Detection of Glutamate in Optically Trapped Single Nerve Terminals by Raman Spectroscopy

2004 ◽  
Vol 76 (9) ◽  
pp. 2506-2510 ◽  
Author(s):  
Katsuhiro Ajito ◽  
Chunxi Han ◽  
Keiichi Torimitsu
Keyword(s):  
Raman Spectroscopy ◽  
Nerve Terminals ◽  
Single Nerve ◽  
Optically Trapped

scholarly journals Correction: Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
Author(s):  
Aseefhali Bankapur ◽  
R. Sagar Krishnamurthy ◽  
Elsa Zachariah ◽  
Chidangil Santhosh ◽  
Basavaraj Chougule ◽  
...  
Keyword(s):  
Stem Cells ◽  
Raman Spectroscopy ◽  
Silver Nanoparticle ◽  
Micro Raman Spectroscopy ◽  
Induced Stress ◽  
Optically Trapped

An intracellular study of chemosensory fibers and endings

1980 ◽  
Vol 44 (6) ◽  
pp. 1077-1088 ◽  
Author(s):  
Y. Hayashida ◽  
H. Koyano ◽  
C. Eyzaguirre
Keyword(s):  
Carotid Body ◽  
Action Potentials ◽  
Physiological Saline ◽  
Mean Amplitude ◽  
Nerve Fibers ◽  
Chemical Stimulation ◽  
Nerve Terminals ◽  
Generator Potential ◽  
Single Nerve ◽  
Frequency Changes

1. The carotid body and its nerve, removed from anesthetized cats, were placed in physiological saline flowing under paraffin oil. The nerve, lifted into the oil, was used for either electrical stimulation or recording of the total afferent discharge. Intracellular recordings were obtained from individual nerve fibers and endings within the carotid body. The recording sites were identified by injecting Procion yellow through the intracellular electrodes; the tissues were then prepared for histology and observed with episcopic fluorescence or Nomarski optics. 2. Intracellularly recorded chemosensory fibers conducted at 1.1-30 m/s and usually displayed action potentials of regular amplitude. At times, however, some spikes become partially blocked while others maintained their original amplitude. "Natural" (hypoxia) or chemical (ACh or NaCN) stimulation induced different patterns of frequency changes of the large and small action potentials. This indicated nerve fiber branching at some distance from the recording site. 3. Intra- and extracellularly recorded spikes were blocked in 0 [Na+]0 by tetrodotoxin (TTX) or procaine. 4. During chemical stimulation, a slowly occurring depolarization (receptor or generator potential) was recorded intracellularly from the afferent fibers. It developed concomitantly with the increase in discharge. 5. Impalement of single nerve terminals (histologically identified) showed numerous "spontaneous" depolarizing potentials (SDPs) that had a mean amplitude of 5.6 mV, a mean duration of 46.1 ms, and nearly random distribution. They increased in frequency and summated during chemical stimulation. SDPs originated from either the site of recording or from neighboring areas. When the SDPs attained a certain amplitude, they seemed to give rise to action potentials. Also, relatively well developed or partially blocked spikes (apparently originating elsewhere) were recorded from single nerve terminals. 6. The receptor (generator) potential of chemosensory receptors appears to be an integrated response formed by multiple activity originating in different nerve endings.

Raman spectroscopy of optically trapped single aerosol particles

1991 ◽  
Vol 22 ◽  
pp. S427-S430 ◽  
Author(s):  
Günter Georg Hoffmann ◽  
Bernhard Oelichmann ◽  
Bernhard Schrader
Keyword(s):  
Raman Spectroscopy ◽  
Aerosol Particles ◽  
Optically Trapped

scholarly journals Normal saline-induced deoxygenation of red blood cells probed by optical tweezers combined with the micro-Raman technique

RSC Advances ◽  
2019 ◽  
Vol 9 (14) ◽  
pp. 7878-7884 ◽  
Author(s):  
Jijo Lukose ◽  
Mithun N ◽  
Ganesh Mohan ◽  
Shamee Shastry ◽  
Santhosh Chidangil
Keyword(s):  
Raman Spectroscopy ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Optical Tweezers ◽  
Red Blood Cell ◽  
Normal Saline ◽  
Blood Cells ◽  
Micro Raman Spectroscopy ◽  
Optically Trapped

Micro-Raman spectroscopy of optically trapped live red blood cell demonstrates normal saline induced deoxygenation.

scholarly journals Real-time Raman spectroscopy of optically trapped living cells and organelles

2004 ◽  
Vol 12 (25) ◽  
pp. 6208 ◽  
Author(s):  
Changan Xie ◽  
Charles Goodman ◽  
Mumtaz A. Dinno ◽  
Yong-Qing Li
Keyword(s):  
Raman Spectroscopy ◽  
Real Time ◽  
Living Cells ◽  
Optically Trapped

scholarly journals Raman Spectroscopy of Optically Trapped Single Biological Micro-Particles

Sensors ◽  
2015 ◽  
Vol 15 (8) ◽  
pp. 19021-19046 ◽  
Author(s):  
Brandon Redding ◽  
Mark Schwab ◽  
Yong-le Pan
Keyword(s):  
Raman Spectroscopy ◽  
Micro Particles ◽  
Optically Trapped
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