Determination of Ribonuclease H Surface Enzyme Kinetics by Surface Plasmon Resonance Imaging and Surface Plasmon Fluorescence Spectroscopy

2005 ◽  
Vol 77 (20) ◽  
pp. 6528-6534 ◽  
Author(s):  
Shiping Fang ◽  
Hye Jin Lee ◽  
Alastair W. Wark ◽  
Hyun Min Kim ◽  
Robert M. Corn
Keyword(s):  
Surface Plasmon Resonance ◽  
Fluorescence Spectroscopy ◽  
Enzyme Kinetics ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Ribonuclease H ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

An immunosensor for the determination of carcinoembryonic antigen by Surface Plasmon Resonance imaging

2020 ◽  
Vol 609 ◽  
pp. 113964
Author(s):  
Beata Szymanska ◽  
Zenon Lukaszewski ◽  
Kinga Hermanowicz-Szamatowicz ◽  
Ewa Gorodkiewicz
Keyword(s):  
Surface Plasmon Resonance ◽  
Carcinoembryonic Antigen ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

SPRI biosensors for quantitative determination of matrix metalloproteinase-2

2017 ◽  
Vol 9 (16) ◽  
pp. 2407-2414 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Iosif Sveklo ◽  
Ewa Matuszczak ◽  
Adam Hermanowicz ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Matrix Metalloproteinase ◽  
Quantitative Determination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Matrix Metalloproteinase 2 ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

The aim of this study was to develop a new, label-free, highly selective Surface Plasmon Resonance Imaging biosensor for the quantitative determination of matrix metalloproteinase-2.

scholarly journals A New Analytical Method for Determination of Cathepsin L Based on the Surface Plasmon Resonance Imaging Biosensor

2019 ◽  
Vol 20 (9) ◽  
pp. 2166 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Anna Sankiewicz ◽  
Adam Hermanowicz ◽  
Krzysztof Sobolewski ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Blood Plasma ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cathepsin L ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Standard Curve

The purpose of this study was to develop a new method for a determination of the cathepsin L—biosensor based on the Surface Plasmon Resonance Imaging technique. The cathepsin L is an endopeptidase, which degrades proteins and plays an important role in various processes occurring in the human body. The detection technique, Surface Plasmon Resonance Imaging, is an optical, label-free technique, which can be used for quantitative determination of the different proteins. In order to bind the enzyme, the cathepsin L inhibitor—RKLLW-NH2 was used. The validation process showed that parameters: precision, accuracy, and selectivity of the method were acceptable. The analytically useful range of the standard curve was 0.50 ng/mL—15.00 ng/mL. The detection and quantification limit of method was 1.67 pg/mL and 5.07 pg/mL, respectively. The usefulness of the developed method was confirmed by the determination of the cathepsin L concentration in the blood plasma of some healthy persons and in the blood plasma of patients. The obtained results were compared with the results obtained by the ELISA. It was found that the correlation between these two methods was very strong, what suggest that the developed method can be used as the competitive method to the ELISA.

Lectin recognition of a new SOD mimic bioconjugate studied with surface plasmon resonance imaging

2006 ◽  
Vol 4 (4) ◽  
pp. 610 ◽  
Author(s):  
Roberta D'Agata ◽  
Giulia Grasso ◽  
Giuseppe Iacono ◽  
Giuseppe Spoto ◽  
Graziella Vecchio
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Lectin Recognition
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