Aluminum-coated cell for fluorescence signal enhancement

1983 ◽  
Vol 55 (14) ◽  
pp. 2464-2466 ◽  
Author(s):  
A. J. M. Vermorken ◽  
C. M. A. A. Goos ◽  
M. W. A. C. Hukkelhoven ◽  
M. Coelen
Keyword(s):  
Signal Enhancement ◽  
Fluorescence Signal ◽  
Coated Cell

scholarly journals Insight into factors affecting the presence, degree, and temporal stability of fluorescence intensification on ZnO nanorod ends

Nanoscale ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 1424-1436 ◽  
Author(s):  
Manpreet Singh ◽  
Ruibin Jiang ◽  
Heidi Coia ◽  
Daniel S. Choi ◽  
Anginelle Alabanza ◽  
...  
Keyword(s):  
Simulation Study ◽  
Temporal Stability ◽  
Signal Enhancement ◽  
Fluorescence Signal ◽  
Optical Parameters ◽  
Zno Nanorod ◽  
Factors Affecting ◽  
Insight Into

We have carried out a combined experimental and simulation study identifying the key physical and optical parameters affecting the biomolecular fluorescence signal enhancement measured on ZnO NRs.

Fluorescence Signal Enhancement of Polydiacetylene Vesicle Stacks

2011 ◽  
Vol 11 (7) ◽  
pp. 6203-6207 ◽  
Author(s):  
Hyun Choi ◽  
Insung S. Choi ◽  
Gil Sun Lee ◽  
Dong June Ahn
Keyword(s):  
Signal Enhancement ◽  
Fluorescence Signal

scholarly journals Fluorescence Signal Enhancement in Antibody Microarrays Using Lightguiding Nanowires

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 227
Author(s):  
Damiano Verardo ◽  
Leena Liljedahl ◽  
Corinna Richter ◽  
Björn Agnarsson ◽  
Ulrika Axelsson ◽  
...  
Keyword(s):  
Gallium Phosphide ◽  
Light Emission ◽  
Signal Enhancement ◽  
Detection Sensitivity ◽  
Fluorescence Signal ◽  
Single Chain ◽  
Protein Biomarkers ◽  
Antibody Microarrays ◽  
Flat Surfaces ◽  
Diagnostic Assays

Fluorescence-based detection assays play an essential role in the life sciences and medicine. To offer better detection sensitivity and lower limits of detection (LOD), there is a growing need for novel platforms with an improved readout capacity. In this context, substrates containing semiconductor nanowires may offer significant advantages, due to their proven light-emission enhancing, waveguiding properties, and increased surface area. To demonstrate and evaluate the potential of such nanowires in the context of diagnostic assays, we have in this work adopted a well-established single-chain fragment antibody-based assay, based on a protocol previously designed for biomarker detection using planar microarrays, to freestanding, SiO2-coated gallium phosphide nanowires. The assay was used for the detection of protein biomarkers in highly complex human serum at high dilution. The signal quality was quantified and compared with results obtained on conventional flat silicon and plastic substrates used in the established microarray applications. Our results show that using the nanowire-sensor platform in combination with conventional readout methods, improves the signal intensity, contrast, and signal-to-noise by more than one order of magnitude compared to flat surfaces. The results confirm the potential of lightguiding nanowires for signal enhancement and their capacity to improve the LOD of standard diagnostic assays.

Enhanced DNA detection using a multiple pulse pumping scheme with time-gating (MPPTG)

The Analyst ◽  
2018 ◽  
Vol 143 (12) ◽  
pp. 2819-2827 ◽  
Author(s):  
Joseph D. Kimball ◽  
Badri Maliwal ◽  
Sangram L. Raut ◽  
Hung Doan ◽  
Zhangatay Nurekeyev ◽  
...  
Keyword(s):  
Dna Detection ◽  
Signal Enhancement ◽  
Detection Methods ◽  
Fluorescence Signal ◽  
Multiple Pulse ◽  
Time Gating

Fluorescence signal enhancement induced by the binding of intercalators to DNA has been broadly utilized in various DNA detection methods.

Application of metal nanoparticles/porous silicon diffraction grating in rhodamine 6 G fluorescence signal enhancement

2017 ◽  
Vol 15 (11) ◽  
pp. 110501
Author(s):  
Jiajia Wang Jiajia Wang ◽  
Zhenhong Jia Zhenhong Jia ◽  
Changwu Lv Changwu Lv ◽  
Yanyu Li Yanyu Li
Keyword(s):  
Porous Silicon ◽  
Metal Nanoparticles ◽  
Diffraction Grating ◽  
Signal Enhancement ◽  
Fluorescence Signal

Ratio imaging of intracellular ion concentration

1992 ◽  
Vol 50 (2) ◽  
pp. 1160-1161
Author(s):  
Stephen R. Bolsover
Keyword(s):  
Individual Cell ◽  
Video Camera ◽  
Field Of View ◽  
Ion Concentration ◽  
Fluorescence Signal ◽  
Ion Imaging ◽  
Ratiometric Fluorescence ◽  
Ratio Imaging ◽  
The Mean ◽  
The Many

The field of intracellular ion concentration measurement expanded greatly in the 1980's due primarily to the development by Roger Tsien of ratiometric fluorescence dyes. These dyes have many applications, and in particular they make possible to image ion concentrations: to produce maps of the ion concentration within living cells. Ion imagers comprise a fluorescence microscope, an imaging light detector such as a video camera, and a computer system to process the fluorescence signal and display the map of ion concentration.Ion imaging can be used for two distinct purposes. In the first, the imager looks at a field of cells, measuring the mean ion concentration in each cell of the many in the field of view. One can then, for instance, challenge the cells with an agonist and examine the response of each individual cell. Ion imagers are not necessary for this sort of experiment: one can instead use a system that measures the mean ion concentration in a just one cell at any one time. However, they are very much more convenient.

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