Surface Binding Affinity Measurements from Order Transitions of Lipid Membrane-Coated Colloidal Particles

2006 ◽  
Vol 78 (1) ◽  
pp. 174-180 ◽  
Author(s):  
Esther M. Winter ◽  
Jay T. Groves
Keyword(s):  
Binding Affinity ◽  
Colloidal Particles ◽  
Lipid Membrane ◽  
Surface Binding

scholarly journals Direct visualization of superselective colloid-surface binding mediated by multivalent interactions

2021 ◽  
Vol 118 (36) ◽  
pp. e2106036118
Author(s):  
Christine Linne ◽  
Daniele Visco ◽  
Stefano Angioletti-Uberti ◽  
Liedewij Laan ◽  
Daniela J. Kraft
Keyword(s):  
Colloidal Particles ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Model System ◽  
Receptor Density ◽  
Mobile Dna ◽  
Surface Binding ◽  
Receptor Level ◽  
Foerster Resonance Energy Transfer ◽  
Multivalent Interactions

Reliably distinguishing between cells based on minute differences in receptor density is crucial for cell–cell or virus–cell recognition, the initiation of signal transduction, and selective targeting in directed drug delivery. Such sharp differentiation between different surfaces based on their receptor density can only be achieved by multivalent interactions. Several theoretical and experimental works have contributed to our understanding of this “superselectivity.” However, a versatile, controlled experimental model system that allows quantitative measurements on the ligand–receptor level is still missing. Here, we present a multivalent model system based on colloidal particles equipped with surface-mobile DNA linkers that can superselectively target a surface functionalized with the complementary mobile DNA-linkers. Using a combined approach of light microscopy and Foerster resonance energy transfer (FRET), we can directly observe the binding and recruitment of the ligand–receptor pairs in the contact area. We find a nonlinear transition in colloid-surface binding probability with increasing ligand or receptor concentration. In addition, we observe an increased sensitivity with weaker ligand–receptor interactions, and we confirm that the timescale of binding reversibility of individual linkers has a strong influence on superselectivity. These unprecedented insights on the ligand–receptor level provide dynamic information into the multivalent interaction between two fluidic membranes mediated by both mobile receptors and ligands and will enable future work on the role of spatial–temporal ligand–receptor dynamics on colloid-surface binding.

Cell surface binding affinity of Simian virus 40 T-antigen

Virology ◽  
1982 ◽  
Vol 117 (1) ◽  
pp. 173-185 ◽  
Author(s):  
Jutta Lange-Mutschler ◽  
Roland Henning
Keyword(s):  
Cell Surface ◽  
Binding Affinity ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Surface Binding ◽  
Cell Surface Binding

scholarly journals Lipid membrane-mediated attraction between curvature inducing objects

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Casper van der Wel ◽  
Afshin Vahid ◽  
Anđela Šarić ◽  
Timon Idema ◽  
Doris Heinrich ◽  
...  
Keyword(s):  
Colloidal Particles ◽  
Lipid Membrane ◽  
Numerical Study ◽  
Particle Diameter ◽  
Interaction Force ◽  
Membrane Curvature ◽  
Coarse Grained ◽  
Cellular Transport ◽  
Curvature Energy ◽  
Membrane Deformations

Abstract The interplay of membrane proteins is vital for many biological processes, such as cellular transport, cell division, and signal transduction between nerve cells. Theoretical considerations have led to the idea that the membrane itself mediates protein self-organization in these processes through minimization of membrane curvature energy. Here, we present a combined experimental and numerical study in which we quantify these interactions directly for the first time. In our experimental model system we control the deformation of a lipid membrane by adhering colloidal particles. Using confocal microscopy, we establish that these membrane deformations cause an attractive interaction force leading to reversible binding. The attraction extends over 2.5 times the particle diameter and has a strength of three times the thermal energy (−3.3 kBT). Coarse-grained Monte-Carlo simulations of the system are in excellent agreement with the experimental results and prove that the measured interaction is independent of length scale. Our combined experimental and numerical results reveal membrane curvature as a common physical origin for interactions between any membrane-deforming objects, from nanometre-sized proteins to micrometre-sized particles.

scholarly journals Surfactant-free Colloidal Particles with Specific Binding Affinity

