High-Resolution Length Sorting and Purification of DNA-Wrapped Carbon Nanotubes by Size-Exclusion Chromatography

2005 ◽  
Vol 77 (19) ◽  
pp. 6225-6228 ◽  
Author(s):  
Xueying Huang ◽  
Robert S. Mclean ◽  
Ming Zheng
Keyword(s):  
Carbon Nanotubes ◽  
High Resolution ◽  
Size Exclusion Chromatography ◽  
Size Exclusion ◽  
Exclusion Chromatography

UNRAVELING THE MYSTERY OF NATURAL RUBBER BIOSYNTHESIS. PART II: COMPOSITION AND GROWTH OF IN VITRO NATURAL RUBBER USING HIGH-RESOLUTION SIZE EXCLUSION CHROMATOGRAPHY

2014 ◽  
Vol 87 (3) ◽  
pp. 451-458 ◽  
Author(s):  
Cheng Ching K. Chiang ◽  
Balaka Barkakaty ◽  
Judit E. Puskas ◽  
Wenshuang Xie ◽  
Katrina Cornish ◽  
...  
Keyword(s):  
High Resolution ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Rubber Tree ◽  
Size Exclusion ◽  
Common Source ◽  
Rubber Biosynthesis ◽  
Flight Mass Spectrometry ◽  
Exclusion Chromatography

ABSTRACT The superior properties of natural rubber (cis-1,4-polyisoprene [NR]) are a function of its structure and composition, properties that still remain a mystery and that are irreplaceable by any synthetic rubber. NR from guayule (Parthenium argentatum) has been gaining special interest for its hypoallergenic properties while maintaining superior mechanical properties that are commonly associated with the Brazilian rubber tree (Hevea brasiliensis), the most common source of NR. Techniques exist to isolate washed rubber particles (WRPs) that contain enzymatically active rubber transferase, to study NR biosynthesis, and previous work on the in vitro NR growth in Hevea has demonstrated the presence of around 50 wt% of a low molecular weight ([MW], Mn <10 000 g/mol) fraction. Structural and compositional analyses of this low MW fraction in Hevea are challenging due to the high protein content. We discuss the analysis and composition of guayule latex and WRPs using high-resolution Size Exclusion Chromatography. We also discuss the composition of the soluble fraction of inactive guayule latex using matrix-assisted laser desorption ionization/time of flight mass spectrometry.

UNRAVELING THE MYSTERY OF NATURAL RUBBER BIOSYNTHESIS. PART I: INVESTIGATION OF THE COMPOSITION AND GROWTH OF IN VITRO NATURAL RUBBER USING HIGH RESOLUTION SIZE EXCLUSION CHROMATOGRAPHY

2011 ◽  
Vol 84 (2) ◽  
pp. 166-177 ◽  
Author(s):  
Chengching K. Chiang ◽  
Wenshuang Xie ◽  
Colleen McMahan ◽  
Judit E. Puskas
Keyword(s):  
High Resolution ◽  
Natural Rubber ◽  
Size Exclusion Chromatography ◽  
Weight Fraction ◽  
Size Exclusion ◽  
Gravimetric Analysis ◽  
In Vitro Synthesis ◽  
Rubber Biosynthesis ◽  
Exclusion Chromatography

Abstract Monitoring the growth of in vitro natural rubber was accomplished by high resolution size exclusion chromatography, SEC. Washed rubber particles isolated from H. brasiliensis latex, containing the rubber transferase enzyme, were used to catalyze the polymerization of synthetic isopentenyl pyrophosphate monomer in the presence of farnesyl pyrophosphate initiator. The high-resolution SEC was able to detect the formation of new rubber. Changes in the low molecular weight fraction were also detected. Gravimetric analysis revealed ∼30% mass gain after the in vitro synthesis. The overall gel content was found to be reduced, which further supported the formation of new rubber. This is the first report that utilizes high-resolution SEC to monitor the in vitro NR growth without the use of radiolabeling.

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