scholarly journals Measurement of Human Surfactant Protein-B Turnover in Vivo from Tracheal Aspirates Using Targeted Proteomics

2010 ◽  
Vol 82 (6) ◽  
pp. 2561-2567 ◽  
Author(s):  
Daniela M. Tomazela ◽  
Bruce W. Patterson ◽  
Elizabeth Hanson ◽  
Kimberly L. Spence ◽  
Tiffany B. Kanion ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Targeted Proteomics ◽  
Surfactant Protein B ◽  
Tracheal Aspirates ◽  
Protein B

scholarly journals Allele-specific N-glycosylation delays human surfactant protein B secretion in vitro and associates with decreased protein levels in vivo

2013 ◽  
Vol 74 (6) ◽  
pp. 646-651 ◽  
Author(s):  
Saija Taponen ◽  
Johanna M. Huusko ◽  
Ulla E. Petäjä-Repo ◽  
Reija Paananen ◽  
Susan H. Guttentag ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Protein Levels ◽  
Surfactant Protein B ◽  
Allele Specific ◽  
Protein B

Clearance of surfactant protein B from rabbit lungs

1995 ◽  
Vol 268 (4) ◽  
pp. L636-L641 ◽  
Author(s):  
T. Ueda ◽  
M. Ikegami ◽  
M. Henry ◽  
A. H. Jobe
Keyword(s):  
Half Life ◽  
Lamellar Body ◽  
Surfactant Protein ◽  
Adult Rabbit ◽  
Lamellar Bodies ◽  
Surfactant Protein B ◽  
Clearance Kinetics ◽  
Protein B

To characterize the metabolism of surfactant protein B (SP-B) in vivo, we measured the clearance of SP-B from adult rabbit lungs. Purified rabbit SP-B was radiolabeled with 125I by the Bolton-Hunter method. Trace amounts of 125I-labeled SP-B mixed with [14C]dipalmitoylphosphatidylcholine (DPPC) were given intratracheally via a bronchoscope to rabbits 0-16 h before collection of alveolar washes (AW). Macrophages were recovered from AW, and lamellar bodies (LB) were isolated from lung tissue by differential centrifugation. 125I-SP-B was cleared more rapidly from the airspaces and the total lung (half-life 7 h) than was DPPC (half-life 11 h in the total lung). There was an approximately threefold accumulation of SP-B relative to saturated phosphatidylcholine in macrophages at all times. The proportion of 125I and 14C radioactivities in lamellar bodies was similar at 2 and 4 h, but there was 14-fold less 125I-SP-B than [14C]DPPC in lamellar bodies by 16 h. This loss of SP-B from the lamellar body fraction is consistent with less recycling of SP-B. The results demonstrate different clearance kinetics of these two components of surfactant and indicate a significant role of macrophages in the clearance of SP-B.

scholarly journals Developmental and Genetic Regulation of Human Surfactant Protein B in vivo

Neonatology ◽  
2009 ◽  
Vol 95 (2) ◽  
pp. 117-124 ◽  
Author(s):  
Aaron Hamvas ◽  
Hillary B. Heins ◽  
Susan H. Guttentag ◽  
Daniel J. Wegner ◽  
Michelle A. Trusgnich ◽  
...  
Keyword(s):  
Genetic Regulation ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Surfactant Protein-B Supplementation Improves In Vivo Function of a Modified Natural Surfactant

1995 ◽  
Vol 37 (3) ◽  
pp. 271-276 ◽  
Author(s):  
Katsumi Mizuno ◽  
Machiko Ikegami ◽  
Chung-Ming Chen ◽  
Takashi Ueda ◽  
Alan H Jobe
Keyword(s):  
Surfactant Protein ◽  
Natural Surfactant ◽  
Surfactant Protein B ◽  
Protein B

The pathogenic role of surfactant protein B in formation of bronchopulmonary pathology in children

2017 ◽  
pp. 66-71
Author(s):  
O. Yablon ◽  
◽  
N. Zaichko ◽  
O. Mazulov ◽  
Z.I. Rossokha ◽  
...  
Keyword(s):  
Surfactant Protein ◽  
Pathogenic Role ◽  
Surfactant Protein B ◽  
Protein B

scholarly journals Fetal and neonatal samples of a precursor surfactant protein B inversely related to gestational age

2013 ◽  
Vol 13 (1) ◽  
Author(s):  
Christoph Czernik ◽  
Gerd Schmalisch ◽  
Christoph Bührer ◽  
Hans Proquitté
Keyword(s):  
Gestational Age ◽  
Surfactant Protein ◽  
Surfactant Protein B ◽  
Protein B
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