On-Chip Coupling of Isoelectric Focusing and Free Solution Electrophoresis for Multidimensional Separations

2003 ◽  
Vol 75 (5) ◽  
pp. 1180-1187 ◽  
Author(s):  
Amy E. Herr ◽  
Joshua I. Molho ◽  
Katerina A. Drouvalakis ◽  
James C. Mikkelsen ◽  
Paul J. Utz ◽  
...  
Keyword(s):  
Isoelectric Focusing ◽  
Free Solution ◽  
Multidimensional Separations ◽  
On Chip

The formation of stable pH gradients with weak monovalent buffers for isoelectric focusing in free solution

1985 ◽  
Vol 6 (11) ◽  
pp. 545-551 ◽  
Author(s):  
Richard A. Mosher ◽  
Wolfgang Thormann ◽  
Aly Graham ◽  
Milan Bier
Keyword(s):  
Isoelectric Focusing ◽  
Free Solution ◽  
Ph Gradients

Effects of ampholyte concentration on protein behavior in on-chip isoelectric focusing

2008 ◽  
Vol 29 (5) ◽  
pp. 1026-1035 ◽  
Author(s):  
Jaesool Shim ◽  
Prashanta Dutta ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
On Chip

Multistage Isoelectric Focusing: A Novel On-Chip Bio-Separation Technique

2005 ◽  
Author(s):  
Prashanta Dutta ◽  
Keisuke Horiuchi ◽  
Huanchun Cui ◽  
Cornelius F. Ivory
Keyword(s):  
Isoelectric Focusing ◽  
Microfluidic Chip ◽  
Soft Lithography ◽  
Fluorescent Protein ◽  
Ph Gradient ◽  
Resolving Power ◽  
Protein Species ◽  
Second Stage ◽  
On Chip ◽  
Bonding Technique

This experimental study reports a method to increase the resolving power of isoelectric focusing (IEF) on a polymeric microfluidic chip. Microfluidic chip is formed on poly-di-methyl siloxane (PDMS) using soft lithography and multilayer bonding technique. In this novel bioseparation technique, IEF is staged by first focusing protein species in a straight channel using broad-range ampholytes and then refocusing segments of that first channel into secondary channels that branch out from the first one. Experiments demonstrated that three fluorescent protein species within a segment of pH gradient in the first stage were refocused in the second stage with much higher resolution in a shallower pH gradient. A serially performed two-stage IEF was completed in less than 25 minutes under particularly small electric field strength up to 100 V/cm.

Two-Dimensional Isoelectric Focusing OFFGEL and Microfluidic Lab-on-Chip Electrophoresis for Assessing Dissolved Proteins in Seawater

2013 ◽  
Vol 85 (12) ◽  
pp. 5909-5916 ◽  
Author(s):  
Natalia García-Otero ◽  
Elena Peña-Vázquez ◽  
María Carmen Barciela-Alonso ◽  
Pilar Bermejo-Barrera ◽  
Antonio Moreda-Piñeiro
Keyword(s):  
Isoelectric Focusing ◽  
Two Dimensional ◽  
Lab On Chip ◽  
On Chip

On-chip technologies for multidimensional separations

Lab on a Chip ◽  
2009 ◽  
Vol 9 (17) ◽  
pp. 2524 ◽  
Author(s):  
Samuel Tia ◽  
Amy E. Herr
Keyword(s):  
Multidimensional Separations ◽  
On Chip
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