Investigation into the Applicability of the Centrifugal Microfluidics Platform for the Development of Protein−Ligand Binding Assays Incorporating Enhanced Green Fluorescent Protein as a Fluorescent Reporter

2004 ◽  
Vol 76 (24) ◽  
pp. 7263-7268 ◽  
Author(s):  
Libby G. Puckett ◽  
Emre Dikici ◽  
Siyi Lai ◽  
Marc Madou ◽  
Leonidas G. Bachas ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Ligand Binding ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Centrifugal Microfluidics ◽  
Binding Assays ◽  
Fluorescent Reporter ◽  
Green Fluorescent ◽  
Microfluidics Platform

scholarly journals Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

2006 ◽  
Vol 14 (21) ◽  
pp. 9815 ◽  
Author(s):  
Alberto Diaspro ◽  
Silke Krol ◽  
Barbara Campanini ◽  
Fabio Cannone ◽  
Giuseppe Chirico
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Gfp Fluorescence ◽  
Green Fluorescent

scholarly journals Development and Characterization of a cDNA-Launch Recombinant Simian Hemorrhagic Fever Virus Expressing Enhanced Green Fluorescent Protein: ORF 2b’ Is Not Required for In Vitro Virus Replication

Viruses ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 632
Author(s):  
Yingyun Cai ◽  
Shuiqing Yu ◽  
Ying Fang ◽  
Laura Bollinger ◽  
Yanhua Li ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Cell Lines ◽  
Enhanced Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Infectious Clone ◽  
Hemorrhagic Fever ◽  
Simian Hemorrhagic Fever Virus ◽  
Hemorrhagic Fever Virus ◽  
Green Fluorescent

Simian hemorrhagic fever virus (SHFV) causes acute, lethal disease in macaques. We developed a single-plasmid cDNA-launch infectious clone of SHFV (rSHFV) and modified the clone to rescue an enhanced green fluorescent protein-expressing rSHFV-eGFP that can be used for rapid and quantitative detection of infection. SHFV has a narrow cell tropism in vitro, with only the grivet MA-104 cell line and a few other grivet cell lines being susceptible to virion entry and permissive to infection. Using rSHFV-eGFP, we demonstrate that one cricetid rodent cell line and three ape cell lines also fully support SHFV replication, whereas 55 human cell lines, 11 bat cell lines, and three rodent cells do not. Interestingly, some human and other mammalian cell lines apparently resistant to SHFV infection are permissive after transfection with the rSHFV-eGFP cDNA-launch plasmid. To further demonstrate the investigative potential of the infectious clone system, we introduced stop codons into eight viral open reading frames (ORFs). This approach suggested that at least one ORF, ORF 2b’, is dispensable for SHFV in vitro replication. Our proof-of-principle experiments indicated that rSHFV-eGFP is a useful tool for illuminating the understudied molecular biology of SHFV.

Enhanced green fluorescent protein as a marker for localizing murine cytomegalovirus in acute and latent infection

2000 ◽  
Vol 89 (1-2) ◽  
pp. 61-73 ◽  
Author(s):  
Stanley C Henry ◽  
Kenneth Schmader ◽  
Talmage T Brown ◽  
Sara E Miller ◽  
David N Howell ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Enhanced Green Fluorescent Protein ◽  
Latent Infection ◽  
Fluorescent Protein ◽  
Murine Cytomegalovirus ◽  
Green Fluorescent
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