Metal Ion Complexes of EDTA:  A Solute System for Density Gradient Ultracentrifugation Analysis of Lipoproteins

2005 ◽  
Vol 77 (1) ◽  
pp. 200-207 ◽  
Author(s):  
Brian D. Hosken ◽  
Steven L. Cockrill ◽  
Ronald D. Macfarlane
Keyword(s):  
Density Gradient ◽  
Metal Ion ◽  
Density Gradient Ultracentrifugation ◽  
Metal Ion Complexes

Metal Ion Complexes of EDTA as Solutes for Density Gradient Ultracentrifugation:  Influence of Metal Ions

2005 ◽  
Vol 77 (21) ◽  
pp. 7054-7061 ◽  
Author(s):  
Jeffery D. Johnson ◽  
Natalie J. Bell ◽  
Erin L. Donahoe ◽  
Ronald D. Macfarlane
Keyword(s):  
Metal Ions ◽  
Density Gradient ◽  
Metal Ion ◽  
Density Gradient Ultracentrifugation ◽  
Metal Ion Complexes

Cleavage and isomerisation of UpU promoted by dinuclear metal ion complexes

2008 ◽  
Author(s):  
Heidi Linjalahti ◽  
Guoqiang Feng ◽  
Juan C. Mareque-Rivas ◽  
Satu Mikkola ◽  
Nicholas H. Williams
Keyword(s):  
Metal Ion ◽  
Metal Ion Complexes

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

Molecular dynamics of macrocycle-metal ion complexes involving heteronuclear binding atoms

1989 ◽  
Vol 61 (9) ◽  
pp. 1593-1596 ◽  
Author(s):  
L. J. Rodriguez ◽  
M. Xu ◽  
E. M. Eyring ◽  
S. Petrucci
Keyword(s):  
Molecular Dynamics ◽  
Metal Ion ◽  
Metal Ion Complexes

New divalent metal ion complexes with 1,8-diaminonapthalene -2-thione: Synthesis, Spectroscopic, anti-bacterial and anticancer activity studies

2021 ◽  
pp. 131291
Author(s):  
Ahmed S. Faihan ◽  
Mohammad R. Hatshan ◽  
Ali S. Alqahtani ◽  
Fahd A. Nasr ◽  
Subhi A. Al-Jibori ◽  
...  
Keyword(s):  
Anticancer Activity ◽  
Metal Ion ◽  
Divalent Metal ◽  
Metal Ion Complexes ◽  
Divalent Metal Ion
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