Identification of Bacteria by Polymerase Chain Reaction Followed by Liquid Chromatography−Mass Spectrometry

2005 ◽  
Vol 77 (14) ◽  
pp. 4563-4570 ◽  
Author(s):  
Bettina M. Mayr ◽  
Uwe Kobold ◽  
Martin Moczko ◽  
Agnes Nyeki ◽  
Thomas Koch ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Polymerase Chain Reaction ◽  
Liquid Chromatography ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chain Reaction ◽  
Chromatography Mass Spectrometry ◽  
Identification Of Bacteria ◽  
Polymerase Chain

scholarly journals A Rapid and Reliable Liquid Chromatography/Mass Spectrometry Method for SARS-CoV-2 Diagnostics from Gargle Solutions and Saliva

2021 ◽  
Author(s):  
Marc Kipping ◽  
Dirk Taenzler ◽  
Andrea Sinz
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Direct Detection ◽  
Quantitative Polymerase Chain Reaction ◽  
Multiple Reaction Monitoring ◽  
Diagnostic Tools ◽  
Liquid Chromatography Mass Spectrometry ◽  
Clinical Environment ◽  
Chromatography Mass Spectrometry ◽  
Polymerase Chain

We describe a rapid liquid chromatography/mass spectrometry (LC/MS) method for the direct detection and quantitation of SARS-CoV-2 nucleoprotein in gargle solutions and saliva. The method is based on a multiple-reaction monitoring (MRM) mass spectrometry approach with a total cycle time of 5 minutes per analysis and allows the detection and accurate quantitation of SARS-CoV-2 nucleoprotein as low as 500 amol/ul. We improved the sample preparation protocol of our recent piloting SARS-CoV-2 LC/MS study regarding sensitivity, reproducibility, and compatibility with a complementary reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) analysis of the same sample. The aim of this work is to promote diagnostic tools that allow identifying and monitoring SARS-CoV-2 infections by LC/MS methods in a routine clinical environment.

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