Reversed-Phase Membrane Inlet Mass Spectrometry Applied to the Real-Time Monitoring of Low Molecular Weight Alcohols in Chloroform

2002 ◽  
Vol 74 (1) ◽  
pp. 300-304 ◽  
Author(s):  
Colin S. Creaser ◽  
David Gómez Lamarca ◽  
Jeffrey Brum ◽  
Christopher Werner ◽  
Anthony P. New ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Real Time ◽  
Reversed Phase ◽  
Low Molecular Weight ◽  
Real Time Monitoring ◽  
The Real ◽  
Membrane Inlet Mass Spectrometry

Observing the real time formation of phosphine-ligated gold clusters by electrospray ionization mass spectrometry

2017 ◽  
Vol 19 (26) ◽  
pp. 17187-17198 ◽  
Author(s):  
Marshall R. Ligare ◽  
Grant E. Johnson ◽  
Julia Laskin
Keyword(s):  
Mass Spectrometry ◽  
Electrospray Ionization ◽  
Real Time ◽  
Electrospray Ionization Mass Spectrometry ◽  
Gold Cluster ◽  
Gold Clusters ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Real Time Monitoring ◽  
The Real

Real-time monitoring of the gold cluster synthesis by electrospray ionization mass spectrometry reveals distinct formation pathways for Au8, Au9 and Au10 clusters.

scholarly journals Detection of low molecular weight adulterants in beverages by direct analysis in real time mass spectrometry

2016 ◽  
Vol 8 (14) ◽  
pp. 2971-2978 ◽  
Author(s):  
Edward Sisco ◽  
Jeffrey Dake
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Real Time ◽  
Direct Analysis ◽  
Low Molecular Weight

Direct Analysis in Real Time Mass Spectrometry (DART-MS) has been used to detect the presence of non-narcotic adulterants in beverages.

Direct analysis in real time mass spectrometry (DART-MS) of highly non-polar low molecular weight polyisobutylenes

2015 ◽  
Vol 50 (9) ◽  
pp. 1071-1078 ◽  
Author(s):  
Lajos Nagy ◽  
Tibor Nagy ◽  
György Deák ◽  
Ákos Kuki ◽  
Borbála Antal ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Molecular Weight ◽  
Real Time ◽  
Direct Analysis ◽  
Low Molecular Weight ◽  
Polar Low

Real time monitoring of a biogas digester with gas chromatography, near-infrared spectroscopy, and membrane-inlet mass spectrometry

2011 ◽  
Vol 102 (5) ◽  
pp. 4098-4103 ◽  
Author(s):  
Alastair James Ward ◽  
Emiliano Bruni ◽  
Morten K. Lykkegaard ◽  
Anders Feilberg ◽  
Anders P.S. Adamsen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Infrared Spectroscopy ◽  
Real Time ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Real Time Monitoring ◽  
Membrane Inlet Mass Spectrometry

Real Time Monitoring Mass Spectrometry

2012 ◽  
pp. 246-256
Author(s):  
Yasuaki Takada ◽  
Yuichiro Hashimoto ◽  
Hisashi Nagano ◽  
Masuyuki Sugiyama ◽  
Masuyoshi Yamada ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Real Time ◽  
Illicit Drugs ◽  
Environmental Pollutants ◽  
Chemical Warfare Agents ◽  
Ion Source ◽  
Real Time Monitoring ◽  
Monitoring Technology ◽  
The Real ◽  
Warfare Agents

Monitoring or detection of illicit chemicals has become one of the most important issues worldwide due to the spreading global use of explosives and illicit drugs. To improve security, the authors have started developing a real time monitoring technology based on mass spectrometry. In this technology, a sample gas is directly introduced into an ion source without any pre-treatments of the sample gas, and ions produced by the ion source are analyzed by a mass spectrometer. Various organic compounds can be detected by analyzing the mass number of the observed ions. The real time monitoring technology has been applied to monitor environmental pollutants such as Polychlorinated Biphenyls (PCBs) and to detect explosives, chemical warfare agents, and illicit drugs. High-throughput detection of an improvised explosive has also been successfully demonstrated by the real time monitoring technology.

