scholarly journals Particle Focusing in Staged Inertial Microfluidic Devices for Flow Cytometry

2010 ◽  
Vol 82 (9) ◽  
pp. 3862-3867 ◽  
Author(s):  
John Oakey ◽  
Robert W. Applegate ◽  
Erik Arellano ◽  
Dino Di Carlo ◽  
Steven W. Graves ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Devices ◽  
Particle Focusing

Inertial particle focusing and spacing control in microfluidic devices

2018 ◽  
Vol 22 (3) ◽  
Author(s):  
Chao Wang ◽  
Sifan Sun ◽  
Ying Chen ◽  
Zhengdong Cheng ◽  
Yuxiu Li ◽  
...  
Keyword(s):  
Microfluidic Devices ◽  
Inertial Particle ◽  
Particle Focusing

Concentration-controlled particle focusing in spiral elasto-inertial microfluidic devices

2017 ◽  
Vol 39 (2) ◽  
pp. 417-424 ◽  
Author(s):  
Nan Xiang ◽  
Zhonghua Ni ◽  
Hong Yi
Keyword(s):  
Microfluidic Devices ◽  
Particle Focusing

Flow Cytometry, Beads, and Microchannels

2005 ◽  
Author(s):  
Tione Buranda ◽  
Larry A. Sklar
Keyword(s):  
Flow Cytometry ◽  
Dna Analysis ◽  
Microfluidic Devices ◽  
Model Systems ◽  
Limiting Factor ◽  
Flat Surfaces ◽  
Combined Use ◽  
Potential Applications ◽  
Small Reynolds Numbers ◽  
Flow Through

Microfluidic devices generally consume microliter to submicroliter volumes of sample and are thus well suited for use when the required reagents are scarce or expensive. Because microfluidic devices operate in a regime in which small Reynolds numbers govern the delivery of fluid samples, reagent mixing and subsequent reactivity has been a severe limiting factor in their applicability. The inclusion of packed beads in the microfluidic device repertoire has several advantages: molecular assemblies for the assay are created outside the channel on beads and characterized with flow cytometry, uniform populations of beads may be assured through rapid cytometric sorting, beads present a larger surface area for the display of receptors than flat surfaces, rapid mixing in the microcolumn is achieved because the distance that must be covered by diffusion is limited to the (≤1-μm) interstitial space between the closely packed receptorbearing beads, and analytes are captured in a flow-through format and, as such, each bead can act as a local concentrator of analytes. Progress in the combined use of beads and microfluidic devices has been limited by the ability to pack beads at specific sections of microfluidic devices. A subsequent challenge associated with the packed microcolumns of beads is the substantial pressure drop that affects the fluid flow velocity. However, some of these challenges have been overcome in the design of simple model systems that have potential applications in DNA analysis, chromatography, and immunoassays. It is the intent of this chapter to examine the recent emergence of small-volume heterogeneous immunoassays, using beads trapped in microchannels, while excluding other closely related applications such as capillary electrophoresis and flow injection–based approaches. Of necessity, the authors’ interests and availability of information pertinent to the specific discussions presented below impose additional restrictions. To date, there are only a handful of applications that combine packed beads and microfluidic devices, and even fewer that make the overt connection between flow cytometry–based assays and beads. Harrison and coworkers have provided one of the earliest conceptual demonstrations of the capability to incorporate packed beads in microfluidic devices for analytical purposes.

Particle focusing in microfluidic devices

2010 ◽  
Vol 9 (1) ◽  
pp. 1-16 ◽  
Author(s):  
Xiangchun Xuan ◽  
Junjie Zhu ◽  
Christopher Church
Keyword(s):  
Microfluidic Devices ◽  
Particle Focusing

scholarly journals Flow Cytometry on Microfluidic Devices

2000 ◽  
Author(s):  
S.C. Jacobson ◽  
M.A. McClain ◽  
C.T. Culbertson ◽  
J.M. Ramsey
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Devices

Sheath Free Three-Dimensional Particle Focusing Using Electro-Hydrodynamics

2008 ◽  
Author(s):  
Young Won Kim ◽  
Jung Yul Yoo
Keyword(s):  
Flow Cytometry ◽  
Three Dimensional ◽  
Particle Beam ◽  
Optical Systems ◽  
Beam Diameter ◽  
Particle Focusing ◽  
Rigid Particles ◽  
Mems Technology ◽  
Micro Flow ◽  
Key Techniques

A conventional flow cytometry is a crucial and versatile instrument that measures the fluorescence and light scattering of individual cell and other particulate or molecular analytes in biomedical research. Recently, there have been many efforts to achieve this conventional flow cytometry on lab-on-a-chip devices powered by microfluidics. One of the key techniques of this instrument is to hydrodynamically focus samples within the sensing area of optical systems in order to optimize detection signals. Recent three-dimensional focusing methods using MEMS technology contain sheath flows with very complex channel geometries. In the present investigation, we demonstrated 3-D particle focusing in a single straight micro-capillary without sheath flows using electro-hydrodynamics. Particle beam was successfully made with a beam diameter of about 10 μm for rigid particles. Proposed device provides critical solutions for simple and innovative 3-D particle focusing method for the applications to the MEMS-based micro-flow cytometry.

Flow Cytometry ofEscherichia colion Microfluidic Devices

2001 ◽  
Vol 73 (21) ◽  
pp. 5334-5338 ◽  
Author(s):  
Maxine A. McClain ◽  
Christopher T. Culbertson ◽  
Stephen C. Jacobson ◽  
J. Michael Ramsey
Keyword(s):  
Flow Cytometry ◽  
Microfluidic Devices
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