Electrical Sensor Array for Polymerase Chain Reaction-Free Messenger RNA Expression Profiling

2010 ◽  
Vol 82 (14) ◽  
pp. 5958-5964 ◽  
Author(s):  
Xiaojun Chen ◽  
Somenath Roy ◽  
Yanfen Peng ◽  
Zhiqiang Gao
Keyword(s):  
Polymerase Chain Reaction ◽  
Expression Profiling ◽  
Messenger Rna ◽  
Sensor Array ◽  
Rna Expression ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Rna Expression Profiling

scholarly journals Altered expression of small leucine-rich proteoglycans (Decorin, Biglycan and Lumican): Plausible diagnostic marker in urothelial carcinoma of bladder

Tumor Biology ◽  
2017 ◽  
Vol 39 (5) ◽  
pp. 101042831769911 ◽  
Author(s):  
Sandeep Appunni ◽  
Vivek Anand ◽  
Madhuram Khandelwal ◽  
Amlesh Seth ◽  
Sandeep Mathur ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Bladder Cancer ◽  
Urothelial Carcinoma ◽  
Messenger Rna ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Expression ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Lower Expression ◽  
Urothelial Carcinoma Of Bladder

Small leucine-rich proteoglycans are components of extracellular matrix that regulates neoplastic transformation. Among small leucine rich proteoglycans, Decorin, Biglycan and Lumican are most commonly implicated markers, and their expression is well studied in various malignancies. In this novel study, we have collectively evaluated expression of these three molecules in urothelial carcinoma of bladder. Thirty patients of confirmed untreated bladder cancer, 30 healthy controls for blood and 30 controls for adjacent non-tumour tissue were enrolled. Blood was collected from all subjects and tumour/adjacent normal tissue was obtained from the patients. Circulatory levels were estimated by enzyme-linked immunosorbent assay, relative messenger RNA expression by quantitative polymerase chain reaction and protein expression by immunohistochemistry and western-blotting. Circulatory levels of Biglycan (p = 0.0038) and Lumican (p < 0.0001) were significantly elevated, and that of Decorin (p < 0.0001) was significantly reduced in patients as compared with controls. Protein expression by immunohistochemistry and western-blotting showed elevated expression of Lumican and Biglycan and lower expression of Decorin in urothelial carcinoma of bladder. Quantitative polymerase chain reaction for messenger RNA expression from tissue specimens revealed significantly higher expression of Biglycan (p = 0.0008) and Lumican (p = 0.01) and lower expression of Decorin (p < 0.0001) in urothelial carcinoma of bladder. Out of all molecules receiver operating characteristic curve showed that the 0.207 ng/ml cut-off of serum Lumican provided optimum sensitivity (90.0%) and specificity (90.0%). Significant alteration of matrix small leucine-rich proteoglycans in urothelial carcinoma of bladder was observed. Higher expression of Lumican in Bladder cancer patients with the cut-off value of highest optimum sensitivity and specificity shows its importance as a potential non-invasive marker for early detection of UBC following further validation in large patient cohort.

Quantitative Polymerase Chain Reaction (PCR) to Analyze Messenger-RNA Expression in Tissue Obtained by Directional Atherectomy

Angiology ◽  
1994 ◽  
Vol 45 (11) ◽  
pp. 911-916
Author(s):  
Ralf Köster ◽  
Ulrich Windstetter ◽  
James M. Gulizia ◽  
Peter Uberfuhr ◽  
Günther Baumann ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
Quantitative Polymerase Chain Reaction ◽  
Rna Expression ◽  
Chain Reaction ◽  
Directional Atherectomy ◽  
Polymerase Chain

High performance Nanogold™-in situ hybridzation and its use in the detection of hybridized and PCR-amplified microscopical preparations

1996 ◽  
Vol 54 ◽  
pp. 896-897
Author(s):  
G. W. Hacker ◽  
I. Zehbe ◽  
J. Hainfeld ◽  
A.-H. Graf ◽  
C. Hauser-Kronberger ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Messenger Rna ◽  
High Performance ◽  
Detection System ◽  
Direct Methods ◽  
Genetic Alterations ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Polymerase Chain

In situ hybridization (ISH) with biotin-labeled probes is increasingly used in histology, histopathology and molecular biology, to detect genetic nucleic acid sequences of interest, such as viruses, genetic alterations and peptide-/protein-encoding messenger RNA (mRNA). In situ polymerase chain reaction (PCR) (PCR in situ hybridization = PISH) and the new in situ self-sustained sequence replication-based amplification (3SR) method even allow the detection of single copies of DNA or RNA in cytological and histological material. However, there is a number of considerable problems with the in situ PCR methods available today: False positives due to mis-priming of DNA breakdown products contained in several types of cells causing non-specific incorporation of label in direct methods, and re-diffusion artefacts of amplicons into previously negative cells have been observed. To avoid these problems, super-sensitive ISH procedures can be used, and it is well known that the sensitivity and outcome of these methods partially depend on the detection system used.

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