scholarly journals Simultaneous Determination of Human Serum Albumin and Low-Molecular-Weight Thiols after Derivatization with Monobromobimane

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3321
Author(s):  
Katarzyna Kurpet ◽  
Rafał Głowacki ◽  
Grażyna Chwatko
Keyword(s):  
Molecular Weight ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Simultaneous Determination ◽  
Reversed Phase ◽  
Fluorescence Labeling ◽  
Detector Response ◽  
Low Molecular Weight

Biothiols are extremely powerful antioxidants that protect cells against the effects of oxidative stress. They are also considered relevant disease biomarkers, specifically risk factors for cardiovascular disease. In this paper, a new procedure for the simultaneous determination of human serum albumin and low-molecular-weight thiols in plasma is described. The method is based on the pre-column derivatization of analytes with a thiol-specific fluorescence labeling reagent, monobromobimane, followed by separation and quantification through reversed-phase high-performance liquid chromatography with fluorescence detection (excitation, 378 nm; emission, 492 nm). Prior to the derivatization step, the oxidized thiols are converted to their reduced forms by reductive cleavage with sodium borohydride. Linearity in the detector response for total thiols was observed in the following ranges: 1.76–30.0 mg mL−1 for human serum albumin, 0.29–5.0 nmol mL−1 for α-lipoic acid, 1.16–35 nmol mL−1 for glutathione, 9.83–450.0 nmol mL−1 for cysteine, 0.55–40.0 nmol mL−1 for homocysteine, 0.34–50.0 nmol mL−1 for N-acetyl-L-cysteine, and 1.45–45.0 nmol mL−1 for cysteinylglycine. Recovery values of 85.16–119.48% were recorded for all the analytes. The developed method is sensitive, repeatable, and linear within the expected ranges of total thiols. The devised procedure can be applied to plasma samples to monitor biochemical processes in various pathophysiological states.

scholarly journals Proteomic Determination of Low-Molecular-Weight Glutenin Subunit Composition in Aroona Near-Isogenic Lines and Standard Wheat Cultivars

2021 ◽  
Vol 22 (14) ◽  
pp. 7709
Author(s):  
Kyoungwon Cho ◽  
You-Ran Jang ◽  
Sun-Hyung Lim ◽  
Susan B. Altenbach ◽  
Yong Q. Gu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Allelic Variation ◽  
Reversed Phase ◽  
Glutenin Subunit ◽  
Low Molecular Weight ◽  
Near Isogenic Lines ◽  
Wheat Cultivars ◽  
Reliable Determination ◽  
Isogenic Lines

The low-molecular weight glutenin subunit (LMW-GS) composition of wheat (Triticum aestivum) flour has important effects on end-use quality. However, assessing the contributions of each LMW-GS to flour quality remains challenging because of the complex LMW-GS composition and allelic variation among wheat cultivars. Therefore, accurate and reliable determination of LMW-GS alleles in germplasm remains an important challenge for wheat breeding. In this study, we used an optimized reversed-phase HPLC method and proteomics approach comprising 2-D gels coupled with liquid chromatography–tandem mass spectrometry (MS/MS) to discriminate individual LMW-GSs corresponding to alleles encoded by the Glu-A3, Glu-B3, and Glu-D3 loci in the ‘Aroona’ cultivar and 12 ‘Aroona’ near-isogenic lines (ARILs), which contain unique LMW-GS alleles in the same genetic background. The LMW-GS separation patterns for ‘Aroona’ and ARILs on chromatograms and 2-D gels were consistent with those from a set of 10 standard wheat cultivars for Glu-3. Furthermore, 12 previously uncharacterized spots in ‘Aroona’ and ARILs were excised from 2-D gels, digested with chymotrypsin, and subjected to MS/MS. We identified their gene haplotypes and created a 2-D gel map of LMW-GS alleles in the germplasm for breeding and screening for desirable LMW-GS alleles for wheat quality improvement.

Sign in / Sign up

Export Citation Format

Share Document