Plug−Plug Kinetic Capillary Electrophoresis:  Method for Direct Determination of Rate Constants of Complex Formation and Dissociation

2006 ◽  
Vol 78 (14) ◽  
pp. 4803-4810 ◽  
Author(s):  
Victor Okhonin ◽  
Alexander P. Petrov ◽  
Maxim Berezovski ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Complex Formation ◽  
Rate Constants ◽  
Direct Determination ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method ◽  
Kinetic Capillary Electrophoresis

Establishing Tablet Dissolution Curve Through Determination of Underivatized Alendronate Sodium by Capillary Electrophoresis-UV Detector

2020 ◽  
Vol 16 (5) ◽  
pp. 615-622
Author(s):  
Tian Luo ◽  
Shujuan He ◽  
Yueting Deng ◽  
Ximin Zhang ◽  
Yuming Dong
Keyword(s):  
High Performance Liquid Chromatography ◽  
Capillary Electrophoresis ◽  
Liquid Chromatography ◽  
High Performance ◽  
Direct Determination ◽  
Dissolution Profile ◽  
Alendronate Sodium ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method

Background: Alendronate sodium is a common clinical osteoporosis drug for postmenopausal women; its determination is very important. However, there is no absorption of chromophores or fluorophores in the molecule, therefore, their direct determination is a challenge. Thus, establishing a common and direct method is very inspiring. Methods: According to the direct determination of alendronate sodium through the formation of a complex between alendronate sodium and divalent copper ion by capillary electrophoresis with ultraviolet detection, the dissolution profile of alendronate sodium tablet was established. The dissolution curves obtained from high-performance liquid chromatography method involving derivatization with 9- fluorenyl methylchloroformate and capillary electrophoresis with ultraviolet detector were found to be highly similar. Underivatized alendronate sodium can be determined by the capillary electrophoresis method. Results: Optimum conditions were as follows: background electrolyte including 25 mM CuSO4 at pH 4.59, 5 s injection time, 18 kV applied voltage, and 240 nm detected wavelength. Method validation indicated good linearity (r2>0.9993), precision of migration time with a relative standard deviation <1.5 % for intra-day and <3.6 % for inter-day, precision of peak areas <2.3 % for intra-day and <5.0 % for inter-day, limits of detection (0.01 μg/mL), limit of quantification (0.04 μg/mL) and recovery (90.6 %- 109.0 %). Conclusion: The proposed capillary electrophoresis method has been proved to be simpler, faster and more convenient to test dissolution profile of alendronate sodium tablet than that of high performance liquid chromatography.

scholarly journals Development of a fast capillary electrophoresis method for determination of carbohydrates in honey samples

Talanta ◽  
2012 ◽  
Vol 93 ◽  
pp. 62-66 ◽  
Author(s):  
Viviane Maria Rizelio ◽  
Laura Tenfen ◽  
Roberta da Silveira ◽  
Luciano Valdemiro Gonzaga ◽  
Ana Carolina Oliveira Costa ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method

A novel nonaqueous capillary electrophoresis method for effective separation and simultaneous determination of aurantio-obtusin, emodin and rhein in semen cassiae and cassia seed tea

2014 ◽  
Vol 6 (14) ◽  
pp. 5133-5139 ◽  
Author(s):  
Ning Wang ◽  
Yuanyuan Wu ◽  
Xingqiang Wu ◽  
Shuxuan Liang ◽  
Hanwen Sun
Keyword(s):  
Capillary Electrophoresis ◽  
Simultaneous Determination ◽  
Uv Detection ◽  
Nonaqueous Capillary Electrophoresis ◽  
Effective Separation ◽  
Capillary Electrophoresis Method ◽  
Electrophoresis Method ◽  
Novel Method

A novel method using nonaqueous capillary electrophoresis-UV detection was used for the effective separation and rapid simultaneous determination of anthraquinones, aurantio-obtusin, emodin and rhein, in semen cassiae and cassia seed tea.

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