Automated Gain Control and Internal Calibration with External Ion Accumulation Capillary Liquid Chromatography-Electrospray Ionization-Fourier Transform Ion Cyclotron Resonance

2003 ◽  
Vol 75 (16) ◽  
pp. 4195-4205 ◽  
Author(s):  
Mikhail E. Belov ◽  
Rui Zhang ◽  
Eric F. Strittmatter ◽  
David C. Prior ◽  
Keqi Tang ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Liquid Chromatography ◽  
Electrospray Ionization ◽  
Cyclotron Resonance ◽  
Gain Control ◽  
Capillary Liquid Chromatography ◽  
Ion Cyclotron Resonance ◽  
Internal Calibration ◽  
Electrospray Ionization Fourier Transform ◽  
Capillary Liquid

scholarly journals Micro-capillary liquid chromatography Fourier transform ion cyclotron resonance mass spectrometry--a powerful tool for peptide and protein identification.

2001 ◽  
Vol 48 (4) ◽  
pp. 1101-1104 ◽  
Author(s):  
M Ramström ◽  
M Palmblad ◽  
A Amirkhani ◽  
Y O Tsybin ◽  
K E Markides ◽  
...  
Keyword(s):  
Fourier Transform ◽  
Liquid Chromatography ◽  
Cyclotron Resonance ◽  
Protein Identification ◽  
Capillary Liquid Chromatography ◽  
Ion Cyclotron Resonance ◽  
Sequence Coverage ◽  
Tryptic Digest ◽  
Ion Cyclotron ◽  
Capillary Liquid

In order to be able to study complex biological samples, a micro-capillary liquid chromatography system was coupled to a 9.4 T Fourier transform ion cyclotron resonance mass spectrometer. The setup was tested on a tryptic digest of bovine serum albumin, which resulted in high sequence coverage (> 92%) of the protein.

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