Molecular Shape Selectivity through Multiple Carbonyl−π Interactions with Noncrystalline Solid Phase for RP-HPLC

2005 ◽  
Vol 77 (20) ◽  
pp. 6671-6681 ◽  
Author(s):  
M. Mizanur Rahman ◽  
Makoto Takafuji ◽  
Hamid R. Ansarian ◽  
Hirotaka Ihara
Keyword(s):  
Solid Phase ◽  
Shape Selectivity ◽  
Molecular Shape ◽  
Π Interactions ◽  
Rp Hplc

scholarly journals Conformational Effect of Silica-supported Poly(octadecyl acrylate) on Molecular-Shape Selectivity of Polycyclic Aromatic Hydrocarbons in RP-HPLC

2004 ◽  
Vol 20 (12) ◽  
pp. 1681-1685 ◽  
Author(s):  
Makoto TAKAFUJI ◽  
Megumi FUKUI ◽  
Hamid R. ANSARIAN ◽  
Mahnaz DERAKHSHAN ◽  
Atsuomi SHUNDO ◽  
...  
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Shape Selectivity ◽  
Molecular Shape ◽  
Rp Hplc ◽  
Conformational Effect ◽  
Polycyclic Aromatic

scholarly journals Sensitive Mercury Speciation by Reversed-Phase Column High-Performance Liquid Chromatography with UV-Visible Detection After Solid-Phase Extraction Using 6-Mercaptopurine and Dithizone

2008 ◽  
Vol 91 (6) ◽  
pp. 1453-1458 ◽  
Author(s):  
Hamid Hashemi-Moghaddam ◽  
Mohamad Saber-Tehrani
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Reversed Phase ◽  
Phase Extraction ◽  
Organic Mercury ◽  
Tuna Fish ◽  
Rp Hplc ◽  
Uv Visible ◽  
Phase Column ◽  
Reversed Phase Column

Abstract A highly selective and sensitive method was developed for preconcentration of inorganic and organic mercury compounds followed by reversed-phase column high-performance liquid chromatography (RP-HPLC) with UV-visible detection. The method was based on the reaction of mercury with 6-mercaptopurine and solid-phase extraction (SPE) of the complex on an octadecylsilane (C18) cartridge. The complex was then treated with ammoniacal dithizone solution, and the complexes of inorganic and organic mercury with dithizone were eluted by methanol. The speciation analysis of methylmercury (MeHg), phenylmercury (PhHg), and inorganic Hg (II) was carried out by RP-HPLC. Some experimental variables that influence the SPE and derivatization, such as pH, chelating and derivatizing agent concentration, and surfactant addition, were investigated. The calibration graphs of MeHg, PhHg, and Hg (II) were linear [correlation coefficient (r) > 0.999] from the detection limits (0.12, 0.16, and 0.14 ng) to 8.5, 6.0, and 6.7 ng Hg, respectively. By applying the SPE procedure, a 100-fold concentration of the sample was obtained. The procedure was applied to sea water and tuna fish samples. The method's accuracy was investigated by using tuna fish certified reference material BCR 464 and by spiking the samples with different amounts of MeHg, PhHg, and Hg (II). The average recoveries of MeHg, PhHg, and Hg (II) from spiked samples (0.12.0 g/L Hg) were 96 4, 98 3, and 104 4, respectively.

scholarly journals Development and Validation of RP-HPLC Method for Azilsartan Medoxomil Potassium Quantitation in Human Plasma by Solid Phase Extraction Procedure

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Paras P. Vekariya ◽  
Hitendra S. Joshi
Keyword(s):  
Solid Phase Extraction ◽  
Human Plasma ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Hplc Method ◽  
Array Detector ◽  
Phase Extraction ◽  
Rp Hplc ◽  
Limit Of Quantitation ◽  
Azilsartan Medoxomil

Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using solid phase extraction (SPE) technique for the determination of Azilsartan Medoxomil Potassium (AMP) in human plasma; detection was carried out by photo diode array detector. Chromatographic separation of the analyte AMP was achieved within 7.5 min by Waters symmetry C18 (4.6 × 250 mm, 5 µm) column, mobile phase was 25 mM ammonium acetate buffer (pH 5.5): acetonitrile 55 : 45 v/v, flow rate was 1.0 mL/min, and the detection was carried out at 254 nm. Calibration curve was linear (r2 > 0.9985) in the range of 1.0–9.0 µg/mL, limit of detection (LOD) and limit of quantitation (LOQ) were 0.150 µg/mL and 0.400 µg/mL, respectively, and intra- and interday deviations were between 1.53–8.41% and 1.78–4.59%, respectively. The overall mean recovery of AMP was 92.35%. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC method was successfully validated and may be applied to conduct bioavailability and bioequivalence studies of AMP.

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