Capillary Electrophoresis and Fluorescence Anisotropy for Quantitative Analysis of Peptide−Protein Interactions Using JAK2 and SH2-Bβ as a Model System

2005 ◽  
Vol 77 (8) ◽  
pp. 2482-2489 ◽  
Author(s):  
Peilin Yang ◽  
Rebecca J. Whelan ◽  
Emily E. Jameson ◽  
Jason H. Kurzer ◽  
Lawrence S. Argetsinger ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Analysis ◽  
Protein Interactions ◽  
Fluorescence Anisotropy ◽  
Model System

Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary Electrophoresis

2011 ◽  
Vol 83 (16) ◽  
pp. 6196-6201 ◽  
Author(s):  
Nasrin Khan ◽  
Jenny Cheng ◽  
John Paul Pezacki ◽  
Maxim V. Berezovski
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Analysis ◽  
Blood Serum ◽  
Affinity Capillary Electrophoresis

Quantitative Analysis of Corn Zeins by Capillary Electrophoresis

1997 ◽  
Vol 74 (6) ◽  
pp. 766-770 ◽  
Author(s):  
Nicholas Parris ◽  
Leland Dickey ◽  
James Craig
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Analysis

scholarly journals Mobility Shift Affinity Capillary Electrophoresis at High Ligand Concentrations: Application to Aluminum Chlorohydrate–Protein Interactions

ACS Omega ◽  
2018 ◽  
Vol 3 (12) ◽  
pp. 17547-17554
Author(s):  
Nesrine Ouadah ◽  
Claudine Moire ◽  
Fabien Brothier ◽  
Jean-François Kuntz ◽  
Michal Malý ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Protein Interactions ◽  
Affinity Capillary Electrophoresis ◽  
Mobility Shift ◽  
High Ligand

Characterization of basic drug–human serum protein interactions by capillary electrophoresis

2006 ◽  
Vol 27 (17) ◽  
pp. 3410-3419 ◽  
Author(s):  
María A. Martínez-Gómez ◽  
Salvador Sagrado ◽  
Rosa M. Villanueva-Camañas ◽  
Maria J. Medina-Hernández
Keyword(s):  
Capillary Electrophoresis ◽  
Human Serum ◽  
Serum Protein ◽  
Protein Interactions ◽  
Basic Drug ◽  
Human Serum Protein

scholarly journals A rapid and reliable CE-LIF method for the quantitative analysis of miRNA-497 in plasma and organs and its application to a pharmacokinetic and biodistribution study

RSC Advances ◽  
2020 ◽  
Vol 10 (32) ◽  
pp. 18648-18654
Author(s):  
Eunmi Ban ◽  
Haejin Kwon ◽  
Eun Joo Song
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Analysis ◽  
Laser Induced Fluorescence ◽  
Biodistribution Study

This study evaluated the pharmacokinetic and biodistribution of miRNA-497 mimic as model miRNA for lung target using capillary electrophoresis with laser-induced fluorescence.

Quantitative analysis of propofol in whole blood using capillary electrophoresis

2009 ◽  
Vol 877 (8-9) ◽  
pp. 703-709 ◽  
Author(s):  
Yu Hui ◽  
Koen Raedschelders ◽  
Hong Zhang ◽  
David M. Ansley ◽  
David D.Y. Chen
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Analysis ◽  
Whole Blood

scholarly journals Saccharomyces cerevisiae as a Tool to Investigate Plant Potassium and Sodium Transporters

2019 ◽  
Vol 20 (9) ◽  
pp. 2133 ◽  
Author(s):  
Antonella Locascio ◽  
Nuria Andrés-Colás ◽  
José Miguel Mulet ◽  
Lynne Yenush
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Interactions ◽  
Model System ◽  
Protein Protein Interactions ◽  
Alkali Cations ◽  
Yeast Saccharomyces Cerevisiae ◽  
Future Directions ◽  
Sodium Transporters ◽  
Adverse Environmental Conditions ◽  
Sodium And Potassium

Sodium and potassium are two alkali cations abundant in the biosphere. Potassium is essential for plants and its concentration must be maintained at approximately 150 mM in the plant cell cytoplasm including under circumstances where its concentration is much lower in soil. On the other hand, sodium must be extruded from the plant or accumulated either in the vacuole or in specific plant structures. Maintaining a high intracellular K+/Na+ ratio under adverse environmental conditions or in the presence of salt is essential to maintain cellular homeostasis and to avoid toxicity. The baker’s yeast, Saccharomyces cerevisiae, has been used to identify and characterize participants in potassium and sodium homeostasis in plants for many years. Its utility resides in the fact that the electric gradient across the membrane and the vacuoles is similar to plants. Most plant proteins can be expressed in yeast and are functional in this unicellular model system, which allows for productive structure-function studies for ion transporting proteins. Moreover, yeast can also be used as a high-throughput platform for the identification of genes that confer stress tolerance and for the study of protein–protein interactions. In this review, we summarize advances regarding potassium and sodium transport that have been discovered using the yeast model system, the state-of-the-art of the available techniques and the future directions and opportunities in this field.

Micropatterning for quantitative analysis of protein-protein interactions in living cells

2008 ◽  
Vol 5 (12) ◽  
pp. 1053-1060 ◽  
Author(s):  
Michaela Schwarzenbacher ◽  
Martin Kaltenbrunner ◽  
Mario Brameshuber ◽  
Clemens Hesch ◽  
Wolfgang Paster ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Protein Interactions ◽  
Living Cells ◽  
Protein Protein Interactions
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