scholarly journals Exploring Crown Ethers as Shift Reagents for Ion Mobility Spectrometry

2006 ◽  
Vol 78 (19) ◽  
pp. 6792-6800 ◽  
Author(s):  
Amy E. Hilderbrand ◽  
Sunnie Myung ◽  
David E. Clemmer
Keyword(s):  
Crown Ethers ◽  
Ion Mobility Spectrometry ◽  
Ion Mobility ◽  
Shift Reagents

scholarly journals Crown ethers as shift reagents in peptide epimer differentiation –conclusions from examination of ac-(H)FRW-NH2 petide sequences

2020 ◽  
Vol 23 (2) ◽  
pp. 177-188
Author(s):  
Magdalena M. Zimnicka
Keyword(s):  
Mass Spectrometry ◽  
Crown Ether ◽  
Crown Ethers ◽  
Ion Mobility ◽  
Ion Source ◽  
Peptide Sequence ◽  
Ion Mobility Mass Spectrometry ◽  
Peptide Molecule ◽  
Shift Reagents ◽  
Specific Association

Abstract Crown ethers with different ring sizes and substituents (18-crown-6, dibenzo-18-crown-6, dicyclohexano-18-crown-6, a chiral tetracarboxylic acid-18-crown-6 ether, dibenzo-21-crown-7, and dibenzo-30-crown-10) were evaluated as shift reagents to differentiate epimeric model peptides (tri-and tetrapeptides) using ion mobility mass spectrometry (IM-MS). The stable associates of peptide epimers with crown ethers were detected and examined using traveling-wave ion mobility time-of-flight mass spectrometer (Synapt G2-S HDMS) equipped with an electrospray ion source. The overall decrease of the epimer separation upon crown ether complexation was observed. The increase of the effectiveness of the microsolvation of a basic moiety - guanidine or ammonium group in the peptide had no or little effect on the epimer discrimination. Any increase of the epimer separation, which referred to the specific association mode between crown substituents and a given peptide sequence, was drastically reduced for the longer peptide sequence (tetrapeptide). The obtained results suggest that the application of the crown ethers as shift reagents in ion mobility mass spectrometry is limited to the formation of complexes differing in stoichiometry rather than it refers to a specific coordination mode between a crown ether and a peptide molecule.

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