In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis

2004 ◽  
Vol 76 (18) ◽  
pp. 5387-5392 ◽  
Author(s):  
Shaun D. Mendonsa ◽  
Michael T. Bowser
Keyword(s):  
Capillary Electrophoresis ◽  
In Vitro Selection ◽  
High Affinity ◽  
Selection Of

In vitro selection of high affinity HspR-binding sites within the genome of Helicobacter pylori

Gene ◽  
2002 ◽  
Vol 283 (1-2) ◽  
pp. 63-69 ◽  
Author(s):  
Isabel Delany ◽  
Gunther Spohn ◽  
Rino Rappuoli ◽  
Vincenzo Scarlato
Keyword(s):  
Helicobacter Pylori ◽  
Binding Sites ◽  
In Vitro Selection ◽  
High Affinity ◽  
Selection Of

In vitro selection of high-affinity nucleic acid ligands to parasite target molecules

2003 ◽  
Vol 33 (12) ◽  
pp. 1309-1317 ◽  
Author(s):  
H.Ulrich Göringer ◽  
Matthias Homann ◽  
Mihaela Lorger
Keyword(s):  
Nucleic Acid ◽  
In Vitro Selection ◽  
High Affinity ◽  
Target Molecules ◽  
Selection Of

scholarly journals Antibody-like proteins that capture and neutralize SARS-CoV-2

2020 ◽  
Vol 6 (42) ◽  
pp. eabd3916 ◽  
Author(s):  
T. Kondo ◽  
Y. Iwatani ◽  
K. Matsuoka ◽  
T. Fujino ◽  
S. Umemoto ◽  
...  
Keyword(s):  
High Speed ◽  
In Vitro Selection ◽  
Nasal Swab ◽  
Inhibitory Concentration ◽  
Rapid Development ◽  
Spike Protein ◽  
Emerging Pathogens ◽  
High Affinity ◽  
Selection Of

To combat severe acute respiratory syndrome–related coronavirus 2 (SARS-CoV-2) and any unknown emerging pathogens in the future, the development of a rapid and effective method to generate high-affinity antibodies or antibody-like proteins is of critical importance. We here report high-speed in vitro selection of multiple high-affinity antibody-like proteins against various targets including the SARS-CoV-2 spike protein. The sequences of monobodies against the SARS-CoV-2 spike protein were successfully procured within only 4 days. Furthermore, the obtained monobody efficiently captured SARS-CoV-2 particles from the nasal swab samples of patients and exhibited a high neutralizing activity against SARS-CoV-2 infection (half-maximal inhibitory concentration, 0.5 nanomolar). High-speed in vitro selection of antibody-like proteins is a promising method for rapid development of a detection method for, and of a neutralizing protein against, a virus responsible for an ongoing, and possibly a future, pandemic.

In vitro selection of a high affinity antibody to oestradiol using a phage display human antibody library

1996 ◽  
Vol 2 (3) ◽  
pp. 209-217 ◽  
Author(s):  
Anthony Pope ◽  
Kevin Pritchard ◽  
Andrew Williams ◽  
Andrew Roberts ◽  
John R. Hackett ◽  
...  
Keyword(s):  
Phage Display ◽  
In Vitro Selection ◽  
Human Antibody ◽  
Antibody Library ◽  
High Affinity ◽  
Selection Of
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