Selection of Smart Aptamers by Methods of Kinetic Capillary Electrophoresis

2006 ◽  
Vol 78 (9) ◽  
pp. 3171-3178 ◽  
Author(s):  
Andrei P. Drabovich ◽  
Maxim Berezovski ◽  
Victor Okhonin ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Kinetic Capillary Electrophoresis ◽  
Selection Of

A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis

The Analyst ◽  
2017 ◽  
Vol 142 (21) ◽  
pp. 4030-4038 ◽  
Author(s):  
Kazuki Hirose ◽  
Maho Tsuchida ◽  
Hinako Asakura ◽  
Koji Wakui ◽  
Keitaro Yoshimoto ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Mammalian Cells ◽  
Dna Aptamer ◽  
Dna Aptamers ◽  
Aptamer Selection ◽  
Selection For ◽  
First Time ◽  
Selection Of

A single-round DNA aptamer selection for mammalian cells was successfully achieved for the first time using a capillary electrophoresis (CE)-based methodology.

Two-peak approximation in kinetic capillary electrophoresis

The Analyst ◽  
2012 ◽  
Vol 137 (7) ◽  
pp. 1649 ◽  
Author(s):  
Leonid T. Cherney ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Kinetic Capillary Electrophoresis

scholarly journals Detection and classification of paraproteins by capillary immunofixation/subtraction

1998 ◽  
Vol 44 (4) ◽  
pp. 760-764 ◽  
Author(s):  
Xavier Bossuyt ◽  
Ann Bogaerts ◽  
Gilberte Schiettekatte ◽  
Norbert Blanckaert
Keyword(s):  
Capillary Electrophoresis ◽  
Cellulose Acetate ◽  
Cellulose Acetate Electrophoresis ◽  
Detection And Identification ◽  
Agarose Electrophoresis ◽  
Oligoclonal Banding ◽  
Capillary Zone ◽  
Electrophoresis System ◽  
Selection Of

Abstract A selection of 58 specimens with a monoclonal component identified by immunoelectrophoresis and/or immunofixation was analyzed with the immunosubtraction procedure on the Paragon 2000 capillary electrophoresis system. The capillary system detected 93% of the paraproteins and, using immunosubtraction, correctly identified 91% of the paraproteins. Paraproteins that were detected by immunofixation and/or immunoelectrophoresis but not by capillary electrophoresis were also missed by agarose electrophoresis and cellulose acetate electrophoresis. Cellulose acetate electrophoresis was the least sensitive method for detection of paraproteins. Only 74% of the monoclonal components were detected by this technique, whereas 86% were revealed by agarose electrophoresis. In addition to monoclonal paraproteins, we also studied biclonal paraproteins and oligoclonal banding. Capillary electrophoresis and immunosubtraction correctly detected and identified three specimens containing biclonal paraproteins. In one specimen, capillary zone electrophoresis detected only one band, whereas agarose gel electrophoresis detected two bands. The sensitivity for detection and identification of oligoclonal banding by capillary electrophoresis was inferior to immunofixation.

A semipermanent coating for preventing protein adsorption at physiological pH in kinetic capillary electrophoresis

2012 ◽  
Vol 33 (16) ◽  
pp. 2584-2590 ◽  
Author(s):  
Stephanie de Jong ◽  
Nicolas Epelbaum ◽  
Ruchi Liyanage ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Protein Adsorption ◽  
Kinetic Capillary Electrophoresis

Kinetic Capillary Electrophoresis (KCE):  A Conceptual Platform for Kinetic Homogeneous Affinity Methods

2005 ◽  
Vol 127 (48) ◽  
pp. 17104-17110 ◽  
Author(s):  
Alexander Petrov ◽  
Victor Okhonin ◽  
Maxim Berezovski ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Kinetic Capillary Electrophoresis

High-efficiency selection of aptamers for bovine lactoferrin by capillary electrophoresis and its aptasensor application in milk powder

Talanta ◽  
2019 ◽  
Vol 205 ◽  
pp. 120088 ◽  
Author(s):  
Chao Zhu ◽  
Linsen Li ◽  
Ge Yang ◽  
Muhammad Irfan ◽  
Zijian Wang ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
High Efficiency ◽  
Milk Powder ◽  
Bovine Lactoferrin ◽  
Selection Of
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