Identification of Base Pairs in Single-Nucleotide Polymorphisms by MutS Protein-Mediated Capillary Electrophoresis

2006 ◽  
Vol 78 (6) ◽  
pp. 2035-2038 ◽  
Author(s):  
Andrei P. Drabovich ◽  
Sergey N. Krylov
Keyword(s):  
Capillary Electrophoresis ◽  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Base Pairs ◽  
Single Nucleotide

Approach to analysis of single nucleotide polymorphisms by automated constant denaturant capillary electrophoresis

2003 ◽  
Vol 526 (1-2) ◽  
pp. 75-83 ◽  
Author(s):  
Jens Bjørheim ◽  
Torveig Weum Abrahamsen ◽  
Annette Torgunrud Kristensen ◽  
Gustav Gaudernack ◽  
Per O Ekstrøm
Keyword(s):  
Capillary Electrophoresis ◽  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Sequence polymorphism from EST data in sugarcane: a fine analysis of 6-phosphogluconate dehydrogenase genes

2001 ◽  
Vol 24 (1-4) ◽  
pp. 161-167 ◽  
Author(s):  
L. Grivet ◽  
J.C. Glaszmann ◽  
P. Arruda
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Expressed Sequence Tag ◽  
Sequence Polymorphism ◽  
Nucleotide Polymorphisms ◽  
Base Pairs ◽  
Est Database ◽  
Single Nucleotide ◽  
Clear Cut ◽  
Phosphogluconate Dehydrogenase ◽  
Expressed Sequence

This paper presents preliminary results demonstrating the use of the sugarcane expressed sequence tag (EST) database (SUCEST) to detect single nucleotide polymorphisms (SNPs) inside 6-phosphogluconate dehydrogenase genes (Pgds). Sixty-four Pgd-related EST sequences were identified and partitioned into two clear-cut sets of 14 and 50 ESTs, probably corresponding to two genes, A and B, respectively. Alignment of A sequences allowed the detection of a single SNP while alignment of B sequences permitted the detection of 39 reliable SNPs, 27 of which in the coding sequence of the gene. Thirty-eight SNPs were binucleotidic and a single one was trinucleotidic. Nine insertions/deletions from one to 72 base pairs long were also detected in the noncoding 3’ and 5’ sequences. The soundness and the consequences of those preliminary observations on sequence polymorphism in sugarcane are discussed.

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