Efficient and Specific Trypsin Digestion of Microgram to Nanogram Quantities of Proteins in Organic−Aqueous Solvent Systems

2006 ◽  
Vol 78 (1) ◽  
pp. 125-134 ◽  
Author(s):  
Michael Brad Strader ◽  
David L. Tabb ◽  
W. Judson Hervey ◽  
Chongle Pan ◽  
Gregory B. Hurst
Keyword(s):  
Trypsin Digestion ◽  
Aqueous Solvent ◽  
Solvent Systems

Oxidation of lignans and lignin model compounds by laccase in aqueous solvent systems

2011 ◽  
Vol 72 (3-4) ◽  
pp. 122-129 ◽  
Author(s):  
Maija-Liisa Mattinen ◽  
Pekka Maijala ◽  
Paula Nousiainen ◽  
Annika Smeds ◽  
Jussi Kontro ◽  
...  
Keyword(s):  
Model Compounds ◽  
Lignin Model Compounds ◽  
Aqueous Solvent ◽  
Lignin Model ◽  
Solvent Systems

Lifetime enhancement of the first electronic triplet state of 3,5-dinitroanisole by hydrogen bonding to clusters in mixed aqueous solvent systems

1977 ◽  
Vol 22 (3) ◽  
pp. 475-483 ◽  
Author(s):  
C.A.G.O. Varma ◽  
F.L. Plantenga ◽  
C.A.M. van den Ende ◽  
P.H.M. van Zeyl ◽  
J.J. Tamminga ◽  
...  
Keyword(s):  
Hydrogen Bonding ◽  
Triplet State ◽  
Aqueous Solvent ◽  
Solvent Systems ◽  
Lifetime Enhancement

Isolation and Purification of Five Phloroglucinol Derivatives From Agrimonia Pilosa Ledeb by Counter-Current Chromatography Using Non-Aqueous Solvent Systems

2020 ◽  
Author(s):  
Lulu Fu ◽  
Chenghua Lu ◽  
Shengqiang Tong ◽  
Min Xu ◽  
Lan Tang ◽  
...  
Keyword(s):  
Closed Loop ◽  
Solvent System ◽  
The Other ◽  
Second Step ◽  
Two Phase ◽  
Isolation And Purification ◽  
Aqueous Solvent ◽  
Phloroglucinol Derivatives ◽  
Solvent Systems ◽  
Counter Current

Abstract Five non-polar phloroglucinol derivatives, viz. pseudo-aspidin, α-kosin and agripinol A-C were isolated and purified from Agrimonia pilosa Ledeb by semi-preparative counter-current chromatography. The separation was performed by a two-step elution with non-aqueous solvent systems. In the first step, an elution mode of a two-phase solvent system consisting of n-hexane-acetonitrile-dichloromethane-methanol (6:6:0.5:0.5, v/v/v/v) was used. We obtained sample Ι containing three components (47.0 mg) and sample ΙΙ containing two components (24.8 mg) from crude extract (371.0 mg). In the second step, sample Ι was successfully separated by closed-loop recycling mode with a solvent system consisting of n-hexane-acetonitrile-dichloromethane (10:7:3, v/v/v), yielding 17.8 mg of pseudo-aspidin, 18.5 mg of α-kosin and 6.4 mg of agripinol A. The other two compounds—8.7 mg of agripinol B and 13.6 mg of agripinol C—were obtained from sample ΙΙ in the same manner. All the isolated compounds had a high purity exceeding 95%.

Effects of photochemical reactions of pyrene in alcohol and aqueous solvent systems on spectroscopic analyses

1996 ◽  
Vol 319 (3) ◽  
pp. 379-386 ◽  
Author(s):  
Ya-Ping Sun ◽  
Bin Ma ◽  
Glenn E. Lawson ◽  
Christopher E. Bunker ◽  
Harry W. Rollins
Keyword(s):  
Photochemical Reactions ◽  
Spectroscopic Analyses ◽  
Aqueous Solvent ◽  
Solvent Systems
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