Label-Free Reading of Microarray-Based Immunoassays with Surface Plasmon Resonance Imaging

2004 ◽  
Vol 76 (24) ◽  
pp. 7257-7262 ◽  
Author(s):  
Vishal Kanda ◽  
James K. Kariuki ◽  
D. Jed Harrison ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

scholarly journals Rapid and Label-Free Immunosensing of Shiga Toxin Subtypes with Surface Plasmon Resonance Imaging

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 280
Author(s):  
Bin Wang ◽  
Bosoon Park ◽  
Jing Chen ◽  
Xiaohua He
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
High Throughput ◽  
Plasmon Resonance ◽  
Shiga Toxin ◽  
The United States ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Shiga Toxins

Shiga toxin-producing Escherichia coli (STEC) are responsible for gastrointestinal diseases reported in numerous outbreaks around the world as well as in the United States. Current detection methods have limitation to implement for rapid field-deployable detection with high volume of samples that are needed for regulatory purposes. Surface plasmon resonance imaging (SPRi) has proved to achieve rapid and label-free screening of multiple pathogens simultaneously, so it was evaluated in this work for the detection of Shiga toxins (Stx1a and Stx2a toxoids were used as the less toxic alternatives to Stx1 and Stx2, respectively). Multiple antibodies (Stx1pAb, Stx1-1mAb, Stx1-2mAb, Stx1d-3mAb, Stx1e-4mAb, Stx2pAb, Stx2-1mAb, Stx2-2mAb, and Stx2-10mAb) were spotted one by one by programed microarrayer, on the same high-throughput biochip with 50-nm gold film through multiple crosslinking and blocking steps to improve the orientation of antibodies on the biochip surface. Shiga toxins were detected based on the SPRi signal difference (ΔR) between immobilized testing antibodies and immunoglobulin G (IgG) control. Among the antibodies tested, Stx1pAb showed the highest sensitivity for Stx1 toxoid, with the limit of detection (LOD) of 50 ng/mL and detection time of 20 min. Both Stx2-1mAb and Stx2-2mAb exhibited high sensitivity for Stx2 toxoid. Furthermore, gold nanoparticles (GNPs) were used to amplify the SPRi signals of monoclonal antibodies in a sandwich platform. The LOD reached the level of picogram (pg)/mL with the help of GNP-antibody conjugate. This result proved that SPRi biochip with selected antibodies has the potential for rapid, high-throughput and multiplex detection of Shiga toxins.

A label-free approach to kinetic analysis and high multiplex detection of targeted drugs with phase surface plasmon resonance imaging

2015 ◽  
Vol 7 (5) ◽  
pp. 1738-1744 ◽  
Author(s):  
Yijia Wang ◽  
Chonglei Zhang ◽  
Yuquan Zhang ◽  
Hui Fang ◽  
Changjun Min ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Targeted Drugs ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Multiplex Detection ◽  
Resonance Imaging ◽  
Binding Reaction ◽  
Phase Surface

Phase SPRi biosensor with ability of high multiplex detection was applied to monitor binding reaction on different regions of chip.

SPRI biosensors for quantitative determination of matrix metalloproteinase-2

2017 ◽  
Vol 9 (16) ◽  
pp. 2407-2414 ◽  
Author(s):  
Anna Tokarzewicz ◽  
Lech Romanowicz ◽  
Iosif Sveklo ◽  
Ewa Matuszczak ◽  
Adam Hermanowicz ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Matrix Metalloproteinase ◽  
Quantitative Determination ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Matrix Metalloproteinase 2 ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging

The aim of this study was to develop a new, label-free, highly selective Surface Plasmon Resonance Imaging biosensor for the quantitative determination of matrix metalloproteinase-2.

Sign in / Sign up

Export Citation Format

Share Document