Solid Phase Isobaric Mass Tag Reagent for Simultaneous Protein Identification and Assay

2010 ◽  
Vol 82 (13) ◽  
pp. 5552-5560 ◽  
Author(s):  
Anna Napoli ◽  
Constantinos M. Athanassopoulos ◽  
Petros Moschidis ◽  
Donatella Aiello ◽  
Leonardo Di Donna ◽  
...  
Keyword(s):  
Protein Identification ◽  
Solid Phase ◽  
Mass Tag

Design and synthesis of a solid-phase fluorescent mass tag

2005 ◽  
Vol 28 (14) ◽  
pp. 1812-1817 ◽  
Author(s):  
Yang Shi ◽  
Rong Xiang ◽  
Csaba Horváth ◽  
James A. Wilkins
Keyword(s):  
Solid Phase ◽  
Design And Synthesis ◽  
Mass Tag

Delayed Fragmentation and Optimized Isolation Width Settings for Improvement of Protein Identification and Accuracy of Isobaric Mass Tag Quantification on Orbitrap-Type Mass Spectrometers

2011 ◽  
Vol 83 (23) ◽  
pp. 8959-8967 ◽  
Author(s):  
Mikhail M. Savitski ◽  
Gavain Sweetman ◽  
Manor Askenazi ◽  
Jarrod A. Marto ◽  
Manja Lang ◽  
...  
Keyword(s):  
Protein Identification ◽  
Mass Spectrometers ◽  
Type Mass ◽  
Mass Tag

scholarly journals Comparison of Traditional and Industrial Sausages - Baranjski Kulen and Kulenova Seka Using Comprehensive Proteome, Peptidome and Metabolome Techniques

2022 ◽  
Vol 60 (2) ◽  
Author(s):  
Valerija Šimunec ◽  
Rea Bertoša ◽  
Anita Šporec ◽  
Igor Lukić ◽  
Diana Nejašmić ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Amino Acids ◽  
Volatile Compounds ◽  
Industrial Production ◽  
Free Amino Acids ◽  
Free Amino ◽  
Protein Identification ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Fermented Sausage

Research background. Baranjski kulen is one of the most popular fermented meat sausages originating from Croatia. It has protected geographical indication, and is traditionally produced in the Baranja region of Croatia. Kulenova seka is a fermented sausage very similar to Baranjski kulen, but it has a different caliber and consequently, a shorter time of production. In recent decades, due to the high demand and popularity of these products, industrially produced Baranjski kulen and Kulenova seka have become available on the market. This work aims to identify specific characteristics of traditional and industrial sausages, Baranjski kulen and Kulenova seka on proteome, peptidome and metabolome level which could potentially lead to better optimization of the industrial production process in order to obtain an equivalent to the traditional product. Experimental approach. Protein profiles of Baranjski kulen and Kulenova seka (traditional and industrial) were analysed using two-dimensional gel electrophoresis followed by differential display analysis and protein identification using mass spectrometry. Peptidomics profiling analysis was performed via liquid chromatography-tandem mass spectrometry. Furthermore, aroma profiles were investigated via headspace solid phase microextraction and gas chromatography-mass spectrometry. Results and conclusions. The major identified characteristics of each product were: industrial Baranjski kulen - specific degradation of MYH1 and TITIN, overabundance of stress-related proteins and increased phenylalanine degradation; traditional Baranjski kulen - decreased concentration of phenylalanine and overabundance of ALDOA and CAH3; industrial Kulenova seka - specific MYH4 and HBA degradation process; traditional Kulenova seka - overabundance of DPYD and MYL1, degradation of ALBU and MYG, decreased concentrations of almost all free amino acids and increased amounts of smoke derived volatile compounds. Novelty and scientific contribution. In this preliminary communication, the first insights into protein degradation processes and generation of peptides, free amino acids and aroma compounds of industrial and traditional Baranjski kulen and Kulenova seka are presented. Although further research is needed to draw general conclusions, the specific profile of proteins, peptides, amino acids, and volatile compounds represents the first step in the industrial production of sausages that meet the characteristics of traditional flavour.

scholarly journals Combined Use of a Solid-Phase Hexapeptide Ligand Library with Liquid Chromatography and Two-Dimensional Difference Gel Electrophoresis for Intact Plasma Proteomics

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Tatsuo Hagiwara ◽  
Yumi Saito ◽  
Yukiko Nakamura ◽  
Takeshi Tomonaga ◽  
Yasufumi Murakami ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Plasma Proteins ◽  
Large Scale ◽  
Protein Identification ◽  
Solid Phase ◽  
Mass Spectrometric ◽  
Two Dimensional ◽  
Anion Exchange Column ◽  
Difference Gel Electrophoresis ◽  
Plasma Proteomics

The intact plasma proteome is of great interest in biomarker studies because intact proteins reflect posttranslational protein processing such as phosphorylation that may correspond to disease status. We examined the utility of a solid-phase hexapeptide ligand library in combination with conventional plasma proteomics modalities for comprehensive profiling of intact plasma proteins. Plasma proteins were sequentially fractionated using depletion columns for albumin and immunoglobulin, and separated using an anion-exchange column. Proteins in each fraction were treated with a solid-phase hexapeptide ligand library and compared to those without treatment. Two-dimensional difference gel electrophoresis demonstrated an increased number of protein spots in the treated samples. Mass spectrometric studies of these protein spots with unique intensity in the treated samples resulted in the identification of high- and medium-abundance proteins. Our results demonstrated the possible utility of a solid-phase hexapeptide ligand library to reveal greater number of intact plasma proteins. The characteristics of proteins with unique affinity to the library remain to be clarified by more extensive mass spectrometric protein identification, and optimized protocols should be established for large-scale plasma biomarker studies.

Solid-phase immunoelectron microscopy for rapid diagnosis of enteroviruses

1984 ◽  
Vol 42 ◽  
pp. 226-227 ◽  
Author(s):  
K. Pegg-Feige ◽  
F. W. Doane
Keyword(s):  
Electron Microscopy ◽  
Cell Culture ◽  
Immunoelectron Microscopy ◽  
Detection Method ◽  
Solid Phase ◽  
Protein A ◽  
Plant Viruses ◽  
Rapid Diagnosis ◽  
Virus Diagnosis ◽  
Antiviral Antibody

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.

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