Membrane Proteome Analysis of Microdissected Ovarian Tumor Tissues Using Capillary Isoelectric Focusing/Reversed-Phase Liquid Chromatography−Tandem MS

2007 ◽  
Vol 79 (3) ◽  
pp. 1002-1009 ◽  
Author(s):  
Weijie Wang ◽  
Tong Guo ◽  
Paul A. Rudnick ◽  
Tao Song ◽  
Jie Li ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
Ovarian Tumor ◽  
Proteome Analysis ◽  
Reversed Phase ◽  
Tandem Ms ◽  
Reversed Phase Liquid Chromatography ◽  
Membrane Proteome ◽  
Tumor Tissues ◽  
Phase Liquid

Isoelectric Focusing-Reversed Phase Liquid Chromatography Polymer Microchip With Integrated High-Pressure Valves

2009 ◽  
Author(s):  
Jikun Liu ◽  
Chien-Fu Chen ◽  
Chien-Cheng Chang ◽  
Don L. DeVoe
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Isoelectric Focusing ◽  
Peptide Mass Fingerprinting ◽  
Gradient Elution ◽  
Reversed Phase ◽  
Reversed Phase Liquid Chromatography ◽  
Peptide Mass ◽  
Uniform Sample ◽  
Phase Liquid

A cyclicolefin polymer (COP) microchip supporting parallel 2-D peptide separations is described. By combining isoelectric focusing (IEF) as a first dimension and parallel reversed-phase liquid chromatography (RPLC) as a second dimension, the system enables efficient high-throughput fractionation prior to mass spectrometry in support of peptide mass fingerprinting for global proteomic analysis from highly limited specimens. The IEF-RPLC chip incorporates high-pressure micro shut-off valves, allowing uniform sample transfer and gradient elution from each micro LC column, and ensuring hydrodynamic isolation between the separation dimensions. The utility of the initial microchip is demonstrated by separation of a fluorescein labeled bovine serum albumin tryptic digest in a chip containing a five channel RPLC array.

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