Capillary Electrophoresis Chips with a Sheath-Flow Supported Electrochemical Detection System

2004 ◽  
Vol 76 (13) ◽  
pp. 3749-3755 ◽  
Author(s):  
Peter Ertl ◽  
Charles A. Emrich ◽  
Pankaj Singhal ◽  
Richard A. Mathies
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
Detection System ◽  
Sheath Flow

Capillary electrophoresis/electrochemical detection system with on-line deoxygenation

2000 ◽  
Vol 21 (8) ◽  
pp. 1540-1544 ◽  
Author(s):  
Wenrui Jin ◽  
Qian Dong ◽  
Daiqing Yu ◽  
Xiaoying Ye ◽  
Wei Li
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection ◽  
Detection System ◽  
On Line

scholarly journals DNA tetrahedron-mediated immune-sandwich assay for rapid and sensitive detection of PSA through a microfluidic electrochemical detection system

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Dezhi Feng ◽  
Jing Su ◽  
Yi Xu ◽  
Guifang He ◽  
Chenguang Wang ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Reaction Time ◽  
Electrochemical Detection ◽  
Detection System ◽  
Detection Performance ◽  
Pdms Layer ◽  
Microfluidic Chips ◽  
Serum Samples ◽  
Gold Nanoflowers ◽  
Dna Tetrahedron

AbstractProstate-specific antigen (PSA) is the most widely used biomarker for the early diagnosis of prostate cancer. Existing methods for PSA detection are burdened with some limitations and require improvement. Herein, we developed a novel microfluidic–electrochemical (μFEC) detection system for PSA detection. First, we constructed an electrochemical biosensor based on screen-printed electrodes (SPEs) with modification of gold nanoflowers (Au NFs) and DNA tetrahedron structural probes (TSPs), which showed great detection performance. Second, we fabricated microfluidic chips by DNA TSP-Au NF-modified SPEs and a PDMS layer with designed dense meandering microchannels. Finally, the μFEC detection system was achieved based on microfluidic chips integrated with the liquid automatic conveying unit and electrochemical detection platform. The μFEC system we developed acquired great detection performance for PSA detection in PBS solution. For PSA assays in spiked serum samples of the μFEC system, we obtained a linear dynamic range of 1–100 ng/mL with a limit of detection of 0.2 ng/mL and a total reaction time <25 min. Real serum samples of prostate cancer patients presented a strong correlation between the “gold-standard” chemiluminescence assays and the μFEC system. In terms of operation procedure, cost, and reaction time, our method was superior to the current methods for PSA detection and shows great potential for practical clinical application in the future.

Application of Diamond Microelectrodes for End-Column Electrochemical Detection in Capillary Electrophoresis

2003 ◽  
Vol 75 (3) ◽  
pp. 530-534 ◽  
Author(s):  
Dongchan Shin ◽  
Bulusu V. Sarada ◽  
Donald A. Tryk ◽  
Akira Fujishima ◽  
Joseph Wang
Keyword(s):  
Capillary Electrophoresis ◽  
Electrochemical Detection
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