Development of a Bioluminescence Resonance Energy-Transfer Assay for Estrogen-Like Compound in Vivo Monitoring

2004 ◽  
Vol 76 (23) ◽  
pp. 7069-7076 ◽  
Author(s):  
Elisa Michelini ◽  
Mara Mirasoli ◽  
Matti Karp ◽  
Marko Virta ◽  
Aldo Roda
Keyword(s):  
Energy Transfer ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
In Vivo Monitoring

scholarly journals Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo

2016 ◽  
Vol 52 (43) ◽  
pp. 6997-7000 ◽  
Author(s):  
Anyanee Kamkaew ◽  
Haiyan Sun ◽  
Christopher G. England ◽  
Liang Cheng ◽  
Zhuang Liu ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Lymph Node ◽  
Quantum Dot ◽  
Tumor Imaging ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
Lymph Node Mapping

A small luciferase (Nluc) was conjugated to QDs as a bioluminescence resonance energy transfer (BRET) pair.

scholarly journals An enhanced molecular tension sensor based on bioluminescence resonance energy transfer (BRET)

2019 ◽  
Author(s):  
Eric J. Aird ◽  
Kassidy J. Tompkins ◽  
Wendy R. Gordon
Keyword(s):  
Energy Transfer ◽  
Dynamic Range ◽  
Resonance Energy Transfer ◽  
Focal Adhesions ◽  
Resonance Energy ◽  
Cellular Protein ◽  
Bioluminescence Resonance Energy Transfer ◽  
Tension Sensor ◽  
Focal Adhesion Protein

ABSTRACTMolecular tension sensors measure piconewton forces experienced by individual proteins in the context of the cellular microenvironment. Current genetically-encoded tension sensors use FRET to report on extension of an elastic peptide encoded in a cellular protein of interest. Here we present the development and characterization of a new type of molecular tension sensor based on bioluminescence resonance energy transfer (BRET) which exhibits more desirable spectral properties and an enhanced dynamic range compared to other molecular tension sensors. Moreover, it avoids many disadvantages of FRET measurements in cells, including heating of the sample, autofluorescence, photobleaching, and corrections of direct acceptor excitation. We benchmark the sensor by inserting it into the canonical mechanosensing focal adhesion protein vinculin, observing highly resolved gradients of tensional changes across focal adhesions. We anticipate that the BRET-TS will expand the toolkit available to study mechanotransduction at a molecular level and allow potential extension to an in vivo context.

scholarly journals Self-illuminating in vivo lymphatic imaging using a bioluminescence resonance energy transfer quantum dot nano-particle

2010 ◽  
Vol 6 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Nobuyuki Kosaka ◽  
Makoto Mitsunaga ◽  
Sukanta Bhattacharyya ◽  
Steven C. Miller ◽  
Peter L. Choyke ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Nano Particle ◽  
Bioluminescence Resonance Energy Transfer ◽  
Lymphatic Imaging

scholarly journals Integration of Nanomaterials and Bioluminescence Resonance Energy Transfer Techniques for Sensing Biomolecules

Biosensors ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 42 ◽  
Author(s):  
Eugene Hwang ◽  
Jisu Song ◽  
Jin Zhang
Keyword(s):  
Quantum Dots ◽  
Energy Transfer ◽  
Uv Light ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
External Electromagnetic Field ◽  
Ease Of Use ◽  
Radiative Energy ◽  
Bioluminescence Resonance Energy Transfer

Bioluminescence resonance energy transfer (BRET) techniques offer a high degree of sensitivity, reliability and ease of use for their application to sensing biomolecules. BRET is a distance dependent, non-radiative energy transfer, which uses a bioluminescent protein to excite an acceptor through the resonance energy transfer. A BRET sensor can quickly detect the change of a target biomolecule quantitatively without an external electromagnetic field, e.g., UV light, which normally can damage tissue. Having been developed quite recently, this technique has evolved rapidly. Here, different bioluminescent proteins have been reviewed. In addition to a multitude of bioluminescent proteins, this manuscript focuses on the recent development of BRET sensors by utilizing quantum dots. The special size-dependent properties of quantum dots have made the BRET sensing technique attractive for the real-time monitoring of the changes of target molecules and bioimaging in vivo. This review offers a look into the basis of the technique, donor/acceptor pairs, experimental applications and prospects.

A quantum-dot based protein module for in vivo monitoring of protease activity through fluorescence resonance energy transfer

2011 ◽  
Vol 47 (18) ◽  
pp. 5259 ◽  
Author(s):  
Payal Biswas ◽  
Lakshmi N. Cella ◽  
Seung Hyun Kang ◽  
Ashok Mulchandani ◽  
Marylynn V. Yates ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Quantum Dot ◽  
Fluorescence Resonance Energy Transfer ◽  
Protease Activity ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
In Vivo Monitoring ◽  
Fluorescence Resonance

scholarly journals Multi-resolution imaging using bioluminescence resonance energy transfer identifies distinct biodistribution profiles of extracellular vesicles and exomeres with redirected tropism

2020 ◽  
Author(s):  
Anthony Yan-Tang Wu ◽  
Yun-Chieh Sung ◽  
Yen-Ju Chen ◽  
Steven Ting-Yu Chou ◽  
Vanessa Guo ◽  
...  
Keyword(s):  
Energy Transfer ◽  
Extracellular Vesicles ◽  
Organic Anion ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Bioluminescence Resonance Energy Transfer ◽  
Anion Transporter ◽  
Transporter Family ◽  
Starting Point

AbstractExtracellular particles (EP) including extracellular vesicles (EVs) and exomeres have been shown to play significant roles in diseases and therapeutic applications. However, their spatiotemporal dynamics in vivo have remained largely unresolved in detail due to the lack of a suitable method. We therefore created a bioluminescence resonance energy transfer (BRET)-based reporter, PalmGRET, to enable pan-EP labelling ranging from exomeres (< 50 nm) to small (< 200 nm) and medium and large (> 200 nm) EVs. PalmGRET emits robust, sustained signals and allows the visualization, tracking and quantification of the EPs from whole-animal to nanoscopic resolutions under different imaging modalities, including bioluminescence, BRET and fluorescence. Using PalmGRET, we show that EPs released by lung metastatic hepatocellular carcinoma (HCC) exhibit lung tropism with varying distributions to other major organs in immunocompetent mice. We further demonstrate that gene knockdown of lung-tropic membrane proteins, solute carrier organic anion transporter family member 2A1 (Slco2a1), alanine aminopeptidase (Cd13) and chloride intracellular channel (Clic1) decreases HCC-EP distribution to the lungs and yields distinct biodistribution profiles. We anticipate that EP-specific imaging, quantitative assays and detailed in vivo characterization to be a starting point for more accurate and comprehensive in vivo models of EP biology and therapeutic design.

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