Langmuir ◽  
2017 ◽  
Vol 33 (38) ◽  
pp. 9803-9810 ◽  
Author(s):  
Casper van der Wel ◽  
Nelli Bossert ◽  
Quinten J. Mank ◽  
Marcel G. T. Winter ◽  
Doris Heinrich ◽  
...  
Keyword(s):  
Binding Affinity ◽  
Colloidal Particles ◽  
Specific Binding

ChemInform Abstract: Lipid Membrane Supported by Colloidal Particles

ChemInform ◽  
2008 ◽  
Vol 39 (6) ◽  
Author(s):  
Anne-Lise Troutier ◽  
Catherine Ladaviere
Keyword(s):  
Colloidal Particles ◽  
Lipid Membrane

An overview of lipid membrane supported by colloidal particles

2007 ◽  
Vol 133 (1) ◽  
pp. 1-21 ◽  
Author(s):  
Anne-Lise Troutier ◽  
Catherine Ladavière
Keyword(s):  
Colloidal Particles ◽  
Lipid Membrane

Ultrastructure of Lymphatic Capillaries in the Transport of Colloidal Particles

1967 ◽  
Vol 25 ◽  
pp. 186-187
Author(s):  
L. V. Leak ◽  
J. F. Burke
Keyword(s):  
Connective Tissue ◽  
Colloidal Particles ◽  
Ultrastructural Level ◽  
Vital Role ◽  
Time Intervals ◽  
Thorium Dioxide ◽  
Lymphatic Capillary ◽  
Tissue Area ◽  
Rapid Removal ◽  
Tissue Fluids

The vital role played by the lymphatic capillaries in the transfer of tissue fluids and particulate materials from the connective tissue area can be demonstrated by the rapid removal of injected vital dyes into the tissue areas. In order to ascertain the mechanisms involved in the transfer of substances from the connective tissue area at the ultrastructural level, we have injected colloidal particles of varying sizes which range from 80 A up to 900-mμ. These colloidal particles (colloidal ferritin 80-100A, thorium dioxide 100-200 A, biological carbon 200-300 and latex spheres 900-mμ) are injected directly into the interstitial spaces of the connective tissue with glass micro-needles mounted in a modified Chambers micromanipulator. The progress of the particles from the interstitial space into the lymphatic capillary lumen is followed by observing tissues from animals (skin of the guinea pig ear) that were injected at various time intervals ranging from 5 minutes up to 6 months.

Deposited emulsion particles in the pores of aluminum oxide film

1978 ◽  
Vol 36 (1) ◽  
pp. 450-451
Author(s):  
Michio Ashida ◽  
Yasukiyo Ueda
Keyword(s):  
Oxide Film ◽  
Acid Solution ◽  
Barrier Layer ◽  
Colloidal Particles ◽  
Oxalic Acid Solution ◽  
Anodic Oxide ◽  
Anodic Oxide Film ◽  
Carbon Replica ◽  
Sectioning Method ◽  
Thin Sectioning

An anodic oxide film is formed on aluminum in an acidic elecrolyte during anodizing. The structure of the oxide film was observed directly by carbon replica method(l) and ultra-thin sectioning method(2). The oxide film consists of barrier layer and porous layer constructed with fine hexagonal cellular structure. The diameter of micro pores and the thickness of barrier layer depend on the applying voltage and electrolyte. Because the dimension of the pore corresponds to that of colloidal particles, many metals deposit in the pores. When the oxide film is treated as anode in emulsion of polyelectrolyte, the emulsion particles migrate onto the film and deposit on it. We investigated the behavior of the emulsion particles during electrodeposition.Aluminum foils (99.3%) were anodized in either 0.25M oxalic acid solution at 30°C or 3M sulfuric acid solution at 20°C. After washing with distilled water, the oxide films used as anode were coated with emulsion particles by applying voltage of 200V and then they were cured at 190°C for 30 minutes.

COUPLING OF COLLOIDAL PARTICLES AND RECOILLESS FRACTION

1976 ◽  
Vol 37 (C6) ◽  
pp. C6-273-C6-276
Author(s):  
H. J. ÜBELHACK ◽  
F. H. WITTMANN
Keyword(s):  
Colloidal Particles ◽  
Recoilless Fraction
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