Label-Free Protein Biosensor Based on Aptamer-Modified Carbon Nanotube Field-Effect Transistors

2007 ◽  
Vol 79 (2) ◽  
pp. 782-787 ◽  
Author(s):  
Kenzo Maehashi ◽  
Taiji Katsura ◽  
Kagan Kerman ◽  
Yuzuru Takamura ◽  
Kazuhiko Matsumoto ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free ◽  
Free Protein

Label-free protein detection based on vertically aligned carbon nanotube gated field-effect transistors

2011 ◽  
Vol 160 (1) ◽  
pp. 154-160 ◽  
Author(s):  
Robert A. Croce Jr ◽  
Sagar Vaddiraju ◽  
Pik-Yiu Chan ◽  
Rea Seyta ◽  
Faquir C. Jain
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Protein Detection ◽  
Label Free ◽  
Free Protein ◽  
Vertically Aligned

Aptamer-Based Label-Free Immunosensors Using Carbon Nanotube Field-Effect Transistors

2009 ◽  
Vol 21 (11) ◽  
pp. 1285-1290 ◽  
Author(s):  
Kenzo Maehashi ◽  
Kazuhiko Matsumoto ◽  
Yuzuru Takamura ◽  
Eiichi Tamiya
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free

Detection of Tumor Markers Using Single-Walled Carbon Nanotube Field Effect Transistors

2006 ◽  
Vol 6 (11) ◽  
pp. 3499-3502 ◽  
Author(s):  
Dong-Won Park ◽  
Yo-Han Kim ◽  
Beom Soo Kim ◽  
Hye-Mi So ◽  
Keehoon Won ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Tumor Markers ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Sharp Decrease ◽  
Tween 20 ◽  
Label Free ◽  
Single Walled Carbon Nanotube ◽  
Single Walled Carbon ◽  
Specific Tumor

We have developed a biosensor capable of detecting carcinoembryonic antigen (CEA) markers using single-walled carbon nanotube field effect transistors (SWNT-FETs). These SWNT-FETs were fabricated using nanotubes produced by a patterned catalyst growth technique, where the top contact electrodes were generated using conventional photolithography. For biosensor applications, SU-8 negative photoresist patterns were used as an insulation layer. CEA antibodies were employed as recognition elements to specific tumor markers, and were successfully immobilized on the sides of a single-walled carbon nanotube using CDI-Tween 20 linking molecules. The binding of tumor markers to these antibody-functionalized SWNT-FETs was then monitored continuously during exposure to dilute CEA solutions. The observed sharp decrease in conductance demonstrates the possibility of realizing highly sensitive, label-free SWNT-FET-based tumor sensors.

Label-Free DNA Biosensors Based on Functionalized Carbon Nanotube Field Effect Transistors

Nano Letters ◽  
2009 ◽  
Vol 9 (2) ◽  
pp. 530-536 ◽  
Author(s):  
Maria Teresa Martínez ◽  
Yu-Chih Tseng ◽  
Nerea Ormategui ◽  
Iraida Loinaz ◽  
Ramon Eritja ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free ◽  
Dna Biosensors ◽  
Free Dna ◽  
Functionalized Carbon Nanotube

Self-assembled nanotube field-effect transistors for label-free protein biosensors

2008 ◽  
Vol 104 (7) ◽  
pp. 074310 ◽  
Author(s):  
P. Hu ◽  
A. Fasoli ◽  
J. Park ◽  
Y. Choi ◽  
P. Estrela ◽  
...  
Keyword(s):  
Field Effect ◽  
Field Effect Transistors ◽  
Label Free ◽  
Free Protein ◽  
Self Assembled

scholarly journals Effect of channel length on the electrical response of carbon nanotube field-effect transistors to deoxyribonucleic acid hybridization

2014 ◽  
Vol 5 ◽  
pp. 2081-2091 ◽  
Author(s):  
Hari Krishna Salila Vijayalal Mohan ◽  
Jianing An ◽  
Yani Zhang ◽  
Chee How Wong ◽  
Lianxi Zheng
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Field Effect Transistors ◽  
Electrical Response ◽  
Channel Length ◽  
Nucleic Acid Hybridization ◽  
Label Free ◽  
Single Walled Carbon Nanotube ◽  
Label Free Detection ◽  
Simultaneous Influence

