High-Resolution Colocalization of Single Dye Molecules by Fluorescence Lifetime Imaging Microscopy

2002 ◽  
Vol 74 (14) ◽  
pp. 3511-3517 ◽  
Author(s):  
Mike Heilemann ◽  
Dirk P. Herten ◽  
Rainer Heintzmann ◽  
Christoph Cremer ◽  
Christian Müller ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Lifetime ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Dye Molecules ◽  
Lifetime Imaging

scholarly journals Timing and Operating Mode Design for Time-Gated Fluorescence Lifetime Imaging Microscopy

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Chao Liu ◽  
Xinwei Wang ◽  
Yan Zhou ◽  
Yuliang Liu
Keyword(s):  
High Resolution ◽  
Fluorescence Imaging ◽  
Fluorescence Lifetime ◽  
Imaging Techniques ◽  
Absolute Measurement ◽  
Operating Mode ◽  
Least Square ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging

Steady-state fluorence imaging and time-resolved fluorescence imaging are two important areas in fluorescence imaging research. Fluorescence lifetime imaging is an absolute measurement method which is independent of excitation laser intensity, fluorophore concentration, and photobleaching compared to fluorescence intensity imaging techniques. Time-gated fluorescence lifetime imaging microscopy (FLIM) can provide high resolution and high imaging frame during mature FLIM methods. An abstract time-gated FLIM model was given, and important temporal parameters are shown as well. Aiming at different applications of steady and transient fluorescence processes, two different operation modes, timing and lifetime computing algorithm are designed. High resolution and high frame can be achieved by one-excitation one-sampling mode and least square algorithm for steady imaging applications. Correspondingly, one-excitation two-sampling mode and rapid lifetime determination algorithm contribute to transient fluorescence situations.

scholarly journals Direct organelle thermometry with fluorescence lifetime imaging microscopy in single myotubes

2016 ◽  
Vol 52 (24) ◽  
pp. 4458-4461 ◽  
Author(s):  
Hideki Itoh ◽  
Satoshi Arai ◽  
Thankiah Sudhaharan ◽  
Sung-Chan Lee ◽  
Young-Tae Chang ◽  
...  
Keyword(s):  
Fluorescence Lifetime ◽  
Heat Production ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
C2c12 Myotube

FLIM of ER thermo yellow and non-targeted mCherry reveals the Ca2+-dependent heat production localized to SR in C2C12 myotube.

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