An Automated On-Line Multidimensional HPLC System for Protein and Peptide Mapping with Integrated Sample Preparation

2002 ◽  
Vol 74 (4) ◽  
pp. 809-820 ◽  
Author(s):  
Knut Wagner ◽  
Tasso Miliotis ◽  
György Marko-Varga ◽  
Rainer Bischoff ◽  
Klaus K. Unger
Keyword(s):  
Sample Preparation ◽  
Peptide Mapping ◽  
Hplc System ◽  
On Line ◽  
Multidimensional Hplc

scholarly journals 14C AMS at Suerc: Improving QA Data with the 5MV Tandem and 250KV SSAMS

Radiocarbon ◽  
2010 ◽  
Vol 52 (2) ◽  
pp. 263-271 ◽  
Author(s):  
P Naysmith ◽  
G T Cook ◽  
S P H T Freeman ◽  
E M Scott ◽  
R Anderson ◽  
...  
Keyword(s):  
Quality Assurance ◽  
Sample Preparation ◽  
Mass Spectrometer ◽  
Random Access ◽  
Capacity Measurement ◽  
Tandem Accelerator ◽  
Standard Data ◽  
Accelerator Mass Spectrometer ◽  
On Line ◽  
Automated Data Acquisition

In 2003, a National Electrostatics Corporation (NEC) 5MV tandem accelerator mass spectrometer was installed at SUERC, providing the radiocarbon laboratory with 14C measurements to 4–5‰ repeatability. In 2007, a 250kV single-stage accelerator mass spectrometer (SSAMS) was added to provide additional 14C capability and is now the preferred system for 14C analysis. Changes to the technology and to our operations are evident in our copious quality assurance data: typically, we now use the 134-position MC-SNICS source, which is filled to capacity. Measurement of standards shows that spectrometer running without the complication of on-line δ13C evaluation is a good operational compromise. Currently, 3‰ 14C/13C measurements are routinely achieved for samples up to nearly 3 half-lives old by consistent sample preparation and an automated data acquisition algorithm with sample random access for measurement repeats. Background and known-age standard data are presented for the period 2003–2008 for the 5MV system and 2007–2008 for the SSAMS, to demonstrate the improvements in data quality.

N and P on-line meters: requirements, maintenance and stability

1996 ◽  
Vol 33 (1) ◽  
pp. 147-157 ◽  
Author(s):  
Henrik A. Thomsen ◽  
Kenneth Kisbye
Keyword(s):  
Sample Preparation ◽  
Response Time ◽  
Ammonium Nitrate ◽  
State Of The Art ◽  
List Price ◽  
User Friendliness ◽  
On Line ◽  
The Cost ◽  
Preparation Design

State-of-the-art on-line meters for determination of ammonium, nitrate and phosphate are presented. The on-line meters employ different measuring principles and are available in many different designs differing with respect to size, calibration and cleaning principle, user-friendliness, response time, reagent and sample consumption. A study of Danish experiences on several plants has been conducted. The list price of an on-line meter is between USD 8000 and USD 35,000. To this should be added the cost of sample preparation, design, installation and running-in. The yearly operating for one meter are in the range of USD 200-2500 and the manpower consumption is in the range of 1-5 hours/month. The accuracy obtained is only slightly smaller than the accuracy on collaborative laboratory analyses, which is sufficient for most control purposes.

An On-Line Isocratic HPLC System for the Analysis of PTH-Amino Acids on A Gas-Phase Sequencer

Proteins ◽  
1987 ◽  
pp. 383-394
Author(s):  
J. E. Shively ◽  
D. Hawke ◽  
R. M. Kutny ◽  
B. Krieger ◽  
J. L. Glajch
Keyword(s):  
Amino Acids ◽  
Gas Phase ◽  
Hplc System ◽  
On Line ◽  
Isocratic Hplc
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