Theory and Simulation of Surface Plasmon-Coupled Directional Emission from Fluorophores at Planar Structures

2004 ◽  
Vol 76 (8) ◽  
pp. 2168-2173 ◽  
Author(s):  
Nils Calander
Author(s):  
Nikolay S. Panamarev ◽  
Dmitriy V. Apeksimov ◽  
Valeriy A. Donchenko ◽  
Alexey A. Zemlyanov ◽  
Ignatiy V. Samokhvalov ◽  
...  

2004 ◽  
Vol 9 (3) ◽  
pp. 208-215 ◽  
Author(s):  
Joanna Malicka ◽  
Ignacy Gryczynski ◽  
Zygmunt Gryczynski ◽  
Joseph R. Lakowicz

The authors describe a new approach to measuring DNA hybridization based on surface plasmon-coupled emission (SPCE). SPCE is the resonance coupling of excited fluorophores with electron motions in thin metal films, resulting in efficient transfer of energy through the film and radiation into the glass substrate. The authors evaluated the use of SPCE for detection of DNA hybridization. An unlabeled capture biotinylated oligonucleotide was attached near the surface of a thin (50 nm) silver film using streptavidin. The authors then measured the emission intensity of single-stranded Cy5-labeled DNA upon binding to a complementary oligomer attached to a silver film. Hybridization could be detected by an increase in SPCE, which appeared as light radiated into the substrate at a sharply defined angle near 73 degrees from the normal. The largest signals were observed when the excitation angle of incidence equaled the surface plasmon wavelength, but directional emission was also observed without excitation by the surface plasmon evanescent field. The increased intensity is due to proximity to the metal surface, so that hybridization can be detected without a change in the quantum yield of the fluorophore. These results indicate that SPCE can provide highly sensitive real-time measurement of DNA hybridization.


Nanophotonics ◽  
2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Edson P. Bellido ◽  
Isobel C. Bicket ◽  
Gianluigi A. Botton

Abstract In this work, we investigate the effects of bends on the surface plasmon resonances in nanowires (NWs) and isolated edges of planar structures using electron energy loss spectroscopy experiments and theoretical calculations. Previous work showed that the sharp bends in NWs do not affect their resonant modes. Here, we study previously overlooked effects and analyze systematically the evolution of resonant modes for several bending angles from 30° to 180°, showing that bending can have a significant effect on the plasmonic response of a nanostructure. In NWs, the modes can experience significant energy shifts that depend on the aspect ratio of the NW and can cause mode intersection and antinode bunching. We establish the relation between NW modes and edge modes and show that bending can even induce antinode splitting in edge modes. This work demonstrates that bends in plasmonic planar nanostructures can have a profound effect on their optical response and this must be accounted for in the design of optical devices.


Author(s):  
Sai Prasad Nayak ◽  
Venkatesh Srinivasan ◽  
Pradeep Kumar Badiya ◽  
J.K.Kiran Kumar ◽  
Sai Sathish Ramamurthy

2004 ◽  
Vol 324 (2) ◽  
pp. 170-182 ◽  
Author(s):  
Ignacy Gryczynski ◽  
Joanna Malicka ◽  
Zygmunt Gryczynski ◽  
Joseph R Lakowicz

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