Dual Conductivity/Amperometric Detection System for Microchip Capillary Electrophoresis

2002 ◽  
Vol 74 (23) ◽  
pp. 5919-5923 ◽  
Author(s):  
Joseph Wang ◽  
Martin Pumera
Keyword(s):  
Capillary Electrophoresis ◽  
Detection System ◽  
Amperometric Detection ◽  
Microchip Capillary Electrophoresis

Determination of Levoglucosan from Smoke Samples Using Microchip Capillary Electrophoresis with Pulsed Amperometric Detection

2005 ◽  
Vol 39 (2) ◽  
pp. 618-623 ◽  
Author(s):  
Carlos D. García ◽  
Guenter Engling ◽  
Pierre Herckes ◽  
Jeffrey L. Collett, ◽  
Charles S. Henry
Keyword(s):  
Capillary Electrophoresis ◽  
Amperometric Detection ◽  
Microchip Capillary Electrophoresis ◽  
Pulsed Amperometric Detection

Rapid Detection of AGs using Microchip Capillary Electrophoresis Contactless Conductivity Detection

2018 ◽  
Vol 15 (1) ◽  
pp. 9-16 ◽  
Author(s):  
Gangzhi Zhu ◽  
Chunjie Bao ◽  
Wenfang Liu ◽  
Xingxing Yan ◽  
Lili Liu ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Separation Efficiency ◽  
Detection System ◽  
Signal To Noise Ratio ◽  
Background Electrolyte ◽  
Microchip Capillary Electrophoresis ◽  
Conductivity Detection ◽  
Contactless Conductivity Detection ◽  
Kanamycin Sulfate ◽  
Ethanesulfonic Acid

Background: In order to realize current aminoglycosides supervision in food and environment, our team improved the sensitivity and separation efficiency of the portable ITO detector, based on the technology of microchip capillary electrophoresis and contactless conductivity detection. Experiment: Parameters (the separation voltage, buffer concentration, electrodes gap, elicitation frequency, elicitation voltage) were optimized for the detection of three aminoglycosides, gentamicin, kanamycin and streptomycin and the separation of their mixture in background electrolyte consists of 2-(N-Morpholino) ethanesulfonic acid (MES) and L-Histidine (His). The enhanced method was also applied to other types of aminoglycosides. Results: Under optimal conditions, the monitoring of three types of aminoglycosides obtained such a sensitive response that the limits of detection of gentamicin sulfate, kanamycin sulfate and streptomycin sulfate were calculated as 3.1 µg/ml, 0.89 µg/ml and 0.96 µg/ml, at signal-to-noise ratio 3, respectively. In addition they got separated completely from each other only in 40 s. The results of other varieties of aminoglycosides including tobramycin sulfate and amikacin sulfate also met the standard. Conclusion: We successfully proposed here an unprecedentedly portable, miniaturized and rapid microchip capillary electrophoresis contactless conductivity detection system to realize current aminoglycosides supervision in food and environment.

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