Ion Mobility-Mass Spectrometry Reveals the Influence of Subunit Packing and Charge on the Dissociation of Multiprotein Complexes

2010 ◽  
Vol 82 (23) ◽  
pp. 9702-9710 ◽  
Author(s):  
Elisabetta Boeri Erba ◽  
Brandon T. Ruotolo ◽  
Daniel Barsky ◽  
Carol V. Robinson
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry ◽  
Multiprotein Complexes

Ion mobility-mass spectrometry of charge-reduced protein complexes reveals general trends in the collisional ejection of compact subunits

The Analyst ◽  
2015 ◽  
Vol 140 (20) ◽  
pp. 7020-7029 ◽  
Author(s):  
Russell E. Bornschein ◽  
Brandon T. Ruotolo
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Total Protein ◽  
Quaternary Structure ◽  
Protein Complexes ◽  
Complex Mixtures ◽  
Ion Mobility Mass Spectrometry ◽  
Cellular Functions ◽  
Multiprotein Complexes ◽  
Wide Range

Multiprotein complexes have been shown to play critical roles across a wide range of cellular functions, but most probes of protein quaternary structure are limited in their ability to analyze complex mixtures and polydisperse structures using small amounts of total protein.

scholarly journals Integrating Ion Mobility Mass Spectrometry with Molecular Modelling to Determine the Architecture of Multiprotein Complexes

PLoS ONE ◽  
2010 ◽  
Vol 5 (8) ◽  
pp. e12080 ◽  
Author(s):  
Argyris Politis ◽  
Ah Young Park ◽  
Suk-Joon Hyung ◽  
Daniel Barsky ◽  
Brandon T. Ruotolo ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Molecular Modelling ◽  
Ion Mobility Mass Spectrometry ◽  
Multiprotein Complexes

Rapid Diagnosis of Parkinson’s Disease from Sebum using Paper Spray Ionisation Ion Mobility Mass Spectrometry

2020 ◽  
Author(s):  
Depanjan Sarkar ◽  
Drupad Trivedi ◽  
Eleanor Sinclair ◽  
Sze Hway Lim ◽  
Caitlin Walton-Doyle ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Ion Mobility ◽  
Neurodegenerative Disorder ◽  
Treatment Options ◽  
Ion Mobility Mass Spectrometry ◽  
Paper Spray ◽  
Molecular Features ◽  
Validation Set

Parkinson’s disease (PD) is the second most common neurodegenerative disorder for which identification of robust biomarkers to complement clinical PD diagnosis would accelerate treatment options and help to stratify disease progression. Here we demonstrate the use of paper spray ionisation coupled with ion mobility mass spectrometry (PSI IM-MS) to determine diagnostic molecular features of PD in sebum. PSI IM-MS was performed directly from skin swabs, collected from 34 people with PD and 30 matched control subjects as a training set and a further 91 samples from 5 different collection sites as a validation set. PSI IM-MS elucidates ~ 4200 features from each individual and we report two classes of lipids (namely phosphatidylcholine and cardiolipin) that differ significantly in the sebum of people with PD. Putative metabolite annotations are obtained using tandem mass spectrometry experiments combined with accurate mass measurements. Sample preparation and PSI IM-MS analysis and diagnosis can be performed ~5 minutes per sample offering a new route to for rapid and inexpensive confirmatory diagnosis of this disease.

P‐97: Separation of Isomeric OLED Materials Using Ion Mobility‐Mass Spectrometry (IM‐MS)

2021 ◽  
Vol 52 (1) ◽  
pp. 1444-1447
Author(s):  
Hirotaka Shioji ◽  
Azusa Uematsu ◽  
Motoshi Onoda ◽  
Keiko Matsuda ◽  
Keisuke Sawada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Ion Mobility Mass Spectrometry
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