Electrokinetically Based Approach for Single-Nucleotide Polymorphism Discrimination Using a Microfluidic Device

2005 ◽  
Vol 77 (13) ◽  
pp. 4000-4007 ◽  
Author(s):  
David Erickson ◽  
Xuezhu Liu ◽  
Roberto Venditti ◽  
Dongqing Li ◽  
Ulrich J. Krull
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Microfluidic Device ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

scholarly journals A microfluidic device for multiplex single-nucleotide polymorphism genotyping

RSC Advances ◽  
2014 ◽  
Vol 4 (9) ◽  
pp. 4269-4277 ◽  
Author(s):  
Jing Zhu ◽  
Chunmei Qiu ◽  
Mirkó Palla ◽  
ThaiHuu Nguyen ◽  
James J. Russo ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Microfluidic Device ◽  
Single Nucleotide Polymorphism Genotyping ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide

UTILIZATION OF DNA MARKERS FOR EVALUATING AMYLOSE CONTENT OF GRAIN IN RICE

2013 ◽  
Vol 83 (5) ◽  
Author(s):  
Dương Thanh Thủy ◽  
Taiichiro Ookawa
Keyword(s):  
Single Nucleotide Polymorphism ◽  
Amylose Content ◽  
Waxy Gene ◽  
Nucleotide Polymorphism ◽  
Single Nucleotide ◽  
Branching Enzymes ◽  
Wx Gene ◽  
High Amylose ◽  
Suitable Marker ◽  
Granule Bound Starch Synthase

The sensory and functional properties of rice are predominantly associated with its amylose content. Granule-bound starch synthase (GBSS) encoded by the Waxy (Wx) gene determines the synthesis of amylose, while starch branching enzymes encoded by Sbe genes are involved in the formation of amylopectin. Some studies have demonstrated that Wx gene is the major controller of amylose content but there are one or more modifying genes affecting the amylose content. Three markers,  microsatellite, Single – nucleotide – polymorphism (G/T SNP) in Wx gene and Single – nucleotide – polymorphism (T/C SNP) in Sbe1 gene, were tested for their association with amylose content using sixty-nine  rice accessions from twenty countries. Of the three markers, two markers in Wx gene are significantly associated with amylose content. The combination of two markers in Wx gene (haplotypes) explained 83.8% of the variation in amylose content and discriminated the three market classes of glutinous, low, intermediate and high amylose content of rice from each other. And T/C SNP in Sbe1 locus was not a suitable marker for amylose content. Keywords: marker, amylose content, Waxy gene.

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