Groups ◽  
Aqueous Phase ◽  
Isoelectric Point ◽  
Reverse Micelle ◽  
Reverse Micelles ◽  
Functional Group ◽  
Selective Extraction ◽  
Mass Spectrometric ◽  
Acidic Peptides ◽  
Reverse Micelle Extraction

Functional groups in reverse micelles maintain their aqueous phase pKa and allow selective extraction of peptides according to isoelectric point.

Miniaturized Solid-Phase Extraction and Sample Preparation for MALDI MS Using a Microfabricated Integrated Selective Enrichment Target

2006 ◽  
Vol 5 (5) ◽  
pp. 1071-1081 ◽  
Author(s):  
Simon Ekström ◽  
Lars Wallman ◽  
Daniel Hök ◽  
György Marko-Varga ◽  
Thomas Laurell
Keyword(s):  
Sample Preparation ◽  
Solid Phase Extraction ◽  
Solid Phase ◽  
Maldi Ms ◽  
Phase Extraction ◽  
Selective Enrichment

Infrared investigation of water encapsulated in non ionic reverse micelles

2000 ◽  
Vol 10 (PR7) ◽  
pp. Pr7-215-Pr7-218 ◽  
Author(s):  
J.-B. Brubach ◽  
A. Mermet ◽  
A. Filabozzi ◽  
P. Colavita ◽  
A. Gerschel ◽  
...  
Keyword(s):  
Reverse Micelles

Unravelling a microbial synergy to boost caproate production via carboxylates chain elongation with ethanol

2019 ◽  
Vol 26 (2) ◽  
pp. 63-71
Author(s):  
Ling Leng ◽  
Ying Wang ◽  
Peixian Yang ◽  
Takashi Narihiro ◽  
Masaru Konishi Nobu ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Microbial Consortia ◽  
Chain Elongation ◽  
Desulfovibrio Vulgaris ◽  
Rrna Gene ◽  
Selective Enrichment ◽  
Microbial Network ◽  
Cost Efficient ◽  
Rrna Gene Sequencing

Chain elongation of volatile fatty acids for medium chain fatty acids production (e.g. caproate) is an attractive approach to treat wastewater anaerobically and recover resource simultaneously. Undefined microbial consortia can be tailored to achieve chain elongation process with selective enrichment from anaerobic digestion sludge, which has advantages over pure culture approach for cost-efficient application. Whilst the metabolic pathway of the dominant caproate producer, Clostridium kluyveri, has been annotated, the role of other coexisting abundant microbiomes remained unclear. To this end, an ethanol-acetate fermentation inoculated with fresh digestion sludge at optimal conditions was conducted. Also, physiological study, thermodynamics and 16 S rRNA gene sequencing to elucidate the biological process by linking the system performance and dominant microbiomes were integrated. Results revealed a possible synergistic network in which C. kluyveri and three co-dominant species, Desulfovibrio vulgaris, Fusobacterium varium and Acetoanaerobium sticklandii coexisted. D. vulgaris and A. sticklandii (F. varium) were likely to boost the carboxylates chain elongation by stimulating ethanol oxidation and butyrate production through a syntrophic partnership with hydrogen (H2) serving as an electron messenger. This study unveils a synergistic microbial network to boost caproate production in mixed culture carboxylates chain elongation.

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