scholarly journals Optimization of Dielectrophoretic DNA Stretching in Microfabricated Devices

2006 ◽  
Vol 78 (9) ◽  
pp. 2939-2947 ◽  
Author(s):  
Kyung Eun Sung ◽  
Mark A. Burns
Keyword(s):  
Dna Stretching ◽  
Microfabricated Devices

Polymer dynamics and fluid flow in microfabricated devices

1998 ◽  
Author(s):  
Olgica B. Bakajin ◽  
James P. Brody ◽  
Jeff Chou ◽  
Shirley S. Chan ◽  
Thomas A. J. Duke ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Polymer Dynamics ◽  
Microfabricated Devices

scholarly journals Determining Trap Compliances, Microsphere Size Variations, and Response Linearities in Single DNA Molecule Elasticity Measurements with Optical Tweezers

2021 ◽  
Vol 8 ◽  
Author(s):  
Youbin Mo ◽  
Mounir Fizari ◽  
Kristina Koharchik ◽  
Douglas E. Smith
Keyword(s):  
Optical Tweezers ◽  
Dna Elasticity ◽  
Dna Stretching ◽  
Dna Molecule ◽  
Scale Factors ◽  
Length Measurements ◽  
Microsphere Size ◽  
Force Range ◽  
Single Dna Molecule ◽  
Displacement Measurements

We previously introduced the use of DNA molecules for calibration of biophysical force and displacement measurements with optical tweezers. Force and length scale factors can be determined from measurements of DNA stretching. Trap compliance can be determined by fitting the data to a nonlinear DNA elasticity model, however, noise/drift/offsets in the measurement can affect the reliability of this determination. Here we demonstrate a more robust method that uses a linear approximation for DNA elasticity applied to high force range (25–45 pN) data. We show that this method can be used to assess how small variations in microsphere sizes affect DNA length measurements and demonstrate methods for correcting for these errors. We further show that these measurements can be used to check assumed linearities of system responses. Finally, we demonstrate methods combining microsphere imaging and DNA stretching to check the compliance and positioning of individual traps.

Protein assembly onto patterned microfabricated devices through enzymatic activation of fusion pro-tag

2007 ◽  
Vol 99 (3) ◽  
pp. 499-507 ◽  
Author(s):  
Angela T. Lewandowski ◽  
Hyunmin Yi ◽  
Xiaolong Luo ◽  
Gregory F. Payne ◽  
Reza Ghodssi ◽  
...  
Keyword(s):  
Protein Assembly ◽  
Enzymatic Activation ◽  
Microfabricated Devices

scholarly journals Theory of high-force DNA stretching and overstretching

2003 ◽  
Vol 67 (5) ◽  
Author(s):  
C. Storm ◽  
P. C. Nelson
Keyword(s):  
Dna Stretching

scholarly journals DNA Interactions Kinetics of HIV-1 Nucleocapsid Proteins by Single Molecule DNA Stretching

2012 ◽  
Vol 102 (3) ◽  
pp. 484a
Author(s):  
Jialin Li ◽  
Robert Gorelick ◽  
Ioulia Rouzina ◽  
Karin Musier-Forsyth ◽  
Mark Williams
Keyword(s):  
Single Molecule ◽  
Dna Interactions ◽  
Dna Stretching ◽  
Nucleocapsid Proteins ◽  
Kinetics Of ◽  
Hiv 1
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