Label-Free Parallel Screening of Combinatorial Triazine Libraries Using Reflectometric Interference Spectroscopy

2002 ◽  
Vol 74 (4) ◽  
pp. 834-840 ◽  
Author(s):  
Oliver Birkert ◽  
Rolf Tünnemann ◽  
Günther Jung ◽  
Günter Gauglitz
Keyword(s):  
Label Free ◽  
Reflectometric Interference Spectroscopy ◽  
Parallel Screening

Label-free detection of C-reactive protein using reflectometric interference spectroscopy-based sensing system

2012 ◽  
Vol 728 ◽  
pp. 64-68 ◽  
Author(s):  
Hyung Woo Choi ◽  
Yasuhiko Sakata ◽  
Yoshikazu Kurihara ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Sensing System ◽  
Reflectometric Interference Spectroscopy ◽  
Label Free Detection

Label-free characterisation of oligonucleotide hybridisation using reflectometric interference spectroscopy

2005 ◽  
Vol 382 (8) ◽  
pp. 1889-1894 ◽  
Author(s):  
Florian Pröll ◽  
Bernd Möhrle ◽  
Michael Kumpf ◽  
Günter Gauglitz
Keyword(s):  
Label Free ◽  
Reflectometric Interference Spectroscopy

scholarly journals Antifungal susceptibility testing of Aspergillus niger on silicon microwells by intensity-based reflectometric interference spectroscopy

2019 ◽  
Author(s):  
Christopher Heuer ◽  
Heidi Leonard ◽  
Nadav Nitzan ◽  
Ariella Lavy-Alperovitch ◽  
Naama Massad-Ivanir ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Susceptibility Testing ◽  
Fungal Infections ◽  
Antifungal Susceptibility ◽  
Fungal Growth ◽  
Antifungal Susceptibility Testing ◽  
Label Free ◽  
Broth Microdilution ◽  
Silicon Phase ◽  
Reflectometric Interference Spectroscopy

AbstractThe increasing number of invasive fungal infections among immunocompromised patients and the emergence of antifungal resistant pathogens has resulted in the need for rapid and reliable antifungal susceptibility testing (AFST). Accelerating antifungal susceptibility testing allows for advanced treatment decisions and the reduction in future instances of antifungal resistance. In this work, we demonstrate the application of a silicon phase grating as sensor for the detection of growth of Aspergillus niger (A. niger) by intensity-based reflectometric interference spectroscopy and its use as an antifungal susceptibility test. The silicon gratings provide a solid-liquid interface to capture micron-sized Aspergillus conidia within microwell arrays. Fungal growth is optically tracked and detected by the reduction in the intensity of reflected light from the silicon grating. The growth of A. niger in the presence of various concentrations of the antifungal agents voriconazole and amphotericin B is investigated by intensity-based reflectometric interference spectroscopy and used for the determination of the minimal inhibitory concentrations (MIC), which are compared to standard broth microdilution testing. This assay allows for expedited detection of fungal growth and provides a label-free alternative to standard antifungal susceptibility testing methods, such as broth microdilution and agar diffusion methods.

Label-free characterization of cell adhesion using reflectometric interference spectroscopy (RIfS)

2005 ◽  
Vol 384 (2) ◽  
pp. 407-413 ◽  
Author(s):  
Bernd P. Möhrle ◽  
Karsten Köhler ◽  
Jan Jaehrling ◽  
Roland Brock ◽  
Günter Gauglitz
Keyword(s):  
Cell Adhesion ◽  
Label Free ◽  
Reflectometric Interference Spectroscopy

scholarly journals Single beam grating coupled interferometry: high resolution miniaturized label-free sensor for plate based parallel screening

2012 ◽  
Vol 20 (21) ◽  
pp. 23162 ◽  
Author(s):  
Daniel Patko ◽  
Kaspar Cottier ◽  
Andras Hamori ◽  
Robert Horvath
Keyword(s):  
High Resolution ◽  
Label Free ◽  
Single Beam ◽  
Parallel Screening

scholarly journals Subcutaneously Delivered Nano-Insulin Drug as Label Free Nanotheranostic

2021 ◽  
Author(s):  
Rıdvan Say ◽  
Almila Şenat ◽  
Özlem Biçen Ünlüer ◽  
Fahrettin Akyüz ◽  
Arzu Ersöz
Keyword(s):  
Liquid Chromatography ◽  
Insulin Receptor ◽  
Rat Liver ◽  
Insulin Receptors ◽  
Insulin Analog ◽  
Second Step ◽  
Label Free ◽  
Column Material ◽  
Reflectometric Interference Spectroscopy ◽  
The Relationship

Abstract In this study, it has aimed to understand the relationship between purified insulin and insulin receptor, also nanoinsulin and insulin receptors. The insulin receptor has separated from rat liver using a cryogel column material that is photosensitively insulin cross-linked in the Fast Protein Liquid Chromatography (FPLC) system based on the affinity between insulin and insulin receptor. In the second step, an isolated insulin receptor has used to synthesize insulin receptor cross-linked cryogels for purifying insulin from rats. Subcutaneously delivered nano-insulin drug has prepared from the purified insulin using AmiNoAcid (monomer) Decorated and Light Underpinning Conjugation Approach (ANADOLUCA) method. Lastly, Reflectometric Interference Spectroscopy (RIfS) study has performed to understand the interaction between purified insulin receptor and purified insulin, commercial insulin analog, and nano insulin. These studies have demonstrated that nano-insulin drugs can be effectively used as a theranostic platform to monitor affinity and blocking interactions of nanoprotein drug and its receptor.

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