LC−MS/MS Screen for Penitrem A and Roquefortine C in Serum and Urine Samples

2006 ◽  
Vol 78 (13) ◽  
pp. 4624-4629 ◽  
Author(s):  
Elizabeth R. Tor ◽  
Birgit Puschner ◽  
Michael S. Filigenzi ◽  
Asheesh K. Tiwary ◽  
Robert H. Poppenga
Keyword(s):  
Urine Samples ◽  
Roquefortine C ◽  
Serum And Urine ◽  
Serum And Urine Samples

scholarly journals Per- and polyfluoroalkyl substances in human serum and urine samples from a residentially exposed community

2017 ◽  
Vol 106 ◽  
pp. 135-143 ◽  
Author(s):  
Rachel Rogers Worley ◽  
Susan McAfee Moore ◽  
Bruce C. Tierney ◽  
Xiaoyun Ye ◽  
Antonia M. Calafat ◽  
...  
Keyword(s):  
Human Serum ◽  
Urine Samples ◽  
Polyfluoroalkyl Substances ◽  
Serum And Urine ◽  
Serum And Urine Samples

Addressing the standardisation of internal standards and preservative used in human bio fluids for NMR analysis: a method optimization

2021 ◽  
Vol 36 (3) ◽  
pp. 189-197
Author(s):  
Fatimatuzzahra’ Abd Aziz ◽  
Baharudin Ibrahim ◽  
Vikneswaran Murugaiyah ◽  
Azmi Sarriff
Keyword(s):  
Human Subjects ◽  
Internal Standard ◽  
Extended Period ◽  
Urine Samples ◽  
Nmr Analysis ◽  
Serum Samples ◽  
Healthy Human ◽  
Considerable Saving ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract Objectives A database comprising multivariate data in developing a model from nuclear magnetic resonance (NMR) analysis using human bio fluids are necessary to have reproducibility and reliability of the data. To achieve reproducibility of the data, standardised experiments, including internal standard and preservative used should be attained, especially for samples such as human bio fluids to hinder the variation among samples. The aim of the study was to optimise in commonly used human bio fluids (serum and urine) for a suitable internal standard and preservative used in extended storage samples for NMR analysis. Methods Serum and urine samples were collected from healthy human subjects. The experiment was divided into two parts, part one to evaluate 2,2,2,2-tetradeutero-4,4-dimethyl-4-silapentanoic acid (TSP) and 4,4-dimethyl-4-silapentane-1-ammonium trifluoroacetate (DSA) as the optimal internal standard for the serum and urine samples. The second part investigated the effects of preservatives in the serum and urine samples on extended storage. Results Overall, TSP and DSA are suitable to be used as an internal standard in human urine samples. However, DSA is a superior internal standard in serum samples for NMR analysis. For the effect of preservative, the results indicated that human serum and urine samples could be stored without addition of preservative in −80 °C, as no changes in NMR fingerprinting have been observed during storage in the absence or presence of the preservative. Conclusions The findings suggest the use of DSA and TSP as an internal standard in serum and urine samples, respectively. Storage of serum and urine samples without any addition of preservative for an extended period has no effect on the metabolites changes. By having a standardised method, it will offer a considerable saving in both operator and spectrometer time and most importantly produce reproducible and reliable data.

Cobalt Oxide Porous Nanocubes-Based Electrochemical Immunobiosensing of Hepatitis B Virus DNA in Blood Serum and Urine Samples

2019 ◽  
Vol 91 (9) ◽  
pp. 5824-5833 ◽  
Author(s):  
Palanisamy Kannan ◽  
Palaniappan Subramanian ◽  
Thandavarayan Maiyalagan ◽  
Zhongqing Jiang
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Serum ◽  
Cobalt Oxide ◽  
Urine Samples ◽  
Hepatitis B Virus Dna ◽  
B Virus ◽  
Serum And Urine ◽  
Serum And Urine Samples

Modification of the choriogonadotropin beta-subunit radioimmunoassay for determination of urinary choriogonadotropin.

1978 ◽  
Vol 24 (11) ◽  
pp. 1958-1961 ◽  
Author(s):  
J McCready ◽  
G D Braunstein ◽  
D Helm ◽  
M E Wade
Keyword(s):  
Pregnant Women ◽  
Radioreceptor Assay ◽  
Beta Subunit ◽  
Urine Samples ◽  
Human Choriogonadotropin ◽  
Analytical Recovery ◽  
Antibody Method ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial dilutions of urine containing choriogonadotropin by adding pooled urine from men to the standard tubes and limiting the volume of urine to 100 microliter. This modified assay has a sensitivity of 3 int. units/liter of urine and is specific for choriogonadotropin concentrations of 40 int. units/liter of urine. Analytical recovery of choriogonadotropin added to urine ranged from 96 to 105%. The within-assay CV was 7.6%; the between-assay CV was 11.8%. Concentrations of choriogonadotropin in concurrently collected serum and urine samples from pregnant women correlated well. The test can be performed within 24 h by using the double-antibody method for separating bound from free hormone, or in 3 h with a dioxane method. The assay is about 20-fold more sensitive than the 2-min or 2-h slide and tube pregnancy tests, and seven-to 12-fold more sensitive than the radioreceptor assay.

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