Second derivative solid surface luminescence analysis of two-component liquid chromatography fractions

1984 ◽  
Vol 56 (7) ◽  
pp. 1183-1186 ◽  
Author(s):  
R. A. Dalterio ◽  
R. J. Hurtubise
Keyword(s):  
Liquid Chromatography ◽  
Solid Surface ◽  
Second Derivative ◽  
Luminescence Analysis ◽  
Two Component ◽  
Surface Luminescence

Applications and Interactions in Solid-Surface Luminescence Analysis

1989 ◽  
pp. 155-166 ◽  
Author(s):  
Robert J. Hurtubise ◽  
S. M. Ramasamy ◽  
Job M. Bello ◽  
Greg J. Burrell ◽  
Linda A. Citta
Keyword(s):  
Solid Surface ◽  
Luminescence Analysis ◽  
Surface Luminescence

scholarly journals Study of Thermal Effects in Two-Component Nonisothermal Liquid Chromatography Considering Thermally Insulated Columns

2018 ◽  
Vol 57 (44) ◽  
pp. 15084-15095 ◽  
Author(s):  
Jamil Ur Rehman ◽  
Adeel Muneer ◽  
Javeria N. Abbasi ◽  
Shamsul Qamar ◽  
Andreas Seidel-Morgenstern
Keyword(s):  
Liquid Chromatography ◽  
Thermal Effects ◽  
Two Component

scholarly journals Simultaneous evaluation of losartan and amlodipine besylate using second-derivative synchronous spectrofluorimetric technique and liquid chromatography with time-programmed fluorimetric detection

2019 ◽  
Vol 6 (4) ◽  
pp. 190310 ◽  
Author(s):  
Shereen Shalan ◽  
Jenny Jeehan Nasr
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase ◽  
Synchronous Fluorescence ◽  
Second Derivative ◽  
Fluorimetric Detection ◽  
Amlodipine Besylate ◽  
Simultaneous Evaluation ◽  
Synchronous Fluorescence Spectra ◽  
Synchronous Fluorimetry

This study is concerned with two sensitive, fast and reproducible approaches; namely, second-derivative synchronous fluorimetry (method I) and reversed phase high-performance liquid chromatography with fluorimetric detection (method II) for synchronized evaluation of losartan (LOS) and amlodipine besylate (AML). Method I is based on measuring second-derivative synchronous fluorescence spectra of LOS and AML at Δ λ = 80 nm in water. The experimental factors influencing the synchronous fluorescence of the considered compounds were sensibly adjusted. The chromatographic analysis was executed on a Nucleodur MN-C18 column of dimensions; 250 × 4.6 mm i.d. and 5 µm particle size). The fluorimetric detection was time-programmed at λ em = 440 nm for AML (0.0–7.5 min) and at λ em = 400 nm for LOS (7.5–10 min) after excitation at λ ex = 245 nm. The mobile phase is a blend of acetonitrile with 0.02 M phosphate buffer in a proportion of 45 : 55, pH 4.0, pumped using a flow rate of 1 ml min −1 . The calibration plots were established to be 0.1–4.0 µg ml −1 for both drugs in method I and 0.05–4.0 µg ml −1 for both drugs in method II. The study was extended to the evaluation of the two drugs in their co-formulated tablets.

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