A single-walled carbon nanotube (SWCNT) in a field-effect transistor (FET) configuration provides an ideal electronic path for label-free detection of nucleic acid hybridization. The simultaneous influence of more than one response mechanism in hybridization detection causes a variation in electrical parameters such as conductance, transconductance, threshold voltage and hysteresis gap. The channel length (L) dependence of each of these parameters necessitates the need to include them when interpreting the effect of L on the response to hybridization. Using the definitions of intrinsic effective mobility (µe) and device field-effect mobility (µf), two new parameters were defined to interpret the effect of L on the FET response to hybridization. Our results indicate that FETs with ≈300 µm long SWCNT exhibited the most appreciable response to hybridization, which complied with the variation trend in response to the newly defined parameters.

LABEL-FREE BIOMOLECULAR DETECTION USING CARBON NANOTUBE FIELD EFFECT TRANSISTORS

NANO ◽  
2008 ◽  
Vol 03 (06) ◽  
pp. 415-431 ◽  
Author(s):  
HYE RYUNG BYON ◽  
SUPHIL KIM ◽  
HEE CHEUL CHOI
Keyword(s):  
Carbon Nanotube ◽  
Field Effect ◽  
Surface Passivation ◽  
Field Effect Transistors ◽  
High Sensitivity ◽  
Detection Methods ◽  
Label Free ◽  
Biomolecular Detection ◽  
Great Opportunity ◽  
High Level

Carbon nanotube field effect transistor (FET) type biosensors have been widely investigated as one of the promising platforms for highly sensitive personalized disease-monitoring electronic devices. Combined with high level cutting edge information technology (IT) infra systems, carbon nanotube transistor biosensors afford a great opportunity to contribute to human disease care by providing early diagnostic capability. Several key prerequisites that should be clarified for the real application include sensitivity, reliability, reproducibility, and expandability to multiplex detection systems. In this brief review, we introduce the types, fabrication, and detection methods of single-walled carbon nanotube FET (SWNT-FET) devices. As surface functionalization of the devices by which nonspecific bindings (NSBs) are efficiently prohibited is also another important issue regarding reliable biosensors, we discuss several key strategies about surface passivation along with examples of various biomolecules such as proteins, DNA, small molecules, aptamers, viruses, and cancer and neurodegenerative disease markers which have been successfully sensed by SWNT-FET devices. Finally, we discuss proposed detection mechanisms, according to which strategies for fabricating sensor devices having high sensitivity are determined. Two main mechanisms — charge transfer (or electrostatic gate effect) and Schottky barrier effect, depending on the place where biomolecules are adsorbed — will be covered.

scholarly journals High-Throughput Peptide Epitope Mapping Using Carbon Nanotube Field-Effect Transistors

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Steingrimur Stefansson ◽  
Martha Knight ◽  
Hena H. Kwon ◽  
Lára A. Stefansson ◽  
Saeyoung Nate Ahn
Keyword(s):  
Carbon Nanotube ◽  
Real Time ◽  
High Throughput ◽  
Field Effect ◽  
Epitope Mapping ◽  
Field Effect Transistors ◽  
Peptide Library ◽  
Label Free ◽  
Peptide Epitope ◽  
Binding Data

Label-free and real-time detection technologies can dramatically reduce the time and cost of pharmaceutical testing and development. However, to reach their full promise, these technologies need to be adaptable to high-throughput automation. To demonstrate the potential of single-walled carbon nanotube field-effect transistors (SWCNT-FETs) for high-throughput peptide-based assays, we have designed circuits arranged in an 8 × 12 (96-well) format that are accessible to standard multichannel pipettors. We performed epitope mapping of two HIV-1 gp160 antibodies using an overlapping gp160 15-mer peptide library coated onto nonfunctionalized SWCNTs. The 15-mer peptides did not require a linker to adhere to the non-functionalized SWCNTs, and binding data was obtained in real time for all 96 circuits. Despite some sequence differences in the HIV strains used to generate these antibodies and the overlapping peptide library, respectively, our results using these antibodies are in good agreement with known data, indicating that peptides immobilized onto SWCNT are accessible and that linear epitope mapping can be performed in minutes using SWCNT-FET.

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