scholarly journals Tandem Laser-Induced Fluorescence and Mass Spectrometry Detection for High-Performance Liquid Chromatography Analysis of the in Vitro Metabolism of Doxorubicin

2010 ◽  
Vol 82 (19) ◽  
pp. 8113-8120 ◽  
Author(s):  
Joseph B. Katzenmeyer ◽  
Christopher V. Eddy ◽  
Edgar A. Arriaga
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Laser Induced Fluorescence ◽  
In Vitro Metabolism ◽  
Mass Spectrometry Detection ◽  
Chromatography Analysis ◽  
Performance Liquid Chromatography Analysis

Quantitative high-performance liquid chromatography analysis of DNA oxidized in vitro and in vivo

1991 ◽  
Vol 196 (1) ◽  
pp. 126-136 ◽  
Author(s):  
Krystyna Frenkel ◽  
Zhaojing Zhong ◽  
Huachen Wei ◽  
Jerzy Karkoszka ◽  
Umesh Patel ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Chromatography Analysis ◽  
Performance Liquid Chromatography Analysis

scholarly journals Inhibition of Polyamine Synthesis Arrests Trichomonad Growth and Induces Destruction of Hydrogenosomes

1999 ◽  
Vol 43 (8) ◽  
pp. 1919-1923 ◽  
Author(s):  
Isabela A. Reis ◽  
Martha P. Martinez ◽  
Nigel Yarlett ◽  
Patricia J. Johnson ◽  
Fernando C. Silva-Filho ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Protective Role ◽  
Tritrichomonas Foetus ◽  
Polyamine Synthesis ◽  
Stabilizing Agents ◽  
Chromatography Analysis ◽  
Performance Liquid Chromatography Analysis

ABSTRACT Trichomonad parasites such as Tritrichomonas foetusproduce large amounts of putrescine (1,4-diaminobutane), which is transported out of the cell via an antiport mechanism which results in the uptake of a molecule of spermine. The importance of putrescine to the survival of the parasite and its role in the biology of T. foetus was investigated by use of the putrescine analogue 1,4-diamino-2-butanone (DAB). Growth of T. foetus in vitro was significantly inhibited by 20 mM DAB, which was reversed by the addition of exogenous 40 mM putrescine. High-performance liquid chromatography analysis of 20 mM DAB-treated T. foetusrevealed that putrescine, spermidine, and spermine levels were reduced by 89, 52, and 43%, respectively, compared to those in control cells. The DAB treatment induced several ultrastructural alterations, which were primarily observed in the redox organelles termed hydrogenosomes. These organelles were progressively degraded, giving rise to large vesicles that displayed material immunoreactive with an antibody to β-succinyl-coenzyme A synthetase, a hydrogenosomal enzyme. A protective role for polyamines as stabilizing agents in the trichomonad hydrogenosomal membrane is proposed.

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