scholarly journals Ionic Liquid Matrix for Direct UV-MALDI-TOF-MS Analysis of Dermatan Sulfate and Chondroitin Sulfate Oligosaccharides

2007 ◽  
Vol 79 (4) ◽  
pp. 1604-1610 ◽  
Author(s):  
Tatiana N. Laremore ◽  
Fuming Zhang ◽  
Robert J. Linhardt
Keyword(s):  
Ionic Liquid ◽  
Chondroitin Sulfate ◽  
Dermatan Sulfate ◽  
Liquid Matrix ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

scholarly journals Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Hoa Thi Le ◽  
Kyu H. Park ◽  
Woong Jung ◽  
Hyung Soon Park ◽  
Tae Woo Kim
Keyword(s):  
Ionic Liquid ◽  
Human Serum ◽  
Solid Phase ◽  
Liquid Matrix ◽  
Dihydroxybenzoic Acid ◽  
Established Method ◽  
Maldi Tof Ms ◽  
Microwave Assisted ◽  
Maldi Tof ◽  
Tof Ms

We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS. The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range. Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans. However, with the established method, i.e., DHB/An method, only 15 glycans were assigned. To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.

Ionic liquid matrices for MALDI-TOF-MS analysis of intact glycoproteins

2010 ◽  
Vol 398 (1) ◽  
pp. 357-365 ◽  
Author(s):  
Estela Giménez ◽  
Fernando Benavente ◽  
José Barbosa ◽  
Victoria Sanz-Nebot
Keyword(s):  
Ionic Liquid ◽  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Liquid Matrices ◽  
Ms Analysis ◽  
Ionic Liquid Matrices ◽  
Tof Ms

The suitability of different DHB isomers as matrices for the MALDI-TOF MS analysis of phospholipids: which isomer for what purpose?

2006 ◽  
Vol 36 (4-5) ◽  
pp. 517-527 ◽  
Author(s):  
Jürgen Schiller ◽  
Rosmarie Süß ◽  
Beate Fuchs ◽  
Matthias Müller ◽  
Marijana Petković ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

NMR, HPLC-ESI-MS, and MALDI-TOF MS Analysis of Condensed Tannins from Delonix regia (Bojer ex Hook.) Raf. and Their Bioactivities

2012 ◽  
Vol 60 (19) ◽  
pp. 5013-5022 ◽  
Author(s):  
Wei-Ming Chai ◽  
Yan Shi ◽  
Hui-Ling Feng ◽  
Ling Qiu ◽  
Hai-Chao Zhou ◽  
...  
Keyword(s):  
Condensed Tannins ◽  
Maldi Tof Ms ◽  
Esi Ms ◽  
Delonix Regia ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Tof Ms

Identification of Lice Stored in Alcohol Using MALDI-TOF MS

2020 ◽  
Author(s):  
Hanene Benyahia ◽  
Basma Ouarti ◽  
Adama Zan Diarra ◽  
Mehdi Boucheikhchoukh ◽  
Mohamed Nadir Meguini ◽  
...  
Keyword(s):  
Economic Consequences ◽  
Correct Identification ◽  
Protein Profiles ◽  
Innovative Strategy ◽  
Maldi Tof Ms ◽  
Pediculus Humanus ◽  
Maldi Tof ◽  
Ms Analysis ◽  
Storage Methods ◽  
Tof Ms

Abstract Lice pose major public and veterinary health problems with economic consequences. Their identification is essential and requires the development of an innovative strategy. MALDI-TOF MS has recently been proposed as a quick, inexpensive, and accurate tool for the identification of arthropods. Alcohol is one of the most frequently used storage methods and makes it possible to store samples for long periods at room temperature. Several recent studies have reported that alcohol alters protein profiles resulting from MS analysis. After preliminary studies on frozen lice, the purpose of this research was to evaluate the influence of alcohol preservation on the accuracy of lice identification by MALDI-TOF MS. To this end, lice stored in alcohol for variable periods were submitted for MS analysis and sample preparation protocols were optimized. The reproducibility and specificity of the MS spectra obtained on both these arthropod families allowed us to implement the reference MS spectra database (DB) with protein profiles of seven lice species stored in alcohol. Blind tests revealed a correct identification of 93.9% of Pediculus humanus corporis (Linnaeus, 1758) and 98.4% of the other lice species collected in the field. This study demonstrated that MALDI-TOF MS could be successfully used for the identification of lice stored in alcohol for different lengths of time.

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