Microfluidic Chip for Continuous Monitoring of Hormone Secretion from Live Cells Using an Electrophoresis-Based Immunoassay

2003 ◽  
Vol 75 (18) ◽  
pp. 4711-4717 ◽  
Author(s):  
Michael G. Roper ◽  
Jonathan G. Shackman ◽  
Gabriella M. Dahlgren ◽  
Robert T. Kennedy
Keyword(s):  
Microfluidic Chip ◽  
Continuous Monitoring ◽  
Hormone Secretion ◽  
Live Cells

Continuous monitoring of EDTA extractable iron from mineral slurries using a microfluidic chip

2021 ◽  
Author(s):  
Daisy Yang ◽  
Aliaa I. Shallan ◽  
Michael C. Breadmore ◽  
Christopher Greet ◽  
Craig Priest
Keyword(s):  
Microfluidic Chip ◽  
Continuous Monitoring ◽  
Extractable Iron

Modular Microfluidic Filters Based on Transparent Membranes

2016 ◽  
Vol 138 (4) ◽  
Author(s):  
E. Archibong ◽  
H. Tuazon ◽  
H. Wang ◽  
J. Winskas ◽  
A. L. Pyayt
Keyword(s):  
Microfluidic Chip ◽  
Membrane Damage ◽  
Particle Separation ◽  
Live Cells ◽  
New Approach ◽  
Silicon Nitride Membrane ◽  
Particle Imaging ◽  
Silicon Based ◽  
Functional Components ◽  
Low Pressures

We propose a new approach to the modular packaging of microfluidic components, in which different functional components are not only fabricated separately but are also designed to be individually removable for the purposes of replacement or subsequent analysis. In this paper, we demonstrate one such component: a stand-alone microfluidic filter that can be custom-fabricated and then connected, disconnected, and replaced on a microfluidic chip as needed. This filter is also designed such that particles captured on the filter can be further analyzed or processed directly on the filter itself—for example, for microscopic examination or cell culturing. The filter is a thin (1 μm) transparent silicon nitride membrane that can be designed and fabricated according to specifications for different applications. This material is suitable for microscale fabrication; filtration of a variety of solutions, including biological samples; and subsequent particle imaging and processing. The porous nature of the thin filter allows for particle separation under relatively low pressures, thus protecting the particles from rupture or membrane damage. We describe two methods for integrating the filter apparatus onto a microfluidic chip such that it can be inserted, removed, and replaced. To demonstrate the utility of this approach, we fabricated custom-designed silicon-based filters, incorporated them onto microfluidic systems then filtered microparticles and live cells from test solutions, and finally removed the filters to image the microparticles and culture the cells directly on the filter membranes.

4-dimensional, high-resolution imaging of living cells

1992 ◽  
Vol 50 (1) ◽  
pp. 416-417
Author(s):  
Shinya Inoué
Keyword(s):  
Light Microscope ◽  
Living Cells ◽  
Live Cells ◽  
Video Tape ◽  
Active Living ◽  
High Resolution Imaging ◽  
3 Dimensional ◽  
Dynamic Architecture ◽  
Resolution Imaging ◽  
Disk Memory

This paper reports progress of our effort to rapidly capture, and display in time-lapsed mode, the 3-dimensional dynamic architecture of active living cells and developing embryos at the highest resolution of the light microscope. Our approach entails: (A) real-time video tape recording of through-focal, ultrathin optical sections of live cells at the highest resolution of the light microscope; (B) repeat of A at time-lapsed intervals; (C) once each time-lapsed interval, an image at home focus is recorded onto Optical Disk Memory Recorder (OMDR); (D) periods of interest are selected using the OMDR and video tape records; (E) selected stacks of optical sections are converted into plane projections representing different view angles (±4 degrees for stereo view, additional angles when revolving stereos are desired); (F) analysis using A - D.

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

Characterization of a Motile Cellular Domain Arising During the Amoebo-Flagellate Transformation of Physarum Polycephalum

1980 ◽  
Vol 38 ◽  
pp. 552-553
Author(s):  
K.I. Pagh ◽  
M.R. Adelman
Keyword(s):  
Cell Shape ◽  
Physarum Polycephalum ◽  
Slime Mold ◽  
Live Cells ◽  
Cellular Domain

Unicellular amoebae of the slime mold Physarum polycephalum undergo marked changes in cell shape and motility during their conversion into flagellate swimming cells (l). To understand the processes underlying motile activities expressed during the amoebo-flagellate transformation, we have undertaken detailed investigations of the organization, formation and functions of subcellular structures or domains of the cell which are hypothesized to play a role in movement. One focus of our studies is on a structure, termed the “ridge” which appears as a flattened extension of the periphery along the length of transforming cells (Fig. 1). Observations of live cells using Nomarski optics reveal two types of movement in this region:propagation of undulations along the length of the ridge and formation and retraction of filopodial projections from its edge. The differing activities appear to be associated with two characteristic morphologies, illustrated in Fig. 1.

The multicompartment SlideReactor – a novel concept for continuous monitoring of co-cultured cells

2009 ◽  
Vol 47 (01) ◽  
Author(s):  
N Billecke ◽  
S Tröller ◽  
N Raschzok ◽  
MH Morgül ◽  
NN Kammer ◽  
...  
Keyword(s):  
Continuous Monitoring ◽  
Cultured Cells ◽  
Novel Concept
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