Ion Sensing and Inhibition Studies Using the Transmembrane Ion Channel Peptide Gramicidin A Entrapped in Sol−Gel-Derived Silica

2003 ◽  
Vol 75 (5) ◽  
pp. 1094-1101 ◽  
Author(s):  
Travis R. Besanger ◽  
John D. Brennan
Keyword(s):  
Ion Channel ◽  
Sol Gel ◽  
Gramicidin A ◽  
Ion Sensing ◽  
Inhibition Studies

Neutral-Carrier-Type Ion-Sensing Membranes Based on Sol-Gel-Derived Glass

1995 ◽  
Vol 24 (10) ◽  
pp. 967-967 ◽  
Author(s):  
Keiichi Kimura ◽  
Takenobu Sunagawa ◽  
Masaaki Yokoyama
Keyword(s):  
Sol Gel ◽  
Neutral Carrier ◽  
Ion Sensing

scholarly journals Redox-regulated ion channel activity of a cysteine-containing gramicidin A analogue

2006 ◽  
Vol 1758 (4) ◽  
pp. 493-498 ◽  
Author(s):  
Yuri N. Antonenko ◽  
Tatyana B. Stoilova ◽  
Sergey I. Kovalchuk ◽  
Natalya S. Egorova ◽  
Alina A. Pashkovskaya ◽  
...  
Keyword(s):  
Ion Channel ◽  
Gramicidin A ◽  
Channel Activity

Chemically Reactive Derivatives of Gramicidin A for Developing Ion Channel-Based Nanoprobes

2008 ◽  
Vol 19 (8) ◽  
pp. 1614-1624 ◽  
Author(s):  
Steven Blake ◽  
Ricardo Capone ◽  
Michael Mayer ◽  
Jerry Yang
Keyword(s):  
Ion Channel ◽  
Gramicidin A ◽  
Derivatives Of

scholarly journals Effects of CF4 Plasma Treatment on Indium Gallium Oxide and Ti-doped Indium Gallium Oxide Sensing Membranes in Electrolyte–Insulator–Semiconductors

Crystals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 810
Author(s):  
Chyuan-Haur Kao ◽  
Yen-Lin Su ◽  
Wei-Jen Liao ◽  
Ming-Hsien Li ◽  
Wei-Lun Chan ◽  
...  
Keyword(s):  
Plasma Treatment ◽  
Gallium Oxide ◽  
Sol Gel ◽  
Chemical Vapor ◽  
High Sensitivity ◽  
Ph Sensing ◽  
Ion Sensing ◽  
Indium Gallium ◽  
Optical Applications ◽  
Novel Design

Electrolyte–insulator–semiconductor (EIS) sensors, used in applications such as pH sensing and sodium ion sensing, are the most basic type of ion-sensitive field-effect transistor (ISFET) membranes. Currently, some of the most popular techniques for synthesizing such sensors are chemical vapor deposition, reactive sputtering and sol-gel deposition. However, there are certain limitations on such techniques, such as reliability concerns and isolation problems. In this research, a novel design of an EIS membrane consisting of an optical material of indium gallium oxide (IGO) was demonstrated. Compared with conventional treatment such as annealing, Ti doping and CF4 plasma treatment were incorporated in the fabrication of the film. Because of the effective treatment of doping and plasma treatment, the defects were mitigated and the membrane capacitance was boosted. Therefore, the pH sensitivity can be increased up to 60.8 mV/pH. In addition, the hysteresis voltage can be improved down to 2.1 mV, and the drift voltage can be suppressed to as low as 0.23 mV/h. IGO-based membranes are promising for future high-sensitivity and -stability devices integrated with optical applications.

An asymmetric ion channel derived from gramicidin A

FEBS Journal ◽  
2005 ◽  
Vol 272 (4) ◽  
pp. 975-986 ◽  
Author(s):  
Xiulan Xie ◽  
Lo'ay Al-Momani ◽  
Philipp Reiß ◽  
Christian Griesinger ◽  
Ulrich Koert
Keyword(s):  
Ion Channel ◽  
Gramicidin A

The divalent cation-binding sites of gramicidin A transmembrane ion-channel

Biopolymers ◽  
1991 ◽  
Vol 31 (4) ◽  
pp. 425-434 ◽  
Author(s):  
A. P. Golovanov ◽  
I. L. Barsukov ◽  
A. S. Arseniev ◽  
V. F. Bystrov ◽  
S. V. Sukhanov ◽  
...  
Keyword(s):  
Ion Channel ◽  
Divalent Cation ◽  
Binding Sites ◽  
Cation Binding ◽  
Gramicidin A

scholarly journals Discovery of gramicidin A analogues with altered activities by multidimensional screening of a one-bead-one-compound library

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Yuri Takada ◽  
Hiroaki Itoh ◽  
Atmika Paudel ◽  
Suresh Panthee ◽  
Hiroshi Hamamoto ◽  
...  
Keyword(s):  
Ion Channel ◽  
Large Scale ◽  
Antibacterial Activities ◽  
Gramicidin A ◽  
Ion Concentration ◽  
Peptide Antibiotic ◽  
Structure Activity ◽  
Common Ion ◽  
Multidimensional Screening ◽  
The Common

Abstract Gramicidin A (1) is a peptide antibiotic that disrupts the transmembrane ion concentration gradient by forming an ion channel in a lipid bilayer. Although long used clinically, it is limited to topical application because of its strong hemolytic activity and mammalian cytotoxicity, likely arising from the common ion transport mechanism. Here we report an integrated high-throughput strategy for discovering analogues of 1 with altered biological activity profiles. The 4096 analogue structures are designed to maintain the charge-neutral, hydrophobic, and channel forming properties of 1. Synthesis of the analogues, tandem mass spectrometry sequencing, and 3 microscale screenings enable us to identify 10 representative analogues. Re-synthesis and detailed functional evaluations find that all 10 analogues share a similar ion channel function, but have different cytotoxic, hemolytic, and antibacterial activities. Our large-scale structure-activity relationship studies reveal the feasibility of developing analogues of 1 that selectively induce toxicity toward target organisms.

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